Nanosensors for next generation drug screening

Kannam, Sridhar Kumar; Downton, Matthew T.; Gunn, Natalie; Kim, Sung‐Cheol; Rogers, Priscilla; Schieber, Christine; Baldauf, Julia; Wagner, John; Scott, Daniel J.; Bathgate, Ross A. D.; Skafidas, Efstratios; Harrer, Stefan

doi:10.1117/12.2033737

Cited by 3 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resistive pulse sensing can be used to investigate a broad variety of molecular biological systems with single-particle, single nucleotide and even single-binding site resolution. It is explicitly powerful to perform next-generation nanopore-based DNA-sequencing [10][11][12][13][14][15][16] and to investigate target-ligand interactions in the pre-clinical compound-to-hit, hit-to-lead, and lead optimization stages of the pharma pipeline [17][18][19][20][21][22] . Figure 1.…”

Section: Novel Sensors For Precision Medicine Applicationsmentioning

confidence: 99%

Front Matter: Volume 9517

Sánchez‐Rojas¹,

Brama²

2015

SPIE Proceedings

View full text Add to dashboard Cite

The papers included in this volume were part of the technical conference cited on the cover and title page. Papers were selected and subject to review by the editors and conference program committee. Some conference presentations may not be available for publication. The papers published in these proceedings reflect the work and thoughts of the authors and are published herein as submitted. The publisher is not responsible for the validity of the information or for any outcomes resulting from reliance thereon.Please use the following format to cite material from this book:Publication of record for individual papers is online in the SPIE Digital Library. SPIEDigitalLibrary.orgPaper Numbering: Proceedings of SPIE follow an e-First publication model, with papers published first online and then in print. Papers are published as they are submitted and meet publication criteria. A unique citation identifier (CID) number is assigned to each article at the time of the first publication. Utilization of CIDs allows articles to be fully citable as soon as they are published online, and connects the same identifier to all online, print, and electronic versions of the publication. SPIE uses a six-digit CID article numbering system in which:The first four digits correspond to the SPIE volume number. The last two digits indicate publication order within the volume using a Base 36 numbering system employing both numerals and letters. These two-number sets start with 00, 01, 02, 03, 04, 05, 06, 07, 08, 09, 0A, 0B … 0Z, followed by 10-1Z, 20-2Z, etc.The CID Number appears on each page of the manuscript. The complete citation is used on the first page, and an abbreviated version on subsequent pages. Proc. of SPIE Vol. 9517 951701-2 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 07/14/2015 Terms of Use: http://spiedl.org/terms

show abstract

Section: Novel Sensors For Precision Medicine Applicationsmentioning

confidence: 99%

Front Matter: Volume 9517

Sánchez‐Rojas¹,

Brama²

2015

SPIE Proceedings

View full text Add to dashboard Cite

show abstract

“…Based on this framework we have proposed a resistive pulse sensing approach to allow ultra-high-throughput screening of drug target/test drug binding and activation by compounds with single-particle screening sensitivity [115][116][117]. Due to their dominant role in the biomatrix, their growing importance in the context of the pharma market, and the existence of functionality-preserving extraction methods from the cell environment, we have identified G-Protein Coupled Receptors (GPCRs) as a drug target class of special interest.…”

Section: Resistive Pulse Sensing Of Target-ligand Interactionsmentioning

confidence: 99%

Label-free screening of single biomolecules through resistive pulse sensing technology for precision medicine applications

et al. 2015

View full text Add to dashboard Cite

Employing integrated nano- and microfluidic circuits for detecting and characterizing biological compounds through resistive pulse sensing technology is a vibrant area of research at the interface of biotechnology and nanotechnology. Resistive pulse sensing platforms can be customized to study virtually any particle of choice which can be threaded through a fluidic channel and enable label-free single-particle interrogation with the primary read-out signal being an electric current fingerprint. The ability to perform label-free molecular screening with single-molecule and even single binding site resolution makes resistive pulse sensing technology a powerful tool for analyzing the smallest units of biological systems and how they interact with each other on a molecular level. This task is at the core of experimental systems biology and in particular 'omics research which in combination with next-generation DNA-sequencing and next-generation drug discovery and design forms the foundation of a novel disruptive medical paradigm commonly referred to as personalized medicine or precision medicine. DNA-sequencing has approached the 1000-Dollar-Genome milestone allowing for decoding a complete human genome with unmatched speed and at low cost. Increased sequencing efficiency yields massive amounts of genomic data. Analyzing this data in combination with medical and biometric health data eventually enables understanding the pathways from individual genes to physiological functions. Access to this information triggers fundamental questions for doctors and patients alike: what are the chances of an outbreak for a specific disease? Can individual risks be managed and if so how? Which drugs are available and how should they be applied? Could a new drug be tailored to an individual's genetic predisposition fast and in an affordable way? In order to provide answers and real-life value to patients, the rapid evolvement of novel computing approaches for analyzing big data in systems genomics has to be accompanied by an equally strong effort to develop next-generation DNA-sequencing and next-generation drug screening and design platforms. In that context lab-on-a-chip devices utilizing nanopore- and nanochannel based resistive pulse-sensing technology for DNA-sequencing and protein screening applications occupy a key role. This paper describes the status quo of resistive pulse sensing technology for these two application areas with a special focus on current technology trends and challenges ahead.

show abstract

Translational diffusion of proteins in nanochannels

Kannam

Downton

2017

The Journal of Chemical Physics

View full text Add to dashboard Cite

Hydrodynamic interactions play an important role in the transport of analytes through nanoscale devices. Of particular note is the role that no-slip boundary conditions have on the drag coefficient of confined particles and molecules. In this work, we use a coarse grained molecular dynamics model to measure the diffusion coefficients of proteins confined within cylindrical nanochannels of similar dimension. Finite-size corrected bulk diffusion coefficients are found to agree with experimental data, while in channels, a good match is found between theoretical expressions based on continuum fluid mechanics and the reduction of the translational diffusion coefficient across a range of protein to channel size ratios. These results demonstrate that it is possible to directly use molecular simulation to make quantitative predictions of the effects of hydrodynamics on diffusion at length scales of order 1 nm.

show abstract

Nanosensors for next generation drug screening

Cited by 3 publications

References 13 publications

Front Matter: Volume 9517

Front Matter: Volume 9517

Label-free screening of single biomolecules through resistive pulse sensing technology for precision medicine applications

Translational diffusion of proteins in nanochannels

Contact Info

Product

Resources

About