2020
DOI: 10.1038/s41598-020-60977-5
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Nanoscopic X-ray tomography for correlative microscopy of a small meiofaunal sea-cucumber

Abstract: In the field of correlative microscopy, light and electron microscopy form a powerful combination for morphological analyses in zoology. Due to sample thickness limitations, these imaging techniques often require sectioning to investigate small animals and thereby suffer from various artefacts. A recently introduced nanoscopic X-ray computed tomography (NanoCT) setup has been used to image several biological objects, none that were, however, embedded into resin, which is prerequisite for a multitude of correla… Show more

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Cited by 13 publications
(13 citation statements)
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“…The cell cytoplasm appears as a white ring. Note that samples for HXT were stained with osmium tetroxide to increase contrast on cell membranes ( 76, 77 ). The cell cytoplasm was segmented on each slice to produce the 3D rendering of the entire cell presented in Movie S1.…”
Section: Supplementary Materials Formentioning
confidence: 99%
“…The cell cytoplasm appears as a white ring. Note that samples for HXT were stained with osmium tetroxide to increase contrast on cell membranes ( 76, 77 ). The cell cytoplasm was segmented on each slice to produce the 3D rendering of the entire cell presented in Movie S1.…”
Section: Supplementary Materials Formentioning
confidence: 99%
“…12, 13). In the field of anatomy, µCT has been demonstrated to be a useful analytical tool applied in a rapidly growing variety of metazoan taxa, such as cnidarians [100], plathelminths [101], nematomorphs [99], nematodes [102], polychaetes [103,104], molluscs [98,105], echinoderms [106,107] as well as arthropods [108][109][110][111][112][113]. Modern, lab-based µCT-scanners deliver high resolution allowing the investigation of tiny specimens with body sizes of free-living crustacean larvae ranging from 75-195 µm in Tantulocarida [114], the smallest arthropods in the world, up to 5 cm in length, e.g., in Stomatopoda [115].…”
Section: Additional Commentsmentioning
confidence: 99%
“…morphology, chemistry, structure) are linked. This approach is currently a developing application in human-made materials research [30][31][32][33] and life/biological science [34][35][36][37] , and despite a few geological [38][39][40] and more recently specific soil science examples 41,42 , the method has not been applied to soil weathering.…”
Section: Introductionmentioning
confidence: 99%