Nanoparticles Production and Inclusion in &amp;lt;i&amp;gt;S. aureus&amp;lt;/i&amp;gt; Incubated with Polyurethane: An Electron Microscopy Analysis

Didenko, L. V.; Avtandilov, G. A.; Shevlyagina, N. V.; Shustrova, N. M.; Smirnova, Tatiana A.; Лебеденко, И. Ю.; Curia, Roberta; Savoia, Claudio; Tatti, Francesco; Milani, M.

doi:10.4236/ojmi.2013.32010

Cited by 4 publications

(4 citation statements)

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“…TEM is a complementary technique, useful to investigate the samples inner structure, composition and a suitable set of correlated instruments for this multi‐facet investigation [37, 38]. We used TEM to obtain distinct images in order to investigate the morphological changes in bacteria after treatment with F‐MWNTs.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial activity of functionalised carbon nanotubes against pathogenic microorganisms

Abo-Neima

Motaweh

Elsehly

2020

IET nanobiotechnol.

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Carbon nanotubes represent one of the best examples of novel nanostructures, exhibit a range of extraordinary physical properties, strong antimicrobial activity and can pierce bacterial cell walls. This investigation handles the antimicrobial activity of functionalised multiwall carbon nanotubes (F-MWNTs) as an alternative antimicrobial material compared to the commercial antibiotics. Antibacterial activities of F-MWNTs are investigated through two different kinds of bacteria, E. coli and S. aureus. The results demonstrate that the best concentration of F-MWNTs for the maximum inhibition and antibacterial functionality is 80 and 60 μg/ml for E. coli and S. aureus, respectively. The transmission electron microscope reveals the morphological changes damage mechanism for the cellular reliability on these microorganisms. F-MWNTs are capable of biologically isolating the cell from their microenvironment, contributing to the development of toxic substances and placing the cell under oxidative stress leading to cellular death. The efficiency of F-MWNTs is compared with the common antibiotics and shows an enhancement in the inhibitory effect with percentages reaches 85%. To account for the bactericidal performance of F-MWNTs towards these pathogens, the dielectric conductivity and the bacterial growth measurements are conducted. The present study endeavour that F-MWNTs could be exploited in biomedical devices and altering systems for hospital and industrial cleaning applications.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial activity of functionalised carbon nanotubes against pathogenic microorganisms

Abo-Neima

Motaweh

Elsehly

2020

IET nanobiotechnol.

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“…Biofilm maturation is accompanied by the appearance of an exopolysaccharide (EPS) matrix. Bacteria embedded in biofilm operate a corrosive action on the plastic surface, becoming the PU a feeding source for the microorganisms [8,9], and as a result the PU surface results seriously damaged [7,10,11].…”

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confidence: 99%

“…FIB/SEM images visible in Didenko et al [7] document that as a consequence of the biodestructive action of S. aureus, micro-and nano-particles (which size ranges from 2-3 nm to 10 nm) detach themselves from the bulk PU and are embedded in the exopolysaccharide (EPS) matrix. Through TEM and STEM images in Didenko et al [10] and Curia et al [11] it is possible to detect PU NPs within S. aureus.…”

mentioning

confidence: 99%

“…During the endocytic process, xenobiotic material is internalized by a cell through the invagination of the plasma membrane and the scission of MVs that envelop the external material [17]. Figure 1A shows exactly these steps: we can see free NPs (not surrounded by a MV) out of the cell, and MVs enveloping PU NPs within the bacterium; furthermore details of the membrane lysis and a ruffle are visible as well [7,10,11].…”

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confidence: 99%

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Study of the Formation, Secretion and Trafficking of Membrane Vesicles Loaded with Polyurethane Nanoparticles in Staphylococcus aureus via Electron Microscopy

Curia¹

2015

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In the last decade great interest has been demonstrated towards nanoparticles (NPs) especially for their unique characteristics which make NPs a powerful tool to work with in several fields, from engineering to medicine, from biology to materials sciences, from environmental sciences to (nano) toxicology [1]. In literature studies take into account different categories of NPs (metallic and non-metallic NPs, inert and reactive NPs); most of the papers talk about engineered NPs absorbed by inhalation, ingestion, via dermal penetration or intravenous perfusion [1]. The reason why NPs are widely used in so many fields of application is that they have a high surface/volume ratio, depending on the species they may be highly reactive, have unique physical and chemical properties, are able to easily pass through biological membranes, and finally they can be used for drug delivery [2,3]. All these characteristics on one hand make NPs useful, on the other hand NPs can turn out to be risky and harmful to handle, raising important issues about their toxicity, bioaccumulation and NPs-induced pathologies [4-6].

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Analysis of the Bacterial Vesicles' Enhanced Toxicological Threat Via Electron Microscopy

Curia

Milani

Didenko

et al. 2017

Advancing Medicine Through Nanotechnology and Nanomechanics Applications

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This study shows the importance of electron microscopy in the analysis of the interaction of microorganisms (Staphylococcus aureus) with polymeric (polyurethane) dental prostheses. Starting from the biofilm formation and the biodestruction of the plastic material resulting in the production of polyurethane nanoparticles, the focus is on the bacterial secretion of membrane vesicles (in the range of 20-50 nm) loaded with plastic nanoparticles (from 2-3 to 10 nm) and on the toxicological threat that these delivery devices represent when interacting with host cells. The nanoparticles deliverance led by the bacterial infections dynamics opens new ways to the possibility of delivering drugs to selected cells.

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Nanoparticles Production and Inclusion in <i>S. aureus</i> Incubated with Polyurethane: An Electron Microscopy Analysis

Cited by 4 publications

References 14 publications

Antimicrobial activity of functionalised carbon nanotubes against pathogenic microorganisms

Antimicrobial activity of functionalised carbon nanotubes against pathogenic microorganisms

Study of the Formation, Secretion and Trafficking of Membrane Vesicles Loaded with Polyurethane Nanoparticles in Staphylococcus aureus via Electron Microscopy

Analysis of the Bacterial Vesicles' Enhanced Toxicological Threat Via Electron Microscopy

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