Nanoscale surface topography enhances cell adhesion and gene expression of madine darby canine kidney cells

Cheng, Jinghui; Zhu, Bangshang; Wang, X. F.; Lu, Qinghua; Chen, W. T.; Zhou, Xin

doi:10.1007/s10856-007-3323-z

Cited by 25 publications

(17 citation statements)

References 37 publications

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“…Key to this is that nanogrooved surfaces may induce enhanced tissue organization and facilitate active self-assembly of ECM molecules to further mediate cell attachment and orientation. Indeed, the elongated morphology and alignment induced by grooved substrates may resemble the natural state of many cell populations in vivo and is observed to occur in a wide range of cell types, including fibroblasts,91 osteoblasts,92 nerve cells,93 and MSCs,88 which respond profoundly to grooved substrates and have been shown to upregulate the expression of components of the ECM94 as well as proteins central to cellular adhesion95 and the transduction of mechanical forces 96…”

Section: Effects Of Nanoscale Grooves On Cell Adhesionmentioning

confidence: 99%

Nanotopographical modification: a regulator of cellular function through focal adhesions

Biggs

Richards

Dalby

2010

Nanomedicine: Nanotechnology, Biology and Medicine

427

327

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As materials technology and the field of biomedical engineering advances, the role of cellular mechanisms, in particular adhesive interactions with implantable devices, becomes more relevant in both research and clinical practice. A key tenet of medical device design has evolved from the exquisite ability of biological systems to respond to topographical features or chemical stimuli, a process that has led to the development of next-generation biomaterials for a wide variety of clinical disorders. In vitro studies have identified nanoscale features as potent modulators of cellular behavior through the onset of focal adhesion formation. The focus of this review is on the recent developments concerning the role of nanoscale structures on integrin-mediated adhesion and cellular function with an emphasis on the generation of medical constructs with regenerative applications. KeywordsFocal adhesions; Biomaterials; Nanotopography; Cell signaling This review highlights the importance and development of the physiomechanical processes that regulate early cell-biomaterial interactions and the influence of nanoscale topographical modification on integrin-mediated cellular adhesion. As materials technology and the field of tissue engineering advance, the role of cellular adhesive mechanisms, in particular the interactions with implantable materials, becomes more relevant in both research and clinical practice.Biomaterials are never truly inert, being at best biotolerable. The cell-substratum interface functions as more than a simple boundary of definition between the host and an implanted device; instead, it presents primary cues for cellular adhesion and the subsequent induction of tissue integration. Indeed, the cytocompatibility of a material can be assessed in vitro by observing the viability and biofunctionality of cells at the substratum interface, paving the way for in vivo studies into device functionality. The range of materials currently designated as biomedically useful and their lack of biofunctionality reflects an increasing need for biomimetic constructs but also indicates the challenges present within the field. In particular a need exists to create truly biocompatible devices and ultimately to control the interactions that occur at the cell-substratum interface.A key tenet of medical device design has evolved from the exquisite ability of biological systems to respond to topographical features or chemical stimuli, a process that has led to the NIH Public Access Author ManuscriptNanomedicine. Author manuscript; available in PMC 2010 October 28. Published in final edited form as:Nanomedicine. 2010 October ; 6(5): 619-633. doi:10.1016/j.nano.2010.01.009. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript development of next-generation biomaterials. Recently published in the journal Science are the prerequisites for third generation biomaterials; not only should they support the healing site (as first-generation biomaterials), but they should be bioactive and possibly biodegradable...

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Section: Effects Of Nanoscale Grooves On Cell Adhesionmentioning

confidence: 99%

Nanotopographical modification: a regulator of cellular function through focal adhesions

Biggs

Richards

Dalby

2010

Nanomedicine: Nanotechnology, Biology and Medicine

427

327

View full text Add to dashboard Cite

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“…Topographic organization of cells has been used to modulate the phenotype of osteoblasts [85], cardiomyocytes [86], and fibroblasts [87]. Nanogrooves specifically have been used to organize epithelial cells in the direction of the nanogrooves: MDCK [88, 89], human corneal epithelial cells [90] (Figure 2(c)), and in human mesenchymal stem cells [66]. Nanogroove topography could be used in a TE system to organize airway epithelium along nanogrooves.…”

Section: Engineering Approaches To Control Epithelial Regenerationmentioning

confidence: 99%

Engineering Airway Epithelium

Soleas

Paz

Marcus

et al. 2012

Journal of Biomedicine and Biotechnology

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Airway epithelium is constantly presented with injurious signals, yet under healthy circumstances, the epithelium maintains its innate immune barrier and mucociliary elevator function. This suggests that airway epithelium has regenerative potential (I. R. Telford and C. F. Bridgman, 1990). In practice, however, airway regeneration is problematic because of slow turnover and dedifferentiation of epithelium thereby hindering regeneration and increasing time necessary for full maturation and function. Based on the anatomy and biology of the airway epithelium, a variety of tissue engineering tools available could be utilized to overcome the barriers currently seen in airway epithelial generation. This paper describes the structure, function, and repair mechanisms in native epithelium and highlights specific and manipulatable tissue engineering signals that could be of great use in the creation of artificial airway epithelium.

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“…K22E [20]. Overexpression of K22E can enhance the cell adhesion and cell growth of MDCK [21]. TRXR1 can inhibit the production of ROS and play an important role in the oxido-reduction signaling pathway [22].…”

Section: Functional Grouping Of Differentially Expressed Proteinsmentioning

confidence: 99%

Differentially Expressed Protein Profile of Renal Tubule Cell Stimulated by Elevated Uric Acid Using SILAC Coupled to LC-MS

Hong

Xue

Liu

et al. 2011

Cell Physiol Biochem

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Background/Aims: Hyperuricemia could lead to serious renal disease. It will advance our understanding of the mechanism of this disease to study the differentially expressed protein in renal tubular epithelial cells stimulated by elevated uric acid. Methods: We used SILAC coupled to LC-MS to study differentially expressed protein profile and to analysis the functional status of renal tubular epithelial cells stimulated by 600µM uric acid, and to investigate the potential biology function. The MS results were analyzed by online platform and further confirmed by western blotting. Results: 789 differentially expressed proteins were identified, of which 42 proteins and 49 proteins were related with cell proliferation and cell apoptosis, respectively. Pathway analysis showed that MAPK signaling pathway was a key pathway related to the function of these proteins. In addition, prohibitin-2 was identified to be related to renal cell transdifferentiation stimulated by elevated uric acid. Conclusion: This work provides an overview of protein

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Nanoscale surface topography enhances cell adhesion and gene expression of madine darby canine kidney cells

Cited by 25 publications

References 37 publications

Nanotopographical modification: a regulator of cellular function through focal adhesions

Nanotopographical modification: a regulator of cellular function through focal adhesions

Engineering Airway Epithelium

Differentially Expressed Protein Profile of Renal Tubule Cell Stimulated by Elevated Uric Acid Using SILAC Coupled to LC-MS

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