Nanoprecipitated catestatin released from pharmacologically active microcarriers (PAMs) exerts pro-survival effects on MSC

Angotti, Carmelina; Venier-Julienne, Marie-Claire; Penna, Cláudia; Femminò, Saveria; Sindji, Laurence; Paniagua, C.; Montero‐Menei, Claudia N.; Pagliaro, Pasquale

doi:10.1016/j.ijpharm.2016.11.050

Cited by 2 publications

(3 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, a study with fibronectin-coated pharmacologically active microcarriers (FN-PAM) examined whether the incorporation of CST into FN-PAM and its release affects the survival of mesenchymal stem cells (MSCs) under hypoxia-generated stress conditions. It was found that this controlled release of CST limited hypoxic MSC death and enhanced cell survival in the post-hypoxic environment [ 74 ]. Hence, it appears that effective CST delivery may be a potent approach for repairing the damage caused by prolonged hypoxia.…”

Section: Chromogranin a Derivative: Catestatinmentioning

confidence: 99%

The Role of Catestatin in Preeclampsia

Bralewska,

Pietrucha,

Sakowicz

2024

IJMS

View full text Add to dashboard Cite

Preeclampsia (PE) is a unique pregnancy disorder affecting women across the world. It is characterized by the new onset of hypertension with coexisting end-organ damage. Although the disease has been known for centuries, its exact pathophysiology and, most importantly, its prevention remain elusive. The basis of its associated molecular changes has been attributed to the placenta and the hormones regulating its function. One such hormone is chromogranin A (CgA). In the placenta, CgA is cleaved to form a variety of biologically active peptides, including catestatin (CST), known inter alia for its vasodilatory effects. Recent studies indicate that the CST protein level is diminished both in patients with hypertension and those with PE. Therefore, the aim of the present paper is to review the most recent and most relevant in vitro, in vivo, and clinical studies to provide an overview of the proposed impact of CST on the molecular processes of PE and to consider the possibilities for future experiments in this area.

show abstract

Section: Chromogranin a Derivative: Catestatinmentioning

confidence: 99%

The Role of Catestatin in Preeclampsia

Bralewska,

Pietrucha,

Sakowicz

2024

IJMS

View full text Add to dashboard Cite

show abstract

“…As Hypoxia/Reoxygenation (H/R) we considered cell survival studied after exposition to DMEM-2% FBS with 3% O 2 and 5% CO 2 for 72-h and a subsequent reoxygenation (21% O 2 and 5% CO 2 ) for 3-h [39][40][41][42]. H/R protocol was obtained in an hypoxic chamber (INVIVO2 200, Belsar, Varese, Italy).…”

Section: Study Of Protection Against Oxidative Stress and Study Of Intracellular Mechanisms Of Cgrp Protection Against Hypoxia/reoxygenatmentioning

confidence: 99%

“…At the end of experiments cell survival was assessed using the cell viability test 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit. The plates were read by a spectrophotomer at 570 nm to obtain values of optical density [39]. All values were normalized to mean value of input control cells and expressed as percentage.…”

Section: Cell Survival Assessmentmentioning

confidence: 99%

Cardioprotective effects of calcitonin gene-related peptide in isolated rat heart and in H9c2 cells via redox signaling

Tullio

Penna

Cabiale

et al. 2017

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

The calcitonin-gene-related-peptide (CGRP) release is coupled to the signaling of Angeli's salt in determining vasodilator effects. However, it is unknown whether CGRP is involved in Angeli's salt cardioprotective effects and which are the mechanisms of protection. We aimed to determine whether CGRP is involved in myocardial protection induced by Angeli's salt. We also analyzed the intracellular signaling pathway activated by CGRP. Isolated rat hearts were pre-treated with Angeli's salt or Angeli's salt plus CGRP, a specific CGRP-receptor antagonist, and subjected to ischemia (30-min) and reperfusion (120-min). Moreover, we studied CGRP-induced protection during oxidative stress (HO) and hypoxia/reoxygenation protocols in H9c2 cardiomyocytes. Cell vitality and mitochondrial membrane potential (ΔYm, MMP) were measured using MTT and JC-1 dyes. Angeli's salt reduced infarct size and ameliorated post-ischemic cardiac function via a CGRP-receptor-dependent mechanism. Pre-treatment with CGRP increased H9c2 survival upon challenging with either HO (redox stress) or hypoxia/reoxygenation (H/R stress). Under these stress conditions, reduction in MMP and cell death were partly prevented by CGRP. These CGRP beneficial effects were blocked by CGRP During H/R stress, pre-treatment with either CGRP-receptor, protein kinase C (PKC) or mitochondrial K channel antagonists, and pre-treatment with an antioxidant (2-mercaptopropionylglycine) blocked the protection mediated by CGRP. In conclusion, CGRP is involved in the cardioprotective effects of Angeli's salt. In H9c2 cardiomyocytes, CGRP elicits PKC-dependent and mitochondrial-K-redox-dependent mechanisms. Hence, CGRP is an important factor in the redox-sensible cardioprotective effects of Angeli's salt.

show abstract

Nanoprecipitated catestatin released from pharmacologically active microcarriers (PAMs) exerts pro-survival effects on MSC

Cited by 2 publications

References 45 publications

The Role of Catestatin in Preeclampsia

The Role of Catestatin in Preeclampsia

Cardioprotective effects of calcitonin gene-related peptide in isolated rat heart and in H9c2 cells via redox signaling

Contact Info

Product

Resources

About