Nanoscaled Biocoatings via Enzyme Mediated Autodeposition of Casein

Ruediger, Arne A.; Bremser, Wolfgang; Strube, Oliver I.

doi:10.1002/mame.201600034

Cited by 12 publications

(21 citation statements)

References 68 publications

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“…On top and around the high enzyme accumulation, lots of particles are found, whereas the density of deposition is reduced proportionally to the distance from the aggregate. This previously seen effect [13] is also valid for the EMA with silver particles, which again proves the indifference of the result from specific variations.…”

Section: Enzyme-medatied Addressingsupporting

confidence: 72%

“…The underlying principle is directly connected to the special structure of a casein micelle, which is comprised of a hydrophobic core, stabilized by hydrophilic parts of the amphiphilic κ-casein. Although the actual composition of a casein micelle is much more complex, this simplified model is sufficient to understand its colloidal properties and stabilization mechanisms [13,14].…”

Section: Biomimetic Hybrid Particlesmentioning

confidence: 99%

“…On the other hand, to yield evenly distributed surface enzyme concentration, the enzyme was also covalently bound to the glass surface, via epoxy functionalized supports [13]. The molecular process of this coupling is well understood [31].…”

Section: Enzyme-medatied Addressingmentioning

confidence: 99%

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Site-Specific Addressing of Particles and Coatings via Enzyme-Mediated Destabilization

Wedegärtner

Strube

2019

Catalysts

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Enzyme mediated addressing (EMA) is a highly specific and easy-to-apply technology for direction and deposition of particles and coatings on surfaces. Key feature of this process is an enzymatic reaction in direct proximity to the surface, which induces the deposition. The technique has previously shown great success in the handling of biological particles. In this study, addressing of non-biological nanoparticles, in particular plastics and metals, is presented. The respective particles are stabilized by an amphiphilic, enzyme-degradable block copolymer, consisting of poly(ethylene glycol) and poly(caprolactone). After contact with the enzyme pseudomonas lipase, the particles are destabilized, due to the loss of the hydrophilic part of the block copolymer. The lipase is therefore immobilized on glass supports. Immobilization is performed via adsorption or covalent bonding to epoxide groups. All deposition experiments show that addressing of individual particles occurs precisely within the predefined areas of enzyme activity. Depending on the material and reaction conditions, intact nanoparticles or coatings from such can be gained. The quintessence of the study is the indifference of the EMA regarding particle materials. From this rationale, the technique offers near unlimited materials compatibility within a precise, easy-to-apply, and upscalable process.

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Section: Enzyme-medatied Addressingsupporting

confidence: 72%

Section: Biomimetic Hybrid Particlesmentioning

confidence: 99%

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Site-Specific Addressing of Particles and Coatings via Enzyme-Mediated Destabilization

Wedegärtner

Strube

2019

Catalysts

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“…This confirms the model of the enzyme-mediated autodeposition and verifies its high site-specificity. 105 Figure 6 shows SEM images of the results.…”

Section: Casein Deposition Via Covalently Immobilized Enzyme Aggregatesmentioning

confidence: 98%

“…105 To this, chymosin can be covalently immobilized at low ionic strength via diepoxy-PEG-spacers with a molecular weight of 20.000 g/mol (persistence length is about 160 nm) on aminated supports. PEG is chosen, because it exhibits good water solubility and is known to preserve enzymatic activity after covalent attachment by providing high mobility and flexibility.…”

Section: Casein Deposition Via Covalently Immobilized Enzyme Aggregatesmentioning

confidence: 99%

The enzyme-mediated autodeposition of casein: effect of enzyme immobilization on deposition of protein structures

2016

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The enzyme-mediated autodeposition on the example of casein is reviewed, and deposition of casein structures is investigated in relation to applied immobilization methods of enzyme. First, casein is described in detail with respect to its structure in aqueous environments, followed by presentation of historical and current nonfood applications of casein. The presented process uses enzymes to trigger deposition of bio-based particles in close proximity to a support surface and allows for a high control over film formation and site-specificity. This is ensured by immobilization of enzyme on the support material. The herein described system is based on casein as protein and chymosin as enzyme. Different immobilization methods are investigated with respect to obtainable casein coatings, layers, and structures. Physical adsorption of enzyme enables the formation of casein coatings with controllable film thickness and is suitable for in situ buildup of adhesive layers due to diffusion of enzyme. The highest control over film formation is provided by covalent attachment of enzyme. Based on the attained results, the enzymemediated autodeposition gives new insights into biological material design. Graphical abstractEnzyme Casein micelle intact Bulk zone (no enzyme) Reaction zone Phe Met Support Support Casein micelle cleaved

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Enzyme‐Mediated In Situ Buildup and Site‐Specific Addressing of Polymeric Coatings

Appel

Wedegärtner

Lüther

et al. 2018

Macro Materials & Eng

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Formation of site‐specific deposition of polymeric particles and films with controllable parameters via enzyme‐mediated addressing is investigated. The polymers are synthesized in situ by combining the addressing process with enzyme‐mediated radical polymerization, using the enzyme horseradish peroxidase. As a model system, the technically important poly(methyl methacrylate) is formed and addressed on both glass‐ and polyethylene supports.The key to the controllability is the influence of different reaction parameters on particle formation, namely, the concentration of H2O2 and the pH show the most significant impact. With higher concentrations of H2O2, size and amount of gained particles increases, due to H2O2 being a limiting factor to the reaction. Moreover, since acetyl acetone forms the end‐group of the polymer chains, the pH directly influences the particle stability in addition to affecting the enzyme activity. This effect can be utilized to gain either films or particle assemblies at will. Deposition occurs highly site‐specific, exclusively on enzyme‐functionalized areas, enabling patterned coating structures within a safe, energy efficient, and easy‐to‐apply process.

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Nanoscaled Biocoatings via Enzyme Mediated Autodeposition of Casein

Cited by 12 publications

References 68 publications

Site-Specific Addressing of Particles and Coatings via Enzyme-Mediated Destabilization

Site-Specific Addressing of Particles and Coatings via Enzyme-Mediated Destabilization

The enzyme-mediated autodeposition of casein: effect of enzyme immobilization on deposition of protein structures

Enzyme‐Mediated In Situ Buildup and Site‐Specific Addressing of Polymeric Coatings

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