Nanoparticles for nucleic acid delivery: Applications in cancer immunotherapy

Mukalel, Alvin J.; Riley, Rachel; Zhang, Rui; Mitchell, Michael J.

doi:10.1016/j.canlet.2019.04.040

Cited by 98 publications

(69 citation statements)

References 133 publications

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“…In the immune field, biomaterials are used to deliver a range of vectors that either encode immune signals or bind complementary nucleic acid sequences involved in immune function; alternatively, the cargo are nucleic acid‐based ligands—such as TLR agonists—that trigger innate immune pathways, but do not directly encode genetic information (see Section ). Thus, precision delivery of DNA, messenger RNA (mRNA), microRNA (miR), small interfering RNA (siRNA), and other genetic molecules offer multiple levels to probe how the trafficking and processing of immune signals impacts immunity and regulation . The delivery of these different classes of nucleic acid will be discussed in this section.…”

Section: Biomaterials Enable Analysis Of Immune Signaling Through Conmentioning

confidence: 99%

“…Thus, precision delivery of DNA, messenger RNA (mRNA), microRNA (miR), small interfering RNA (siRNA), and other genetic molecules offer multiple levels to probe how the trafficking and processing of immune signals impacts immunity and regulation. [68][69][70][71][72] The delivery of these different classes of nucleic acid will be discussed in this section. Along these lines, innate immune cells have historically proven difficult to successfully transfect because of the efficiency with which these cells degrade nucleic acid and the limited proliferative capacity of these cells.…”

Section: Using Biomaterials To Guide the Delivery Of Immune Signals Tmentioning

confidence: 99%

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Biomaterials as Tools to Decode Immunity

Eppler¹,

Jewell

2019

Advanced Materials

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The immune system has remarkable capabilities to combat disease with exquisite selectivity. This feature has enabled vaccines that provide protection for decades and, more recently, advances in immunotherapies that can cure some cancers. Greater control over how immune signals are presented, delivered, and processed will help drive even more powerful options that are also safe. Such advances will be underpinned by new tools that probe how immune signals are integrated by immune cells and tissues. Biomaterials are valuable resources to support this goal, offering robust, tunable properties. The growing role of biomaterials as tools to dissect immune function in fundamental and translational contexts is highlighted. These technologies can serve as tools to understand the immune system across molecular, cellular, and tissue length scales. A common theme is exploiting biomaterial features to rationally direct how specific immune cells or organs encounter a signal. This precision strategy, enabled by distinct material properties, allows isolation of immunological parameters or processes in a way that is challenging with conventional approaches. The utility of these capabilities is demonstrated through examples in vaccines for infectious disease and cancer immunotherapy, as well as settings of immune regulation that include autoimmunity and transplantation.

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Section: Biomaterials Enable Analysis Of Immune Signaling Through Conmentioning

confidence: 99%

Section: Using Biomaterials To Guide the Delivery Of Immune Signals Tmentioning

confidence: 99%

Biomaterials as Tools to Decode Immunity

Eppler¹,

Jewell

2019

Advanced Materials

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“…In the present study, different preparation methods were explored to produce stable nanoemulsions to be used as adjuvants for anti-serum production for the treatment of the Tityus serrulatus scorpion sting. Distinct compositions and formulation approaches, including the cationic nanoemulsions covered with the hyperbranched polyethyleneimine were tested, an FDA approved biocompatible material capable to enhance nucleic acids, genes and drugs delivery [ 42 , 43 , 44 ]. The physicochemical properties of all formulations were carefully followed aiming to establish a nanotechnology platform for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Cationic-Covered Nanoemulsion as A Novel Biocompatible Immunoadjuvant for Antiserum Production Against Tityus serrulatus Scorpion Venom

et al. 2020

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This study assesses the efficacy of different nanoemulsion formulations as new and innovative adjuvants for improving the in vivo immunization against the Tityus serrulatus scorpion venom. Nanoemulsions were designed testing key-variables such as surfactants, co-solvents, and the influence of the temperature, which would be able to induce the phase transition from a liquid crystal to a stable nanoemulsion, assessed for four months. Additionally, cationic-covered nanoemulsion with hyper-branched poly(ethyleneimine) was prepared and its performance was compared to the non-cationic ones. The physicochemical properties of the selected nanoemulsions and the interactions among their involved formulation compounds were carefully monitored. The cytotoxicity studies in murine macrophages (RAW 264.7) and red blood cells were used to compare different formulations. Moreover, the performance of the nanoemulsion systems as biocompatible adjuvants was evaluated using mice immunization protocol. The FTIR shifts and the zeta potential changes (from −18.3 ± 1.0 to + 8.4 ± 1.4) corroborated with the expected supramolecular anchoring of venom proteins on the surface of the nanoemulsion droplets. Cell culture assays demonstrated the non-toxicity of the formulations at concentrations less than 1.0 mg/mL, which were able to inhibit the hemolytic effect of the scorpion venom. The cationic-covered nanoemulsion has shown superior adjuvant activity, revealing the highest IgG titer in the immunized animals compared to both the non-cationic counterpart and the traditional aluminum adjuvant. In this approach, we demonstrate the incredible potential application of nanoemulsions as adjuvants, using a nanotechnology platform for antigen delivery system on immune cells. Additionally, the functionalization with hyper-branched poly(ethyleneimine) enhances this recognition and improves its action in immunization.

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“…co-incubation of liposome/DNA complexes with albumin, chitosan, or protamine not only reduced degradation but also enhanced DNA transfer efficiency [14,[16][17][18][19]. Especially for in vivo applications, various types of nanoparticles have also been developed to stabilize and protect nucleic acids and to facilitate cell type-specific targeting [20].Common drawbacks of liposome-or polymer-based transfection methods are sometimes low transfer rates [21,22] combined with significant cytotoxicity [23,24]. Both limitations are caused by the underlying uptake pathway via endocytosis.…”

mentioning

confidence: 99%

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confidence: 99%

Complex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes

Hoffmann

Hersch

Gerlach

et al. 2020

IJMS

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Highly efficient, biocompatible, and fast nucleic acid delivery methods are essential for biomedical applications and research. At present, two main strategies are used to this end. In non-viral transfection liposome- or polymer-based formulations are used to transfer cargo into cells via endocytosis, whereas viral carriers enable direct nucleic acid delivery into the cell cytoplasm. Here, we introduce a new generation of liposomes for nucleic acid delivery, which immediately fuse with the cellular plasma membrane upon contact to transfer the functional nucleic acid directly into the cell cytoplasm. For maximum fusion efficiency combined with high cargo transfer, nucleic acids had to be complexed and partially neutralized before incorporation into fusogenic liposomes. Among the various neutralization agents tested, small, linear, and positively charged polymers yielded the best complex properties. Systematic variation of liposomal composition and nucleic acid complexation identified surface charge as well as particle size as essential parameters for cargo-liposome interaction and subsequent fusion induction. Optimized protocols were tested for the efficient transfer of different kinds of nucleic acids like plasmid DNA, messenger RNA, and short-interfering RNA into various mammalian cells in culture and into primary tissues.

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Nanoparticles for nucleic acid delivery: Applications in cancer immunotherapy

Cited by 98 publications

References 133 publications

Biomaterials as Tools to Decode Immunity

Biomaterials as Tools to Decode Immunity

Self-Assembled Cationic-Covered Nanoemulsion as A Novel Biocompatible Immunoadjuvant for Antiserum Production Against Tityus serrulatus Scorpion Venom

Complex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes

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