Nanoplastics induce molecular toxicity in earthworm: Integrated multi-omics, morphological, and intestinal microorganism analyses

Tang, Ronggui; Zhu, Dong; Luo, Yongming; He, Defu; Zhang, Haibo; EI-Naggar, Ali; Palansooriya, Kumuduni Niroshika; Chen, Keyi; Yan, Yan; Lu, Xinghang; Ying, Minshen; Sun, Tao; Cao, Yuntao; Diao, Zhihan; Zhang, Yuxin; Lian, Yichen; Chang, Scott X.; Cai, Yanjiang

doi:10.1016/j.jhazmat.2022.130034

Cited by 47 publications

(5 citation statements)

References 80 publications

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“…This indicated that exposure to the photoaged TWP may further lead to damage at the tissue level. Previous studies have also reported that microplastics can easily harm keratinocytes in epithelial tissues such as the body wall and intestines. , Overall, it is necessary to further characterize the oxidative stress and damage caused by the original and photoaged TWP.…”

Section: Results and Discussionmentioning

confidence: 99%

Photoaged Tire Wear Particles Leading to the Oxidative Damage on Earthworms (Eisenia fetida) by Disrupting the Antioxidant Defense System: The Definitive Role of Environmental Free Radicals

Chen,

Liu,

Yang

et al. 2024

Environ. Sci. Technol.

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Tire wear particles (TWPs) have caused increasing concerns due to their detrimental effects on the soil ecosystem. However, the role of weathering in altering the toxicity of TWP to soil organisms is poorly understood. In this study, the toxicity of original and photoaged TWP was compared using earthworms (Eisenia fetida) as soil model organisms. The obtained results indicated that photoaging of TWP resulted in an increase of environmentally persistent free radicals (EPFRs) from 3.69 × 10 17 to 5.20 × 10 17 spin/g. Meanwhile, photoaged TWP induced the changes of toxic endpoint in E. fetide, i.e., the increase of the weight loss and death ratio from 0.0425 to 0.0756 g/worm and 23.3 to 50% compared to original TWP under a 10% concentration, respectively. Analyses of transcriptomics, antioxidant enzyme activity, and histopathology demonstrated that the enhanced toxicity was mainly due to oxidative damage, which was induced by disruption in the antioxidant defense system. Free-radical quenching and correlation analysis further suggested that the excessive production of ex vivo reactive oxygen species, induced by EPFRs, led to the exhaustion of the antioxidant defense system. Overall, this work provides new insights into the potential hazard of the weathered TWP in a soil environment and has significant implications for the recycling and proper disposal of spent tire particles.

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Section: Results and Discussionmentioning

confidence: 99%

Photoaged Tire Wear Particles Leading to the Oxidative Damage on Earthworms (Eisenia fetida) by Disrupting the Antioxidant Defense System: The Definitive Role of Environmental Free Radicals

Chen,

Liu,

Yang

et al. 2024

Environ. Sci. Technol.

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“…Thus, when two types of MPs (PS and car tire abrasion) were present in the soil, changes in the activity of multiple biomarkers in Eisenia fetida could be detected, but most enzymatic activities recovered after 28 d [173]. However, Tang et al [174,175] recently informed of the relevant effects of PS (25-30 µm and 90-110 nm) on E. fetida, such as damage to digestive and immune systems, reduction in gut microorganisms' diversity and species richness or disturbance of the osmoregulatory metabolism.…”

Section: Terrestrial Ecosystemsmentioning

confidence: 99%

Interactions of Microplastics with Pesticides in Soils and Their Ecotoxicological Implications

2023

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In the middle of the 20th century, the production of plastics exploded worldwide because of their low cost and the versatility of their applications. However, since plastic debris is highly resistant to environmental degradation, a growing presence of plastics in all the ecosystems has been confirmed. Among them, plastic particles < 5 mm, also known as microplastics (MPs), are of special concern because they are dispersed in aerial, terrestrial and aquatic environments, being the soil the main environmental sink of these contaminants. Due to their large specific surface area and hydrophobicity, MPs are considered good adsorbents for other environmental organic pollutants also present in terrestrial ecosystems, such as pharmaceuticals, personal-care products or pesticides with which they can interact and thus modify their environmental fate. In this review article, we examine the recent literature (from 2017 to 2022) to get a better understanding of the environmental fate of pesticides in soil (adsorption, mobility and/or degradation) when they are simultaneously present with MPs and the ecological risks on living organisms of the interactions between MPs and pesticides in soil. More studies are needed to fully understand the toxicological impact of the copresence in soil of pesticides and MPs.

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“…Conversely, it is only just detected in the dead worm (cross peaks barely discernible from the noise, see inset Figure 2A) suggesting it is either used up as the worm dies or rapidly breaks down after death. Considering HEFS levels have been correlated to various stressors including, plant polyphenols, 42 Hg, 43 nanoplastics, 44 and lead, 45 its high abundance in vivo suggests it represents a key indicator for understanding stress processes within living earthworms in future studies. While the HSQC was successful in detecting a range of metabolites, a couple of considerations are warranted.…”

Section: Analyticalmentioning

confidence: 99%

Development of a Simple Cost Effective Oxygenation System for In Vivo Solution State NMR in 10 mm NMR Tubes

Costa,

Lysak,

Soong

et al. 2024

Anal. Chem.

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In vivo NMR is evolving into an important tool to understand biological processes and environmental responses. Current approaches use flow systems to sustain the organisms with oxygenated water and food (e.g., algae) inside the NMR. However, such systems have the potential to leak and clog (potentially damaging costly hardware), require large volumes of media, and multiple expensive HPLC pumps. The proposed "oxygenation system", uses a simple "double slit" adapter and a single air/oxygen flow line into the NMR. The design is especially suited to larger diameter probes given that standard flow systems would require higher flow rates thus amplifying the potential and impact of leaks/clogs. Traditionally, in vivo NMR of small organisms (e.g., Daphnia) have required 2D NMR in combination with 13 C enrichment to overcome susceptibility distortions and provide information rich metabolic profiles. Here Daphnia magna, Eisenia fetida and Artemia franciscana are used to demonstrate the potential of the oxygenation system. Survivability tests and 1 H time-resolved monitoring were first performed on D. magna, while E. fetida contained enough biomass to permit 1 H− 13 C HSQC, 13 C− 1 H HETCOR and 31 P NMR without isotopic enrichment. Finally, STOCSY of 1D 13 C NMR was used to follow the growth of A. franciscana (without 13 C enrichment) for 48 h after birth, which helps visualize trends across a series of 1D in vivo data. In summary, application of the oxygenation system toward larger diameter probes allows the collection of NMR data without enrichment, offering a promising solution to better understand processes in vivo.

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Nanoplastics induce molecular toxicity in earthworm: Integrated multi-omics, morphological, and intestinal microorganism analyses

Cited by 47 publications

References 80 publications

Photoaged Tire Wear Particles Leading to the Oxidative Damage on Earthworms (Eisenia fetida) by Disrupting the Antioxidant Defense System: The Definitive Role of Environmental Free Radicals

Photoaged Tire Wear Particles Leading to the Oxidative Damage on Earthworms (Eisenia fetida) by Disrupting the Antioxidant Defense System: The Definitive Role of Environmental Free Radicals

Interactions of Microplastics with Pesticides in Soils and Their Ecotoxicological Implications

Development of a Simple Cost Effective Oxygenation System for In Vivo Solution State NMR in 10 mm NMR Tubes

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