Nanoparticulate Drug-Delivery Systems for Fighting Microbial Biofilms: From Bench to Bedside

Pîrcălăbioru, Grațiela Grădișteanu; Chifiriuc, Mariana‐Carmen

doi:10.2217/fmb-2019-0251

Cited by 41 publications

(42 citation statements)

References 132 publications

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“…Nanotechnology finds nowadays applications in almost all fields of human activity, including pharmaceutical and biomedical ones. Various nanomaterials are used to fight planktonic bacteria and biofilms due to their intrinsic antibacterial and anti-pathogenic potential, as well as to their capacity to be used as delivery systems for natural compounds or synthetic drugs [29].…”

Section: Discussionmentioning

confidence: 99%

Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains

et al. 2021

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Efficient antibiotics to cure Pseudomonas aeruginosa persistent infections are currently insufficient and alternative options are needed. A promising lead is to design therapeutics able to modulate key phenotypes in microbial virulence and thus control the progression of the infectious process without selecting resistant mutants. In this study, we developed a nanostructured system based on Fe3O4 nanoparticles (NPs) and eugenol, a natural plant-compound which has been previously shown to interfere with microbial virulence when utilized in subinhibitory concentrations. The obtained functional NPs are crystalline, with a spherical shape and 10–15 nm in size. The subinhibitory concentrations (MIC 1/2) of the eugenol embedded magnetite NPs (Fe3O4@EUG) modulate key virulence phenotypes, such as attachment, biofilm formation, persister selection by ciprofloxacin, and the production of soluble enzymes. To our knowledge, this is the first report on the ability of functional magnetite NPs to modulate P. aeruginosa virulence and phenotypic resistance; our data highlights the potential of these bioactive nanostructures to be used as anti-pathogenic agents.

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Section: Discussionmentioning

confidence: 99%

Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains

et al. 2021

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“…A pivotal but still underestimated contribution to the dimension of AR problem is brought by microbial biofilms developed on cellular/tissue substrates or medical devices ( Lazãr and Chifiriuc, 2010 ; Pircalabioru and Chifiriuc, 2020 ). These microbial communities or biofilms represent a form of existence with a particular architecture and behavior, different from that of single, free-floating, or planktonic cells living in citadels challenging to conquer ( Lazãr and Chifiriuc, 2010 ; Lazar, 2011 ), which is also more advantageous for bacteria, mainly due to the intercellular communication and sense of their density inside biofilms and exhibiting high phenotypic resistance (or tolerance) to high doses of antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Quorum Sensing and Biofilms in Less Investigated Gram-Negative ESKAPE Pathogens

et al. 2021

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Pathogenic bacteria have the ability to sense their versatile environment and adapt by behavioral changes both to the external reservoirs and the infected host, which, in response to microbial colonization, mobilizes equally sophisticated anti-infectious strategies. One of the most important adaptive processes is the ability of pathogenic bacteria to turn from the free, floating, or planktonic state to the adherent one and to develop biofilms on alive and inert substrata; this social lifestyle, based on very complex communication networks, namely, the quorum sensing (QS) and response system, confers them an increased phenotypic or behavioral resistance to different stress factors, including host defense mechanisms and antibiotics. As a consequence, biofilm infections can be difficult to diagnose and treat, requiring complex multidrug therapeutic regimens, which often fail to resolve the infection. One of the most promising avenues for discovering novel and efficient antibiofilm strategies is targeting individual cells and their QS mechanisms. A huge amount of data related to the inhibition of QS and biofilm formation in pathogenic bacteria have been obtained using the well-established gram-positive Staphylococcus aureus and gram-negative Pseudomonas aeruginosa models. The purpose of this paper was to revise the progress on the development of antibiofilm and anti-QS strategies in the less investigated gram-negative ESKAPE pathogens Klebsiella pneumoniae, Acinetobacter baumannii, and Enterobacter sp. and identify promising leads for the therapeutic management of these clinically significant and highly resistant opportunistic pathogens.

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“…Biofilms are the group of organized colonies of microbial species comprising fungi, bacteria, and yeasts that develop a syntrophic association with their adherence to the biotic and abiotic surfaces by self-encapsulating extracellular polymeric substances (EPSs) ( Costerton et al, 1995 ). The microcolonies existing within the EPS interact via the mechanism of quorum sensing (QS) that specifically helps in the development of the biofilm and the expression of virulence ( Pircalabioru and Chifiriuc, 2020 ). The phenotypic and genotypic expressions of the sessile cells differ from the planktonic forms and are majorly associated with the development of resistances against antibiotics.…”

Section: Introductionmentioning

confidence: 99%

“…The field of nanotechnology involves scientific and engineering technologies that aim to synthesize various materials of nano-dimensions that have wide applications in the fields of bioprospecting, pharmaceuticals, human activities, and biomedical applications. The development of nanomaterials is a new and promising strategy for acting as therapeutic agents against various types of biofilm-associated pathogenic infections that are associated with implants and medical devices ( Pircalabioru and Chifiriuc, 2020 ). Various types of nanomaterials have been associated to combat against various biofilms due to their prevailing properties which are microbiostatic, microbiocidal, and antipathogenic in nature and because they can be used for the purpose of delivering synthetic drugs and natural compounds ( Grumezescu and Chifiriuc, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Microbial Fabrication of Nanomaterial and Its Role in Disintegration of Exopolymeric Matrices of Biofilm

et al. 2021

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Bacterial biofilms are responsible for the development of various chronic wound-related and implant-mediated infections and confer protection to the pathogenic bacteria against antimicrobial drugs and host immune responses. Hence, biofilm-mediated chronic infections have created a tremendous burden upon healthcare systems worldwide. The development of biofilms upon the surface of medical implants has resulted in the failure of various implant-based surgeries and therapies. Although different conventional chemical and physical agents are used as antimicrobials, they fail to kill the sessile forms of bacterial pathogens due to the resistance exerted by the exopolysaccharide (EPS) matrices of the biofilm. One of the major techniques used in addressing such a problem is to directly check the biofilm formation by the use of novel antibiofilm materials, local drug delivery, and device-associated surface modifications, but the success of these techniques is still limited. The immense expansion in the field of nanoscience and nanotechnology has resulted in the development of novel nanomaterials as biocidal agents that can be either easily integrated within biomaterials to prevent the colonization of microbial cells or directly approach the pathogen overcoming the biofilm matrix. The antibiofilm efficacies of these nanomaterials are accomplished by the generation of oxidative stresses and through alterations of the genetic expressions. Microorganism-assisted synthesis of nanomaterials paved the path to success in such therapeutic approaches and is found to be more acceptable for its “greener” approach. Metallic nanoparticles functionalized with microbial enzymes, silver–platinum nanohybrids (AgPtNHs), bacterial nanowires, superparamagnetic iron oxide (Fe3O4), and nanoparticles synthesized by both magnetotactic and non-magnetotactic bacteria showed are some of the examples of such agents used to attack the EPS.

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Nanoparticulate Drug-Delivery Systems for Fighting Microbial Biofilms: From Bench to Bedside

Cited by 41 publications

References 132 publications

Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains

Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains

Modulation of Quorum Sensing and Biofilms in Less Investigated Gram-Negative ESKAPE Pathogens

Microbial Fabrication of Nanomaterial and Its Role in Disintegration of Exopolymeric Matrices of Biofilm

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