Nanoparticulates for Antibiofilm Treatment and Effect of Aging on Its Antibacterial Activity

Shrestha, Annie; Shi, Zhijun; Neoh, K. G.; Kishen, Anil

doi:10.1016/j.joen.2010.02.008

Cited by 233 publications

(181 citation statements)

References 34 publications

Supporting

Mentioning

156

Contrasting

Unclassified

Order By: Relevance

“…It shows a broad range of antimicrobial activity and has biocompatible and biodegradable properties (24,37). Chitosan has been subjected to numerous chemical modifications and grafting procedures due to its large number of free hydroxyl and amino groups (4,24).…”

mentioning

confidence: 99%

Characterization of a Conjugate between Rose Bengal and Chitosan for Targeted Antibiofilm and Tissue Stabilization Effects as a Potential Treatment of Infected Dentin

Shrestha

Hamblin

Kishen

2012

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

Bacterial biofilms and dentin structural changes are some of the major challenges in the management of infected dentin tissue. This study characterized a photosensitizer-conjugated chitosan with enhanced photodynamic efficacy against dental biofilms, as well as the ability to reinforce the postinfected dentin matrix in order to improve its mechanical and chemical stability. Rose Bengal-conjugated chitosan (CSRB) was synthesized using a chemical cross-linking method and characterized for photophysical, photobiological, and cytotoxicity properties. Its potential as an antibacterial and matrix-reinforcing agent on dentin collagen was also evaluated. Enterococcus faecalis as planktonic and in vitro biofilms was treated with CSRB and photodynamically activated with 5 to 60 J/cm 2 green light. Dentin collagen was used for the CSRB cross-linking experiments and evaluated for chemical changes, resistance to enzymatic degradation, and mechanical properties. CSRB was a photosensitizer with efficient singlet oxygen yield. In vitro photoactivation gave higher fibroblast cell survival than did RB alone. CSRB showed significant antibiofilm photoinactivation (P < 0.01). The CSRB-cross-linked dentin collagen showed higher resistance to collagenase degradation and superior mechanical properties (P < 0.05). In summary, the photoactivated CSRB particles synthesized in this study may be a synergistic multifunctional treatment approach with lower cytotoxicity and effective antibiofilm activity as well as the ability to reinforce the dentin collagen to enhance resistance to degradation and improve mechanical properties. This may be a targeted treatment strategy to deal with infected dentin hard tissues in a clinical scenario, where both disinfection and structural integrity need to be addressed concomitantly.

show abstract

mentioning

confidence: 99%

Characterization of a Conjugate between Rose Bengal and Chitosan for Targeted Antibiofilm and Tissue Stabilization Effects as a Potential Treatment of Infected Dentin

Shrestha

Hamblin

Kishen

2012

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

show abstract

“…Shrestha et al 24 evaluated nanoparticles of ZnO and chitosan against E. faecalis biofilm, and observed that ZnO was capable of reducing and destructuring the biofilm, with its efficiency being dependent on concentration and interaction time. ZnO is used in the composition of CH pastes due its radiopacifying and antibacterial properties.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of intracanal medications based on calcium hydroxide and zinc oxide micro- or nanoparticles: an ex vivo study

Guerreiro-Tanomaru

Aguiar

Chávez-Andrade

et al. 2017

Rev. odontol. UNESP

View full text Add to dashboard Cite

Introduction Nanoparticles and associations to calcium hydroxide can be used to increase antimicrobial activity. Objective To evaluate antibacterial activity against Enterococcus faecalis and pH of intracanal medications (IM) based on calcium hydroxide (CH) and zinc oxide (ZnO) micro- or nanoparticles, and their association with 0.4% chlorhexidine (CHX). Material and method Root canals from single-rooted human teeth were inoculated and incubated for 21 days. After sample (S1), the root canals were filled with the medications for 7 days and samples were collected immediately after medication (S2) and 7 days later (S3). Counting of CFU mL-1 was performed. Polyethylene tubes filled with the medications were used for the pH evaluation after 3, 7, 14 and 28 days. Data were submitted to ANOVA and Tukey tests (p<0.05). Result All IM promoted elimination of E. faecalis immediately after medication (S2). All the pastes promoted a similar pH increase. Conclusion CH/ZnO micro- or nanoparticles associated with CHX promoted greater bacterial reduction in the root canals and similar pH.

show abstract

“…Nanoparticles associated with chitosan (CS-np) and zinc oxide (ZnO-np) have demonstrated high efficacy in disorganizing the biofilm structure and retaining the antibacterial property for a long period of time [113]. In addition, combinations of medicaments and/or techniques, including new classes of photosensitizers associated with nanoparticles and PDT, have been proposed.…”

Section: Biofilm Associated With Endodontic Infectionsmentioning

confidence: 99%

Microbiology of Oral Biofilm-Dependent Diseases: Have We Made Significant Progress to Understand and Treat These Diseases?

Colombo

Souto

Silva-Boghossian

et al. 2014

Curr Oral Health Rep

View full text Add to dashboard Cite

The oral microbiome plays a crucial role in the establishment and maintenance of oral health. Major disturbances in the interactions between this microbiome, the microenvironment, and the host may lead to the development of oral diseases. A large proportion of infectious diseases that affect humans are caused by organisms living in biofilms. This is also the case for oral infections such as dental caries, periodontal diseases, and endodontic and fungal infections. In this review, new concepts regarding the role of the oral biofilm in the etiopathogenesis of these diseases are presented, and a more ecological view of pathogenic communities as causative agents of oral diseases is discussed. We believe that a better understanding of oral biofilm physiology and ecology will provide insight for the development of new cost-effective diagnostic tools, as well as preventive and therapeutic strategies for the management of these infections.

show abstract

Nanoparticulates for Antibiofilm Treatment and Effect of Aging on Its Antibacterial Activity

Cited by 233 publications

References 34 publications

Characterization of a Conjugate between Rose Bengal and Chitosan for Targeted Antibiofilm and Tissue Stabilization Effects as a Potential Treatment of Infected Dentin

Characterization of a Conjugate between Rose Bengal and Chitosan for Targeted Antibiofilm and Tissue Stabilization Effects as a Potential Treatment of Infected Dentin

Antibacterial activity of intracanal medications based on calcium hydroxide and zinc oxide micro- or nanoparticles: an ex vivo study

Microbiology of Oral Biofilm-Dependent Diseases: Have We Made Significant Progress to Understand and Treat These Diseases?

Contact Info

Product

Resources

About