Nanoscale Engineering of Biomaterial Surfaces

Lipski, Anna Marie; Jaquiéry, Claude; Choi, Hoon; Eberli, Daniel; Stevens, Molly M.; Martin, Ivan; Chen, I‐Wei; Shastri, V. Prasad

doi:10.1002/adma.200502617

Cited by 67 publications

(59 citation statements)

References 23 publications

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“…Past studies showed that stochastic nanoroughness altered the organization of focal adhesion complexes in highly migratory preosteoblasts and endothelial cells (24). Because neurons have limited migratory capacity (28), a critical open question was how neurons perceive nanoroughness.…”

Section: Significancementioning

confidence: 99%

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Stochastic nanoroughness modulates neuron–astrocyte interactions and function via mechanosensing cation channels

Blumenthal¹,

Hermanson

Heimrich

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

139

135

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Extracellular soluble signals are known to play a critical role in maintaining neuronal function and homeostasis in the CNS. However, the CNS is also composed of extracellular matrix macromolecules and glia support cells, and the contribution of the physical attributes of these components in maintenance and regulation of neuronal function is not well understood. Because these components possess well-defined topography, we theorize a role for topography in neuronal development and we demonstrate that survival and function of hippocampal neurons and differentiation of telencephalic neural stem cells is modulated by nanoroughness. At roughnesses corresponding to that of healthy astrocytes, hippocampal neurons dissociated and survived independent from astrocytes and showed superior functional traits (increased polarity and calcium flux). Furthermore, telencephalic neural stem cells differentiated into neurons even under exogenous signals that favor astrocytic differentiation. The decoupling of neurons from astrocytes seemed to be triggered by changes to astrocyte apical-surface topography in response to nanoroughness. Blocking signaling through mechanosensing cation channels using GsMTx4 negated the ability of neurons to sense the nanoroughness and promoted decoupling of neurons from astrocytes, thus providing direct evidence for the role of nanotopography in neuron-astrocyte interactions. We extrapolate the role of topography to neurodegenerative conditions and show that regions of amyloid plaque buildup in brain tissue of Alzheimer's patients are accompanied by detrimental changes in tissue roughness. These findings suggest a role for astrocyte and ECM-induced topographical changes in neuronal pathologies and provide new insights for developing therapeutic targets and engineering of neural biomaterials. mechanotransduction | stretch-activated channels | FAM38A | Piezo-1 | polarization

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Section: Significancementioning

confidence: 99%

“…Because macromolecules are in a state of high entropy, and entropy is a statistical measure of randomness, the roughness presented by macromolecules is expected to be stochastic (random). We simulated random ECM nanoroughness using an assembly of monodispersed silica colloids of increasing size (10,24) (Fig. 1A).…”

Section: Significancementioning

confidence: 99%

Stochastic nanoroughness modulates neuron–astrocyte interactions and function via mechanosensing cation channels

Blumenthal¹,

Hermanson

Heimrich

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

139

135

View full text Add to dashboard Cite

show abstract

“…[9,13,14] Work in this area has been facilitated by recent advances in technologies used to modify and observe surfaces with nanostructural features. [9] Nanostructural features for biological interfaces can be created by various techniques such as photolithography, [12] deposition of particles, [15] polymer demixing, [9,16] and metal evaporation. [6] These approaches can yield uniform arrays of various types, including troughs, grooves, and protrusions with nanometerscale dimensions.…”

mentioning

confidence: 99%

Surface Nanopatterning to Control Cell Growth

Richert¹,

Yi²,

Zalzal³

et al. 2008

Advanced Materials

169

124

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“…Since the ECM is essentially a complex matrix composed of polymers, it is not surprising that it possesses both texture and defined mechanical properties. In recent years, substrate stiffness (matrix mechanics) [21] and topography [22,23] have emerged as a very important exogenous signal which in some situations can dwarf the effects of soluble signals, in the lineagespecific differentiation of pluripotent cells. Mechanobiology [24], the coupling of matrix mechanics with cellular signaling, offers opportunities to identify new paradigms for controlling signal networks and is expected to play an important role in the design of "smart" biomaterials.…”

Section: Regenerative Medicinementioning

confidence: 99%

Delivering regeneration

Shastri

2012

Drug Deliv. and Transl. Res.

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Nanoscale Engineering of Biomaterial Surfaces

Cited by 67 publications

References 23 publications

Stochastic nanoroughness modulates neuron–astrocyte interactions and function via mechanosensing cation channels

Stochastic nanoroughness modulates neuron–astrocyte interactions and function via mechanosensing cation channels

Surface Nanopatterning to Control Cell Growth

Delivering regeneration

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