Nanopatterned Surfaces for Biomedical Applications

McMurray, Rebecca J.; Dalby, Matthew J.; Gadegaard, Nikolaj

doi:10.5772/13453

Cited by 12 publications

(7 citation statements)

References 64 publications

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“…Then in the development step, exposed patterns are revealed. In the EBL process, conducting substrates or metallic films coated non-conducting substrates are used [ 66 ].…”

Section: Fabrication Of Micro-nanostructures and Substrates For 2d Stmentioning

confidence: 99%

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Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

Ermis

Antmen

Hasırcı

2018

Bioactive Materials

236

169

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Cell-substrate interactions play a crucial role in the design of better biomaterials and integration of implants with the tissues. Adhesion is the binding process of the cells to the substrate through interactions between the surface molecules of the cell membrane and the substrate. There are several factors that affect cell adhesion including substrate surface chemistry, topography, and stiffness. These factors physically and chemically guide and influence the adhesion strength, spreading, shape and fate of the cell. Recently, technological advances enabled us to precisely engineer the geometry and chemistry of substrate surfaces enabling the control of the interaction cells with the substrate. Some of the most commonly used surface engineering methods for eliciting the desired cellular responses on biomaterials are photolithography, electron beam lithography, microcontact printing, and microfluidics. These methods allow production of nano- and micron level substrate features that can control cell adhesion, migration, differentiation, shape of the cells and the nuclei as well as measurement of the forces involved in such activities. This review aims to summarize the current techniques and associate these techniques with cellular responses in order to emphasize the effect of chemistry, dimensions, density and design of surface patterns on cell-substrate interactions. We conclude with future projections in the field of cell-substrate interactions in the hope of providing an outlook for the future studies.

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“…Then in the development step, exposed patterns are revealed. In the EBL process, conducting substrates or metallic films coated non-conducting substrates are used [ 66 ].…”

Section: Fabrication Of Micro-nanostructures and Substrates For 2d Stmentioning

confidence: 99%

“…When cold, the master is remove from the polymer replica. It is a straightforward and short procedure [ 66 ].…”

Section: Fabrication Of Micro-nanostructures and Substrates For 2d Stmentioning

confidence: 99%

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

Ermis

Antmen

Hasırcı

2018

Bioactive Materials

236

169

View full text Add to dashboard Cite

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“…Since the topographies are partially embedded in the substrate surface, it is neither 2D or 3D and hence 2.5D. Microfabricated 2.5D platforms, made with techniques such as hot embossing, thermoforming, photolithography, soft lithography, or electron beam lithography, provide cells with a structurally more representative biomimetic environment compared to 2D substrates, while still retaining a high degree of control over parameters such as material, coating, roughness, stiffness, and dimensions of features. − 2.5D platforms are rapidly gaining popularity and show great promise in unraveling the influence of substrate geometry on cell behavior. ,− …”

Section: Introductionmentioning

confidence: 99%

Protein Micropatterning in 2.5D: An Approach to Investigate Cellular Responses in Multi-Cue Environments

Putten

Buskermolen

Werner

et al. 2021

ACS Appl. Mater. Interfaces

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The extracellular microenvironment is an important regulator of cell functions. Numerous structural cues present in the cellular microenvironment, such as ligand distribution and substrate topography, have been shown to influence cell behavior. However, the roles of these cues are often studied individually using simplified, single-cue platforms that lack the complexity of the three-dimensional, multi-cue environment cells encounter in vivo. Developing ways to bridge this gap, while still allowing mechanistic investigation into the cellular response, represents a critical step to advance the field. Here, we present a new approach to address this need by combining optics-based protein patterning and lithography-based substrate microfabrication, which enables high-throughput investigation of complex cellular environments. Using a contactless and maskless UV-projection system, we created patterns of extracellular proteins (resembling contact-guidance cues) on a two-and-a-half-dimensional (2.5D) cell culture chip containing a library of well-defined microstructures (resembling topographical cues). As a first step, we optimized experimental parameters of the patterning protocol for the patterning of protein matrixes on planar and non-planar (2.5D cell culture chip) substrates and tested the technique with adherent cells (human bone marrow stromal cells). Next, we fine-tuned protein incubation conditions for two different vascular-derived human cell types (myofibroblasts and umbilical vein endothelial cells) and quantified the orientation response of these cells on the 2.5D, physiologically relevant multi-cue environments. On concave, patterned structures (curvatures between κ = 1/2500 and κ = 1/125 μm–1), both cell types predominantly oriented in the direction of the contact-guidance pattern. In contrast, for human myofibroblasts on micropatterned convex substrates with higher curvatures (κ ≥ 1/1000 μm–1), the majority of cells aligned along the longitudinal direction of the 2.5D features, indicating that these cells followed the structural cues from the substrate curvature instead. These findings exemplify the potential of this approach for systematic investigation of cellular responses to multiple microenvironmental cues.

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“…Topology has been explored in a controlled manner, principally by using organic polymer materials and techniques such as lithography for 2D and electrospinning for 3D materials, both of which allow fine control of the structure on the micrometer to nano scale. 107 A broad range of materials encompassing most major classes of plastic, including biodegradable polymers such as polycaprolactone, have now been examined. 10 Responses over scales of a few nanometers (surface roughness) to hundreds of micrometers have been explored, and the effect of feature shape, size, orientation, and density within these range of scales, Figure 4, have been considered.…”

Section: Tissue Culture Surfaces As Tools Tomentioning

confidence: 99%

Section: The Influence Of Topology: Early Observations Identified Thementioning

confidence: 99%

The Importance and Clinical Relevance of Surfaces in Tissue Culture

Hickman

Boocock

Pockley

et al. 2016

ACS Biomater. Sci. Eng.

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Cell and tissue culture has evolved from the use of simple glassware for the propagation of cells and tissues into a comprehensive platform for interrogating complex biological systems, directing cell fate and deriving products with clinical and therapeutic value.However, despite significant advances, current in vitro culture approaches remain limited in their capacity to model the clinical/biological complexities of disease, in part at least due to the deficiencies of existing culture materials. The challenge is therefore to identify innovative materials-based solutions that have greater control over cells in vitro, while better representing biological systems in vivo. Such platforms would be suitable for biomarker discovery and tissue engineering applications. This review examines the development of tissue culture materials, advances in our understanding of cell-surface interactions and the application of this knowledge towards the development of new approaches for better examining biological events.3 he ability to culture cells and tissues in vitro is a fundamental aspect of modern science. Established early in the twentieth century, notably through the work of Harrison R.G. of John Hopkins University 1 , the ability to culture cells and tissues has markedly improved during the intervening period. The field has progressed from an ability to maintain and culture tissue for extended periods, through the discovery and establishment of immortal cell lines, to today, where tissue engineering is making considerable progress in the production of artificial tissues and organs in vitro [2][3][4] . Key to these successes have been advances in the culture surfaces on which cells and tissues are grown.This review summarizes progress in the development of tissue culture materials, highlights current requirements and existing limitations for in vitro culture, and examines their relevance to clinical questions and our current understanding of tissue culture materials design. Although long-established, current culture materials may not always be appropriate for modelling in vivo conditions, and innovative strategies are therefore required in order to overcome existing limitations. Current Issues with Tissue Culture:Numerous articles have highlighted the drawbacks and limitations of current in vitro culture systems 5,6 . Concerns revolve around deficiencies in the culture systems and the tissue they generate. Although the latter can be linked to the quality of the initial cellular material, contamination and/or poor maintenance of historical cell lines 6,7 , it can also result from deficiencies in the culture systems i.e. not all cell populations are amenable to in vitro culture.Problems are compounded once tissue enters in vitro culture, as derived populations are expected to maintain their in vivo relevance. However, cells naturally adapt to the local environment and T 4 prolonged culture of immortalized cell lines results in a progressive divergence from the parental population 6,8,9 . Although loss or gain of ab...

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Nanopatterned Surfaces for Biomedical Applications

Cited by 12 publications

References 64 publications

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

Protein Micropatterning in 2.5D: An Approach to Investigate Cellular Responses in Multi-Cue Environments

The Importance and Clinical Relevance of Surfaces in Tissue Culture

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