Nanoplastic sizes and numbers: quantification by single particle tracking

Molenaar, Robert; Chatterjee, Swarupa; Kamphuis, B.; Segers-Nolten, Ine; Claessens, Mireille Maria Anna Elisabeth; Blum, Christian

doi:10.1039/d0en00951b

Cited by 48 publications

(33 citation statements)

References 25 publications

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“…However, as the amount of plastic garbage generated increases, NP contamination in the environment may have an impact on the human community, as a whole. Recent studies have indicated the release of approximately 11.6 billion MPs and 3.1 billion NPs from a single plastic tea bag [ 42 ], at least 300 billion NPs/g from face scrubs [ 43 ], billions of NPs/mL (average size 160 nm) of water from polystyrene disposable cups [ 44 ], and an exposure of 14,600–4,550,000 MPs per capita per day from polypropylene infant feeding bottles [ 45 ], which are considered common sources of exposure to humans. The number of particles used for exposure in this study corresponds closely to the natural exposure of NPs in the human system; however, looking at the continuous exposure of NPs in the natural environment, these NPs may cause even more damage to human health than observed in the present study.…”

Section: Discussionmentioning

confidence: 99%

The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes

Sarma¹,

Dubey

Samarth

et al. 2022

Nanomaterials

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Environmental exposure to microplastics (MPs) and nanoplastics (NPs) is an increasing concern from human health perspectives. Little information on the genotoxic and cytotoxic potential of NP particles in human cells is available. We aimed to assess the cytotoxic and genotoxic potential of polystyrene nanoplastics (PSNPs) at different concentrations (2000μg/mL, 1000μg/mL, and 500μg/mL) by using chromosomal aberration (CA) and cytokinesis-block micronucleus assays (CBMN) on human peripheral lymphocytes. Dose-dependent hemolytic activity and cell viability were observed against the PSNPs exposure. Increased chromosomal aberrations, such as chromosomal breaks and dicentric chromosomes, and an increase in nucleoplasmic bridge (NBP) formation and nuclear budding (NBUD) were observed. The frequency of mitotic index (MI) decreased significantly in the PSNP-exposed groups from lower to higher concentrations. A significant increase in micronuclei (MN) formation and cytostasis% and a dose-dependent reduction in nuclear division index (NDI) in PSNP-exposed groups indicated oxidative stress-mediated cytotoxicity, DNA damage, and genomic instabilities due to PSNP exposure in human lymphocyte cells. This study highlights the importance of understanding the toxic mechanisms and associated chronic and acute health effects on humans due to exposure to this pervasive environmental pollutant.

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Section: Discussionmentioning

confidence: 99%

The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes

Sarma¹,

Dubey

Samarth

et al. 2022

Nanomaterials

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“…[ 54 ] Otherwise, nanoparticle tracking analysis (NTA) consents imaging nanoplastics by recording the diffracted light from an incident laser and furnishes information on their size distribution, concentration, and Brownian diffusion. [ 55 ] It has been employed to examine the temporal fragmentation of disposable polystyrene (PS) coffee cup lid into nanoplastics. [ 56 ] NTA shows potential in exploring the nanoplastic removal ability of nano/microrobots.…”

Section: Measurement Techniques For Nano/microplastics Degradationmentioning

confidence: 99%

Nano/Microplastics Capture and Degradation by Autonomous Nano/Microrobots: A Perspective

Urso

Pumera

2022

Adv Funct Materials

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The growing use of plastic materials has led to the continuous accumulation of wastes in marine environments, which fragment into hazardous micro‐and nanoplastics. These plastic particles absorb toxic organic pollutants on their surface, support bacterial biofilms growth, and propagate through the food chain, posing serious risks for human health. Therefore, nano/microplastics pollution has become a global issue, making their definitive elimination compulsory. Self‐propelled nano/microrobots have demonstrated efficient removal of nano/microplastics from water, combining enhanced physicochemical properties of nano/microscale materials and active motion. During the last year, the potential of this technology to degrade nano/microplastics has been investigated. Here, the most advanced strategies for nano/microplastics capture and subsequent degradation by autonomous nano/microrobots are critically reviewed. A short introduction to the main propulsion mechanisms and experimental techniques for studying nano/microplastics degradation is also provided. Forthcoming challenges in this research field are discussed proactively. This perspective inspires future nano/microrobotic designs and approaches for water purification from nano/microplastics and other emerging pollutants.

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“…Another technique that can analyse stained plastics coupled with fluorescence microscopy is the single‐particle tracking, which allows the identification of the number and the size of nanoparticles. For example, Molenaar et al [86] . combined these two techniques to analyse commercially available PS beads with a nominal diameter of 400 nm, 200 nm, 100 nm and 40 nm and plastic particles obtained by grinding a commercially available disposable PS plastic cup into a powder with a commercial kitchen mixer.…”

Section: Stainingmentioning

confidence: 99%

Photoluminescence‐Based Techniques for the Detection of Micro‐ and Nanoplastics

Capolungo

Genovese

Montalti

et al. 2021

Chemistry A European J

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The growing numbers related to plastic pollution are impressive, with ca. 70 % of produced plastic (>350 tonnes/year) being indiscriminately wasted in the environment. The most dangerous forms of plastic pollution for biota and human health are micro‐ and nano‐plastics (MNPs), which are ubiquitous and more bioavailable. Their elimination is extremely difficult, but the first challenge is their detection since existing protocols are unsatisfactory for microplastics and mostly absent for nanoplastics. After a discussion of the state of the art for MNPs detection, we specifically revise the techniques based on photoluminescence that represent very promising solutions for this problem. In this context, Nile Red staining is the most used strategy and we show here its pros and limitations, but we also discuss other more recent approaches, such as the use of fluorogenic probes based on perylene‐bisimide and on fluorogenic hyaluronan nanogels, with the added values of biocompatibility and water solubility.

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Nanoplastic sizes and numbers: quantification by single particle tracking

Abstract: Easy method to count and size plastic nanoparticles in water. A combination of sensitive fluorescence video microscopy, NileRed staining of plastic particles, and single particle tracking allows for counting and sizing nanoplastics.

Cited by 48 publications

References 25 publications

The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes

The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes

Nano/Microplastics Capture and Degradation by Autonomous Nano/Microrobots: A Perspective

Photoluminescence‐Based Techniques for the Detection of Micro‐ and Nanoplastics

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