Nanopore back titration analysis of dipicolinic acid

Yang, Han; Zhou, Shuo; Wang, Liang; Guan, Xiyun

doi:10.1002/elps.201400255

Cited by 17 publications

(10 citation statements)

References 35 publications

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“…Since analytes pass through the nanopore at one molecule at a time, simultaneous detection and quantification of multiple components in a solution mixture can be readily accomplished using a single nanopore as long as the nanopore itself can provide enough resolution . During the past decade, nanopore technology has shown great potential applications in numerous fields, such as medical diagnosis, − homeland security and biodefense, , pharmaceutical screening, , and environmental protection. , …”

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confidence: 99%

Label-Free Nanopore Single-Molecule Measurement of Trypsin Activity

et al. 2016

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Trypsin is the most important digestive enzyme produced in the pancreas, and is a useful biomarker for pancreatitis. In this work, a rapid and sensitive method for the quantitative determination of trypsin activity is developed by using a biological alpha-hemolysin protein nanopore. Due to its much larger molecular diameter than the narrow pore constriction, trypsin itself cannot transport through the alpha-hemolysin channel. Hence, an indirect trypsin detection method is developed by monitoring its proteolytic cleavage of a lysine-containing peptide substrate. Based on the current modulations produced by the translocation of the substrate degradation products in the nanopore, the activity levels of trypsin could be determined. The method is rapid and highly sensitive, with picomolar concentrations of trypsin detected in minutes. In addition, the effects of cation and temperature on the sensor sensitivity, trypsin inhibition, and serum sample analysis are also investigated.

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confidence: 99%

Label-Free Nanopore Single-Molecule Measurement of Trypsin Activity

et al. 2016

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“…In this work, by mutating the methionine residue of the wild-type α-HL protein at position 113, a series of homoheptamer mutants were constructed and imparted with the capability to recognize cationic, anionic, aromatic, and hydrophobic molecules based on electrostatic, aromatic–aromatic, cation−π, and hydrophobic interactions (Figure ). Note that the β-barrel of the α-HL protein pore has three sensing regions/zones, including the primary (close to the narrowest constriction of the nanopore), secondary (near position 135), and trans-opening. , Position 113 is located in the primary sensing zone and is most widely utilized to design engineered nanopores for various applications. ,− …”

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confidence: 99%

Nanopore Stochastic Sensing Based on Non-covalent Interactions

et al. 2021

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A variety of species could be detected by using nanopores engineered with various recognition sites based upon non-covalent interactions, including electrostatic, aromatic, and hydrophobic interactions. The existence of these engineered non-covalent bonding sites was supported by the single-channel recording technique. The advantage of the non-covalent interaction-based sensing strategy was that the recognition site of the engineered nanopore was not specific for a particular molecule but instead selective for a class of species (e.g., cationic, anionic, aromatic, and hydrophobic). Since different species produce current modulations with quite different signatures represented by amplitude, residence time, and even characteristic voltage-dependence curve, the non-covalent interaction-based nanopore sensor could not only differentiate individual molecules in the same category but also enable differentiation between species with similar structures or molecular weights. Hence, our developed non-covalent interaction-based nanopore sensing strategy may find useful application in the detection of molecules of medical and/or environmental importance.

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“…4−6 Thus, numbers of attempts have been made to explore efficient and fast detection methods. Recently, several techniques for DPA detection have been developed such as gas chromatography/ mass spectrometry, 7 surface-enhanced Raman scattering (SERS), 8−10 electrochemical detection, 1,11 molecular imprinting, 12−14 fluorescence spectroscopy 15,16 and so on. 3,17,18 Traditional detection methods such as gas chromatography/ mass spectrometry usually require complicated and timeconsuming sample preparation processes and relatively expensive instruments.…”

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confidence: 99%

Multiporous Terbium Phosphonate Coordination Polymer Microspheres as Fluorescent Probes for Trace Anthrax Biomarker Detection

Luo

Zhang

et al. 2019

ACS Appl. Mater. Interfaces

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Lanthanide coordination polymers have been recently regarded as attractive sensing materials because of their selectivity, high sensitivity, and rapid response ability. In this research, the multiporous terbium phosphonate coordination polymer microspheres (TbP-CPs) were prepared as a novel fluorescent probe, which showed a fluorescence turn-on response capability for the detection of the trace anthrax biomarker dipicolinate acid (DPA). The morphology and chemical composition of as-prepared TbP-CPs were characterized in detail. The TbP-CPs have the vegetable-flower-like structure and microporous surface. In addition, the as-prepared TbP-CPs not only possess the merits of convenience and simple preparation with high yield but also have the excellent characters as fluorescent probes, such as high stability, good selectivity, and rapid detection ability within 30 s. This proposed sensor could detect DPA with a linear relationship in concentrations ranging from 0 to 8.0 μM and a high detection sensitivity of 5.0 nM. Furthermore, the successful applications of DPA detection in urine and bovine serum were demonstrated. As a result, the recovery ranged from 93.93–101.6%, and the relative standard deviations (RSD) were less than 5%.

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Nanopore back titration analysis of dipicolinic acid

Cited by 17 publications

References 35 publications

Label-Free Nanopore Single-Molecule Measurement of Trypsin Activity

Label-Free Nanopore Single-Molecule Measurement of Trypsin Activity

Nanopore Stochastic Sensing Based on Non-covalent Interactions

Multiporous Terbium Phosphonate Coordination Polymer Microspheres as Fluorescent Probes for Trace Anthrax Biomarker Detection

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