Nanoplastic production procedure for scientific purposes: PP, PVC, PE-LD, PE-HD, and PS

Merdy, Patricia; Delpy, Floriane; Bonneau, Adrien; Villain, S.; Iordachescu, Lucian; Vollertsen, Jes; Lucas, Yves

doi:10.1016/j.heliyon.2023.e18387

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…Several methods have been described for model NPs' manufacturing of different chemical types for the purposes of method development and validation, as well as in vitro toxicity and risk assessment testing [3]. Particularly challenging is achieving the highquality, low batch-to-batch variation, and uniform size (ideally monodisperse) of the NPs produced.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Nanoprecipitated PET Nanoplastics by <sup>1</sup>H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Djapovic,

Apostolovic,

Postic

et al. 2023

Preprint

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Manufactured nanoplastic particles (NPs) are indispensable for in vitro and in vivo testing and health risk assessment of this emerging environmental contaminant. High surface area and inherent hydrophobicity of plastic material, makes production of NPs devoid of any contaminants very challenging. In this study we produced nanoprecipitated polyethylene terephthalate (PET) NPs (300 nm hydrodynamic diameter) and characterized presence of ionic surfactant sodium dodecyl sulfate (SDS) by 1H NMR. Relative ratio of NP/surfactant was monitored on the basis of chemical shifts characteristic for PET and SDS. For a wide range of surfactant/NP ratio (1:17 to 1:1.2), measured zeta potential changed from -42.10 to -34.93 mV, but no clear differences were observed in cellular assays performed in protein-rich media on primary human cells. The remaining impurities contributed to the outcome of biological assays applied in protein-free buffers, such as human red blood cells hemolysis. As size, shape, zeta potential and contaminants of NPs may all be relevant parameters for biological effects of NPs, relative quantification of impurities exemplified in our work by application of 1H NMR for PET NPs and ionic surfactant SDS could be a valuable auxiliary method in quality control of manufactured NPs.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of Nanoprecipitated PET Nanoplastics by <sup>1</sup>H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Djapovic,

Apostolovic,

Postic

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Several methods have been described for model NPs’ manufacturing of different chemical types for the purposes of method development and validation, as well as in vitro toxicity and risk assessment testing [ 3 ]. Particularly challenging is achieving the high-quality, low batch-to-batch variation, and uniform size (ideally monodisperse) of the NPs produced.…”

Section: Introductionmentioning

confidence: 99%

“…Heinlaan et al [ 9 ] have clearly shown that the observed toxicity of commercial PS-NPs was induced by toxic water-soluble additives (e.g., antimicrobial NaN 3 and surfactants) in the PS-NPs preparations, as revealed by the disappearance of toxicity after particle dialysis. Therefore, for studying the biological effects of NPs more and more, researchers produce and characterize their own NP material, and in the last several years, there has been an increasing number of publications reporting the preparation of NPs [ 3 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…The “bottom-up” approach stems from traditional colloidal chemistry methods such as conventional dispersed media (emulsions and nanoprecipitation), where ionic or non-ionic surfactants enable NPs dispersibility [ 8 ]. However, even after extensive NP rinsing, the residual traces of surfactants are difficult to remove [ 3 ]. The recent critical review highlighted the importance of the purification of PS-NP suspensions to remove additives, such as surfactants, before performing toxicity evaluations [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Djapovic,

Apostolovic,

Postic

et al. 2023

Polymers

View full text Add to dashboard Cite

Manufactured nanoplastic particles (NPs) are indispensable for in vitro and in vivo testing and a health risk assessment of this emerging environmental contaminant is needed. The high surface area and inherent hydrophobicity of plastic materials makes the production of NPs devoid of any contaminants very challenging. In this study, we produced nanoprecipitated polyethylene terephthalate (PET) NPs (300 nm hydrodynamic diameter) with an overall yield of 0.76%. The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. For a wide range of surfactant/NP ratios (17:100 to 1.2:100), the measured zeta potential changed from −42.10 to −34.93 mV, but with an NP concentration up to 100 μg/mL, no clear differences were observed in the cellular assays performed in protein-rich media on primary human cells. The remaining impurities contributed to the outcome of the biological assays applied in protein-free buffers, such as human red blood cell hemolysis. The presence of SDS increased the NP-induced hemolysis by 1.5% in protein-rich buffer and by 7.5% in protein-free buffer. As the size, shape, zeta potential, and contaminants of NPs may all be relevant parameters for the biological effects of NPs, the relative quantification of impurities exemplified in our work by the application of 1H NMR for PET NPs and the ionic surfactant SDS could be a valuable auxiliary method in the quality control of manufactured NPs.

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Neurotoxic effects of polystyrene nanoplastics on memory and microglial activation: Insights from in vivo and in vitro studies

Paing,

Eom,

Song

et al. 2024

Science of The Total Environment

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Nanoplastic production procedure for scientific purposes: PP, PVC, PE-LD, PE-HD, and PS

Cited by 5 publications

References 30 publications

Characterization of Nanoprecipitated PET Nanoplastics by <sup>1</sup>H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Characterization of Nanoprecipitated PET Nanoplastics by <sup>1</sup>H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Neurotoxic effects of polystyrene nanoplastics on memory and microglial activation: Insights from in vivo and in vitro studies

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