Nanoscale simulation of the thylakoid membrane response to extreme temperatures

Kulke, Martin; Weraduwage, Sarathi M.; Sharkey, Thomas D.; Vermaas, Josh V.

doi:10.1111/pce.14609

Cited by 5 publications

(4 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent simulations of lipid-only thylakoid membranes have shown an approximate bilayer thickness of ∼3.0 nm. , However, microscopy analyses of dark-adapted plant thylakoids have estimated the thylakoid grana bilayer thickness at ∼4–4.5 nm, ,, in agreement with this study (Figure F–I). Therefore, it is likely that the observed in vivo membrane thickness depends not on the lipid phase but the proteinaceous phase of the thylakoid.…”

Section: Resultssupporting

confidence: 89%

Hydrophobic Mismatch in the Thylakoid Membrane Regulates Photosynthetic Light Harvesting

Wilson,

Clarke,

Carbajal

et al. 2024

J. Am. Chem. Soc.

View full text Add to dashboard Cite

The ability to harvest light effectively in a changing environment is necessary to ensure efficient photosynthesis and crop growth. One mechanism, known as qE, protects photosystem II (PSII) and regulates electron transfer through the harmless dissipation of excess absorbed photons as heat. This process involves reversible clustering of the major light-harvesting complexes of PSII (LHCII) in the thylakoid membrane and relies upon the ΔpH gradient and the allosteric modulator protein PsbS. To date, the exact role of PsbS in the qE mechanism has remained elusive. Here, we show that PsbS induces hydrophobic mismatch in the thylakoid membrane through dynamic rearrangement of lipids around LHCII leading to observed membrane thinning. We found that upon illumination, the thylakoid membrane reversibly shrinks from around 4.3 to 3.2 nm, without PsbS, this response is eliminated. Furthermore, we show that the lipid digalactosyldiacylglycerol (DGDG) is repelled from the LHCII-PsbS complex due to an increase in both the pK a of lumenal residues and in the dipole moment of LHCII, which allows for further conformational change and clustering in the membrane. Our results suggest a mechanistic role for PsbS as a facilitator of a hydrophobic mismatch-mediated phase transition between LHCII-PsbS and its environment. This could act as the driving force to sort LHCII into photoprotective nanodomains in the thylakoid membrane. This work shows an example of the key role of the hydrophobic mismatch process in regulating membrane protein function in plants.

show abstract

Section: Resultssupporting

confidence: 89%

Hydrophobic Mismatch in the Thylakoid Membrane Regulates Photosynthetic Light Harvesting

Wilson,

Clarke,

Carbajal

et al. 2024

J. Am. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…Thus higher N fertilizer application may alleviate negative effect of elevated temperature on photosynthesis [69]. Heat stress can also damage the permeability of the thylakoid membrane and affect photosynthesis [70]. This results in decreased chlorophyll content in plant [50].…”

Section: Discussionmentioning

confidence: 99%

Influence of Elevated CO2 and Temperature on Yield Attributes of Rice and Wheat in Central India

Parmar,

Gupta,

Kollah

et al. 2024

J. Exp. Agric. Int.

View full text Add to dashboard Cite

Elevated CO2 and rising temperature are the major climate changing drivers to attain sustainability in agriculture. The current experiment was carried out to evaluate the extent of impact of climate changing factors on rice and wheat crop. Field experiment was carried out under a Free Air CO2 Enrichment (FACE) condition. The treatments consisted of ambient CO2 and temperature, elevated CO2 of 600ppm + ambient temperature, ambient CO2 + elevated temperature (+2°C), ambient CO2+elevated temperature (+3°C), elevated CO2 of 600 ppm + elevated temperature (+2°C) and elevated CO2 600ppm + elevated temperature (+3°C). The plant yield attributes were biomass, tillers per plant, productive tillers per plant, failure tillers per plant, total number of grains per panicle, failure grains per panicle and test weight. Elevated CO2 stimulated biomass and other yield attributes in both crops. However, elevated temperature inhibited yield attributes. Elevated CO2 enhanced biomass but elevated temperature reduced biomass of crops. Elevated CO2 influenced tillers per plant in rice by -26.9% to 16.6% and in wheat by 3.7% to 25.9%. Elevated temperature affected number tillers up to 26.9%. Number of productive tillers in rice ranged from -28.5% to 21.4% and in wheat by -29.1% to 36%. In wheat the number of grains per panicle varied from -24.3% to 6%. Test weight of grains varied from -9.1% to 4.6% in rice. Combined effect of elevated CO2 and elevated temperature had differential effect on crops. Study highlights that yield attributes and productivity of rice and wheat crop will be affected under elevated CO2 and rising temperature in central part of India and warrants necessary interventions to alleviate climate stress.

show abstract

“…Photosynthesis, particularly Photosystem II (PSII), is significantly affected by heat stress [ 9 , 10 ], with moderate heat causing PSII photoinhibition [ 11 ], while higher temperatures can lead to dissociation or inhibition of the oxygen-evolving complex [ 12 ]. Although Rubisco, the enzyme responsible for carbon fixation, is intrinsically thermostable in higher plants, heat stress can inhibit Rubisco activase, thereby impacting carbon assimilation rates [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Elevated Temperature Effects on Protein Turnover Dynamics in Arabidopsis thaliana Seedlings Revealed by 15N-Stable Isotope Labeling and ProteinTurnover Algorithm

Fan,

Xu,

Hegeman

2024

IJMS

View full text Add to dashboard Cite

Global warming poses a threat to plant survival, impacting growth and agricultural yield. Protein turnover, a critical regulatory mechanism balancing protein synthesis and degradation, is crucial for the cellular response to environmental changes. We investigated the effects of elevated temperature on proteome dynamics in Arabidopsis thaliana seedlings using 15N-stable isotope labeling and ultra-performance liquid chromatography-high resolution mass spectrometry, coupled with the ProteinTurnover algorithm. Analyzing different cellular fractions from plants grown under 22 °C and 30 °C growth conditions, we found significant changes in the turnover rates of 571 proteins, with a median 1.4-fold increase, indicating accelerated protein dynamics under thermal stress. Notably, soluble root fraction proteins exhibited smaller turnover changes, suggesting tissue-specific adaptations. Significant turnover alterations occurred with redox signaling, stress response, protein folding, secondary metabolism, and photorespiration, indicating complex responses enhancing plant thermal resilience. Conversely, proteins involved in carbohydrate metabolism and mitochondrial ATP synthesis showed minimal changes, highlighting their stability. This analysis highlights the intricate balance between proteome stability and adaptability, advancing our understanding of plant responses to heat stress and supporting the development of improved thermotolerant crops.

show abstract

Nanoscale simulation of the thylakoid membrane response to extreme temperatures

Cited by 5 publications

References 106 publications

Hydrophobic Mismatch in the Thylakoid Membrane Regulates Photosynthetic Light Harvesting

Hydrophobic Mismatch in the Thylakoid Membrane Regulates Photosynthetic Light Harvesting

Influence of Elevated CO2 and Temperature on Yield Attributes of Rice and Wheat in Central India

Elevated Temperature Effects on Protein Turnover Dynamics in Arabidopsis thaliana Seedlings Revealed by 15N-Stable Isotope Labeling and ProteinTurnover Algorithm

Contact Info

Product

Resources

About