Nanopipettes: probes for local sample analysis

Saha-Shah, Anumita; Weber, Anita L.; Karty, Jonathan A.; Ray, Steven J.; Hieftje, Gary M.; Baker, Lane A.

doi:10.1039/c5sc00668f

Cited by 50 publications

(55 citation statements)

References 45 publications

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“…Them etabolites in cytoplasm have been characterized as shown in Figure 5 (signals labeled with ar ed star). [83] These findings are promising for the application of nanopipettes to the investigation of sample heterogeneity and population analysis.…”

Section: Other Advanced Applications Of Nanopipettesmentioning

confidence: 92%

Confined Nanopipette Sensing: From Single Molecules, Single Nanoparticles, to Single Cells

et al. 2018

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Nanopipette provides a promising confined space, which exhibits advances in electrochemical, optical and mass spectrometric measurements at the nanoscale. It has been employed to reveal the hidden population properties and dynamics of single molecules and single particles. Moreover, new detection mechanisms developed on nanopipette lead to the discovery of detailed single cell information at high spatial and high temporal resolution. Herein, we focus on the fabrication and characterization of nanopipettes, summarize their wide applications for single entity analysis, and conclude with an outlook for advanced practical sensing.

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Section: Other Advanced Applications Of Nanopipettesmentioning

confidence: 92%

Confined Nanopipette Sensing: From Single Molecules, Single Nanoparticles, to Single Cells

et al. 2018

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Section: Methods Based On Scanning Ion Conductance Microscopymentioning

confidence: 99%

“…[40] To demonstrate longitudinal sampling, the authors employed enzyme-linked immunosorbent assay (ELISA) to track the Small Methods 2018, 2, 1700300 Adapted with permission. [16] Copyright 2015, Royal Society of Chemistry. c-e) Adapted with permission.…”

Section: Nanostrawsmentioning

confidence: 99%

“…The development of these methods is driving the further refinement of sample‐preparation methods for downstream analysis of the extracted content. Next‐generation sequencing has now been applied to sequence just a fraction of a cell's RNA, and the extracted contents also can be analyzed by electron microscopy or advanced mass‐spectrometry techniques …”

Section: Introductionmentioning

confidence: 99%

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Sampling from Single Cells

Actis

2018

Small Methods

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The cell is the fundamental unit of biology. Major methodological advances in engineering and molecular biology have enabled the 'omics analysis of individual cells and supported biologists in understanding the deepest difference between health and disease. These advancements were based on the assumption that a single cell needs to be lysed or fixed before any in‐depth analysis can be performed. Here, a picture is painted of the innovative methods used for extracting the contents of living cells without affecting their viability. These novel methods are now empowering the biological community to repeatedly interrogate a single cell over time, thus giving a dynamic representation of the cell's 'omics rather than a snapshot at a particular time point.

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“…235 Moreover, the use of nanopipettes to directly sample native cellular environments and then evaluate local distribution of biomolecules such as mRNA, DNA, and lipids has been reported. 236–238 …”

Section: Solid-state Nanoporesmentioning

confidence: 99%