Nanoscale bacteriophage biosensors beyond phage display

Lee, Jong‐Wook; Song, Jangwon; Hwang, Mintai P.; Lee, Kwan H.

doi:10.2147/ijn.s51894

Cited by 57 publications

(42 citation statements)

References 60 publications

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“…In the challenging field of bacteria detection, E. coli in particular, OFGs have been extensively used combined to bacteriophage T4 [63][64][65][66]: phages are organisms recognizing their host by specific receptor molecules on their surface, with high sensitivity and specificity to the bacteria and good thermal stability [81]. For all these reasons and being nontoxic to humans, as well as cheap and fast to produce, they emerged as a possible alternative to antibodies in biosensor development.…”

Section: Ofg-based Biosensing Based On Other Bresmentioning

confidence: 99%

Biosensing with optical fiber gratings

et al. 2017

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Optical fiber gratings (OFGs), especially longperiod gratings (LPGs) and etched or tilted fiber Bragg gratings (FBGs), are playing an increasing role in the chemical and biochemical sensing based on the measurement of a surface refractive index (RI) change through a label-free configuration. In these devices, the electric field evanescent wave at the fiber/surrounding medium interface changes its optical properties (i.e. intensity and wavelength) as a result of the RI variation due to the interaction between a biological recognition layer deposited over the fiber and the analyte under investigation. The use of OFG-based technology platforms takes the advantages of optical fiber peculiarities, which are hardly offered by the other sensing systems, such as compactness, lightness, high compatibility with optoelectronic devices (both sources and detectors), and multiplexing and remote measurement capability as the signal is spectrally modulated. During the last decade, the growing request in practical applications pushed the technology behind the OFG-based sensors over its limits by means of the deposition of thin film overlays, nanocoatings, and nanostructures, in general. Here, we review efforts toward utilizing these nanomaterials as coatings for high-performance and low-detection limit devices. Moreover, we review the recent development in OFG-based biosensing and identify some of the key challenges for practical applications. While high-performance metrics are starting to be achieved experimentally, there are still open questions pertaining to an effective and reliable detection of small molecules, possibly up to single molecule, sensing in vivo and multi-target detection using OFG-based technology platforms.

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Section: Ofg-based Biosensing Based On Other Bresmentioning

confidence: 99%

Biosensing with optical fiber gratings

et al. 2017

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“…Phage technology has been used in abstention of antigen-specific peptides with high specificity and affinity for development of bioassays for the identification of various biomarkers [72]. Phage-based assays have been developed for detect M. tuberculosis in clinical samples and culture, as well as for to identify resistance to anti-tubercular drug rifampicin.…”

Section: Bacteriophage Biosensormentioning

confidence: 99%

Biosensors in Antimicrobial Drug Discovery: Since Biology until Screening Platforms

2015

J Microb Biochem Technol

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Antimicrobial drug resistance is a current public health problem, which is compounded by the misuse of antibiotics in medical practice and the emergence of Multidrug-Resistant (MDR) microorganisms. Therefore it is necessary to develop new anti-infective drugs and implement new methodologies able to establish the Antimicrobial Susceptibility (AST) in field and the point-of-care. In this sense biosensors is a promising technology that can detect MDR strains and small molecules in various samples, these devices have the advantages that can be miniaturized for obtain portability, rapidity, and cost-effectiveness. The aim of this work is to present the applications of biosensors technology in antimicrobial drug discovery, since cell based biosensors and cell culture on chips, considering metabolic interactions of the microbial world and the pharmacological response to be inhibited by compounds with promising activity with the end of design antimicrobial drug screening platforms robust, automatable and reproducible

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“…25 The viral capsid is aligned along the shaft and is composed of 2,700 copies of pVIII and ~5 copies of minor coat proteins pIII, pVI, pIX, and pVII located at either end. 21,22 The 50-residue pVIII (98% by mass) is composed of three distinct domains, namely, a negatively charged hydrophilic N-terminal domain (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), an intermediate hydrophobic domain (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39), and a positively charged domain (40)(41)(42)(43)(44)(45)(46)…”

Section: M13 Phagementioning

confidence: 99%

“…3 These recognition elements have been used to design unprecedented materials such as template-synthesized organic-inorganic composite materials 4,5 and sensory materials. 6,7 The viral particles can self-assemble into various ordered structures with well-defined filamentous shapes, which can lead to novel materials for various functional applications, including energy generation, biosensors, [11][12][13][14] semiconductors, 4,5 and tissue-regenerating materials. 13,[15][16][17][18] In addition, there is growing interest in M13 phage as a model system for soft condensed physics 19 and as a biomimetic building block for structured functional materials.…”

Section: Introductionmentioning

confidence: 99%

Chemical modulation of M13 bacteriophage and its functional opportunities for nanomedicine

Chung¹,

Lee²,

Yoo³

2014

IJN

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M13 bacteriophage (phage) has emerged as an attractive bionanomaterial owing to its genetically tunable surface chemistry and its potential to self-assemble into hierarchical structures. Furthermore, because of its unique nanoscopic structure, phage has been proposed as a model system in soft condensed physics and as a biomimetic building block for structured functional materials. Genetic engineering of phage provides great opportunities to develop novel nanomaterials with functional surface peptide motifs; however, this biological approach is generally limited to peptides containing the 20 natural amino acids. To extend the scope of phage applications, strategies involving chemical modification have been employed to incorporate a wider range of functional groups, including synthetic chemical compounds. In this review, we introduce the design of chemoselective phage functionalization and discuss how such a strategy is combined with genetic engineering for a variety of medical applications, as reported in recent literature.

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Nanoscale bacteriophage biosensors beyond phage display

Cited by 57 publications

References 60 publications

Biosensing with optical fiber gratings

Biosensing with optical fiber gratings

Biosensors in Antimicrobial Drug Discovery: Since Biology until Screening Platforms

Chemical modulation of M13 bacteriophage and its functional opportunities for nanomedicine

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Nanoscale bacteriophage biosensors beyond phage display

Cited by 57 publications

References 60 publications

Biosensing with optical fiber gratings

Biosensing with optical fiber gratings

Biosensors in Antimicrobial Drug Discovery: Since Biology until Screening Platforms

Chemical modulation of M13 bacteriophage and&nbsp;its functional opportunities for nanomedicine

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Chemical modulation of M13 bacteriophage and its functional opportunities for nanomedicine