Nanoscale Infrared Spectroscopy of Biological Systems

Abstract

with high affinity and exhibit a robust open probability~0.4. Substituting dual alanines for the 'IQ' residues in the IQ element significantly weakens apocalmodulin binding, and markedly diminishes peak open probability (~0.1). Overexpressing apocalmodulin fully rescues open probability, demonstrating that the effects on opening reflect apocalmodulin binding per se. This extensive structural and functional similarity substantiates a striking conservation of calmodulin regulation across Nav and Cav channels, joint investigation of which now presents as a genuinely synergistic endeavor.

Hybrid AFM for Nanoscale Physicochemical Characterization: Recent Development and Emerging Applications

“…As shown in Figure , by matching the repetition frequency of the mid‐infrared QCL pulses with the resonant frequency of the AFM cantilever, the mechanical resonant enhancement of the cantilever deflection amplitude is achieved. This increases the detection sensitivity of the force on AFM tips due to the thermal expansion and increases the IR sensitivity by several orders of magnitude …”