Nanoscale x-ray holo-tomography of human brain tissue with phase retrieval based on multiphoton energy recordings

Robisch, Anna-Lena; Eckermann, Marina; Töpperwien, Mareike; Meer, Franziska van der; Stadelmann, Christine; Salditt, Tim

doi:10.1117/12.2529041

Developments in X-Ray Tomography XII 2019

DOI: 10.1117/12.2529041

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Nanoscale x-ray holo-tomography of human brain tissue with phase retrieval based on multiphoton energy recordings

Anna-Lena Robisch

Marina Eckermann

Mareike Töpperwien

et al.

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2020

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Cited by 2 publications

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“…It is published in the corresponding conference proceedings. 38 Apart from that, no conflicts of interest, financial or otherwise, are declared by the authors.…”

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confidence: 98%

Nanoscale x-ray holotomography of human brain tissue with phase retrieval based on multienergy recordings

et al. 2020

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X-ray cone-beam holotomography of unstained tissue from the human central nervous system reveals details down to subcellular length scales. This visualization of variations in the electron density of the sample is based on phase-contrast techniques using intensities formed by self-interference of the beam between object and detector. Phase retrieval inverts diffraction and overcomes the phase problem by constraints such as several measurements at different Fresnel numbers for a single projection. Therefore, the object-to-detector distance (defocus) can be varied. However, for cone-beam geometry, changing defocus changes magnification, which can be problematic in view of image processing and resolution. Alternatively, the photon energy can be altered (multi-E). Far from absorption edges, multi-E data yield the wavelength-independent electron density. We present the multi-E holotomography at the Göttingen Instrument for Nano-Imaging with X-Rays (GINIX) setup of the P10 beamline at Deutsches Elektronen-Synchrotron. The instrument is based on a combined optics of elliptical mirrors and an x-ray waveguide positioned in the focal plane for further coherence, spatial filtering, and high numerical aperture. Previous results showed the suitability of this instrument for nanoscale tomography of unstained brain tissue. We demonstrate that upon energy variation, the focal spot is stable enough for imaging. To this end, a doublecrystal monochromator and automated alignment routines are required. Three tomograms of human brain tissue were recorded and jointly analyzed using phase retrieval based on the contrast transfer function formalism generalized to multiple photon energies. Variations of the electron density of the sample are successfully reconstructed. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…It is published in the corresponding conference proceedings. 38 Apart from that, no conflicts of interest, financial or otherwise, are declared by the authors.…”

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confidence: 98%

Nanoscale x-ray holotomography of human brain tissue with phase retrieval based on multienergy recordings

et al. 2020

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Compression of 3D dynamic holographic scenes in the Fresnel domain

Trejos¹,

Gómez²,

Velez-Zea³

et al. 2020

Appl. Opt.

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In this paper we present an optodigital protocol for the compression of 3D dynamic scenes recorded with an off-axis Fresnel holographic system. The compression protocol involves optical scaling, sampling with binary masks, and multiplexing of the optical field data obtained after a filtering process applied to Fresnel holograms. Volume reduction of up to 93.71% and a 16-fold decrease in the transfer time are achieved. Virtual-optical reconstruction is performed for different values of the parameters involved in the compression protocol. The correlation coefficient is used as a metric to measure the loss caused by the volume reduction process. Furthermore, we show that a high level of lossy compression can be achieved with this protocol, with better reconstruction quality than the MPEG-4 video compression technique. Finally, we perform the experimental reconstruction using a holographic projection system based on a phase-only spatial light modulator, thus highlighting the potential of our proposal.

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Nanoscale x-ray holo-tomography of human brain tissue with phase retrieval based on multiphoton energy recordings

Cited by 2 publications

References 28 publications

Nanoscale x-ray holotomography of human brain tissue with phase retrieval based on multienergy recordings

Nanoscale x-ray holotomography of human brain tissue with phase retrieval based on multienergy recordings

Compression of 3D dynamic holographic scenes in the Fresnel domain

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