Nanopore R10.4 metagenomic detection of blaCTX-M/blaDHA antimicrobial resistance genes and their genetic environments in stool

Campos-Madueno, Edgar I.; Aldeia, Claudia; Endimiani, Andrea

doi:10.1038/s41467-024-51929-y

Cited by 2 publications

References 59 publications

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Decade of Carbapenemase-Producing Enterobacterales in New Caledonia: Integrative Surveillance Though Genomic, Phenotypic and Clinical approaches

Colot,

Bourles,

Cousin

et al. 2024

Preprint

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Carbapenemase-producing Enterobacterales (CPE) have been identified by the World Health Organization as global priority pathogens. The dissemination of these bacteria and outbreaks within healthcare facilities are of serious concern. This study investigated the transmission patterns and genetic characteristics of CPE isolated in New Caledonia from 2013 to 2022. The isolates were identified and characterized both phenotypically and whole-genome sequencing (WGS). In total 214 CPE were isolated: 199 non duplicate clinical isolates from 164 patients and 15 from hospital environmental surface. The most common genera in clinical samples were Enterobacter (34%) and Klebsiella (25%), with 194 isolates (98%) carrying IMP-type carbapenemase. WGS of 89 isolates revealed the dominance of the blaIMP-4 carbapenemase gene, found in 82 isolates. The blaIMP-4 was primarily predicted to be carried by IncL/M-type plasmid, found in 69% of the sequenced isolates. Our work revealed the circulation of 12 bacterial clusters with 61 strains involved in outbreaks or persistent over time. Genomic, phenotypic and clinical approaches identified 12 distinct outbreaks involving IMP producers. These results highlight the importance of studying plasmid transmission to better prevent silent spread of CPE. Ultimately, this study provides new guidelines for limiting the clinical spread of CPE in New Caledonia.

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Decade of Carbapenemase-Producing Enterobacterales in New Caledonia: Integrative Surveillance Though Genomic, Phenotypic and Clinical approaches

Colot,

Bourles,

Cousin

et al. 2024

Preprint

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Gastrointestinal Microbiota in Gastric Cancer: Potential Mechanisms and Clinical Applications—A Literature Review

Wu,

Tian,

Zou

et al. 2024

Cancers

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Emerging evidence highlights the crucial role of gastrointestinal microbiota in the pathogenesis of gastric cancer. Helicobacter pylori (H. pylori) infection stands out as a primary pathogenic factor. However, interventions such as anti-H. pylori therapy, gastric surgeries, immunotherapy, and chronic inflammation significantly remodel the gastric microbiome, implicating a broader spectrum of microorganisms in cancer development. These microbial populations can modulate gastric carcinogenesis through various mechanisms, including sustained chronic inflammation, bacterial genotoxins, alterations in short-chain fatty acids, elevated gastrointestinal bile acids, impaired mucus barrier function, and increased concentrations of N-nitrosamines and lactic acid. The dynamic changes in gut microbiota also critically influence the outcomes of anti-cancer therapies by modifying drug bioavailability and metabolism, thus affecting therapeutic efficacy and side effect profiles. Additionally, the effectiveness of radiotherapy can be significantly impacted by gut microbiota alterations. Novel therapeutic strategies targeting the microbiome, such as dietary interventions, probiotic and synbiotic supplementation, and fecal microbiota transplantation, are showing promise in cancer treatment. Understanding the intricate relationship between the gut microbiota and gastric cancer is essential for developing new, evidence-based approaches to the prevention and treatment of this malignancy.

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Nanopore R10.4 metagenomic detection of blaCTX-M/blaDHA antimicrobial resistance genes and their genetic environments in stool

Cited by 2 publications

References 59 publications

Decade of Carbapenemase-Producing Enterobacterales in New Caledonia: Integrative Surveillance Though Genomic, Phenotypic and Clinical approaches

Decade of Carbapenemase-Producing Enterobacterales in New Caledonia: Integrative Surveillance Though Genomic, Phenotypic and Clinical approaches

Gastrointestinal Microbiota in Gastric Cancer: Potential Mechanisms and Clinical Applications—A Literature Review

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