Nanoparticle-Mediated Delivery of shRNA.VEGF-A Plasmids Regresses Corneal Neovascularization

Subconjunctival injection of antagomir-21 alleviates corneal neovascularization in a mouse model of alkali-burned cornea

“…In alkali-burned corneas, the nanoparticle-mediated delivery of shRNA to VEGF-A inhibited neovascularization [19]. However, the mechanism of VEGF production has not been completely elucidated.…”

Preparation of arginine–glycine–aspartic acid-modified biopolymeric nanoparticles containing epigalloccatechin-3-gallate for targeting vascular endothelial cells to inhibit corneal neovascularization

“…10,[32][33][34][35] Application of NPs for corneal NV is becoming more popular. 36,37 Curcumin, the yellow extract from Curcuma longa, presents anti-inflammatory and antiangiogenic activities. It has been loaded in methoxypolyethylene glycol-poly-ε-caprolactone NPs delivered to corneal NV rats as eyedrops, showing enhanced retention of curcumin in the cornea and significant improvement in prevention of corneal NV over free curcumin.…”

Dendrimers for gene delivery – a potential approach for ocular therapy?

“…Qazi et al [84] investigated poly(lactide-co-glycolide) (PLGA) nanoparticles prepared by the double emulsion-solvent evaporation method containing a small hairpin RNA expression cassette (pSEC.shRNA) against vascular endothelial growth factor (VEGF)-A in a mouse model of corneal neovascularization. They found that VEGF-A mRNA and protein expression were significantly reduced compared with control nanoparticles, rendering these particles a highly efficacious, sustainable and non-toxic treatment option for the prevention of neovascularization after corneal insult.…”

“…The dendrimer consistently yielded the highest gene transfer efficiency into primary human RPE cells, with the DNA concentration and DNA : dendrimer concentration playing a major role in the transfection efficiency. In the same year, Hudde et al [99] investigated activated PAMAM dendrimers for the delivery of plasmids [83] PLGA nanoparticles pSEC.shRNA (VEGF-A) Corneal neovascularization [84] Human serum albumin nanoparticles pDNA (SOD1) Age-related macular degeneration [85] CS-g-(PEI-b-mPEG) nanoparticles siRNA (IKKb) Bleb survival after trabeculectomy [86] PLA and PLGA nanoparticles pDNA (GFP, RNFP) Bovine primary RPE cells, human RPE cells (ARPE-19) [87] PLGA microspheres RNA aptamer (VEGF) Human umbilical vein endothelial cells (HUVEC) [88] Hyaluronic acid chitosan nanoparticles pDNA (EGFP or b-gal) Human corneal epithelial cells (HCEC) and normal human conjunctival (IOBA-NHC) cells [89] Calcium phosphate nanoparticles pDNA (EGFP) (with PEI) Immortalized human corneal endothelial cells (HCEC) [90] (B) Liposomes DOTAP/DOPE/PS/PEI pDNA (SEAP) Human RPE cells (ARPE-19) [91] DOTAP/DOPE/ DSPE-PEG or ceramide-PEG pDNA (Luciferase) Human RPE cells (407) [92] DOTMA/DOPE or DOTMA/cholesterol pDNA (Luciferase) Rabbit eye [93] Phospholipid/PEG-DSPE/cholesterol ODN Rabbit eye [94] (C) Dendrimers PLL dendrimers ODN (VEGF) Choroidal neovascularization [95][96][97] PAMAM dendrimers pDNA (GFP) Human primary RPE cells [98] PAMAM dendrimers pDNA (TNFR-Ig) Human or rabbit corneas [99] PLL and PEG-PLL dendrimers pDNA (Luciferase) Human RPE cells (D407) [100] (D) Others PEI PS-AsODN (anti-TGFb2) Rat retinal Müller glial cells [101] Cell penetrating peptide pDNA (GPFHis) Human embryonic retinoblast (HER) cells [102] Dihydrazide derivatized hyaluronic acid Nucleic acid (HAS) Dry eye syndrome [103] Cationic nanoemulsion AsODN (VEGF-R2) Corneal neovascularization [104] Cationic core-shell liponanoparticles pDNA (EGFP) Human conjunctival epithelial cells and rabbit eye [105] Electrotransfer to ciliary muscle pDNA (hTNFR-Is) Inflammatory, degenerative or angiogenic diseases [106] Ultrasound with DOTAP/DOPE/ DSPE-PEG liposomes pDNA (Luciferse) Bovine neural retina [107]…”