Nanoshells for Surface-Enhanced Raman Spectroscopy in Eukaryotic Cells: Cellular Response and Sensor Development

Ochsenkühn, Michael A.; Jess, P.; Stoquert, Helene C; Dholakia, Kishan; Campbell, Colin J.

doi:10.1021/nn900681c

Cited by 96 publications

(109 citation statements)

References 48 publications

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“…In addition, the pgSERS spectra contained many features assignable to components at the cell membrane, such as saccharides (1,490, 1,268, 850, and 647 cm Ϫ1 ), lipids (1,452, 1,430, 1,378, 1,300, 1,079, 1,049, and 717 cm Ϫ1 ), and proteins (1,612, 1,365, 1,269, 1,216, 1,179, 1,015, 850, and 762 cm Ϫ1 ). Interestingly, the strong peaks at 1,216 and 1,269 cm Ϫ1 are characteristic of ␤-sheet and ␣-helix protein secondary structures, respectively (10,11). This is consistent with the presence of the ␤-barrel proteins (porins) across the outer membrane of Gram-negative bacteria (15) and an abundance of ␣-helical transmembrane proteins.…”

supporting

confidence: 74%

Peptide-Guided Surface-Enhanced Raman Scattering Probes for Localized Cell Composition Analysis

Athamneh

Senger

2012

Appl Environ Microbiol

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The ability to control the localization of surface-enhanced Raman scattering (SERS) nanoparticle probes in bacterial cells is critical to the development of analytical techniques that can nondestructively determine cell composition and phenotype. Here, selective localization of SERS probes was achieved at the outer bacterial membrane by using silver nanoparticles functionalized with synthetic hydrophobic peptides.T he ability to chemically characterize microbial phenotypes in real time and nondestructively is a critical development for both industrial and clinical microbiology. Surface-enhanced Raman scattering (SERS) methods are of interest for this task because of their exceptional analytical sensitivity with nanometer scale localized selectivity (1, 2, 21). With SERS, Raman scattering from a molecule is enhanced several orders of magnitude when it is in the proximity of a metal substrate (1). This has profound potential for the investigation of biological systems (21). However, the extreme biomolecular complexity of the intracellular matrix poses a major challenge to the use of SERS to study microbial phenotypes and cell composition (2). SERS spectra of bacteria are highly irreproducible and difficult to interpret because metal nanoparticles, often used as SERS substrates (or probes), disperse randomly throughout the cell (2,12,16,21). This approach results in convoluted SERS spectra composed of contributions from biochemicals of diverse intracellular environments simultaneously. Therefore, the ability to control the localization of SERS probes inside the cell is critical to the production of minimal SERS spectra capable of being deconvoluted to resolve chemical composition and phenotype data for localized intracellular environments independently.Metal nanoparticles, conjugated with a variety of ligands, have been used as intracellular SERS probes with exceptional selectivity for the purposes of imaging and detection, but these have not been designed to determine cell composition or phenotype (4,14,17,20). Zeiri et al. (24) developed protocols to direct the production of silver nanoparticles (SNPs) to either the cytosol of a bacterium or to the cell wall. The resulting SERS spectra reflected the biochemical environment surrounding SNPs. However, intracellular synthesis of SNPs required the use of toxic reagents and the potential to localize these SNPs was limited (2). Recently, Xie et al. (22) developed nuclear targeting SERS probes by using gold nanoparticles functionalized with a nuclear localization signal peptide. The probes were used to generate SERS spectra of the HeLa cell nucleus, and these spectra were found to contain abundant information about the nuclear environment. Despite their usefulness, these probes are limited to eukaryotic cells and target only a single intracellular environment. Since the nucleus is relatively large and chemically diverse, the resulting spectra contained substantial variation (22).Here, we demonstrate the ability to direct SERS probes to the outer membrane of Escherichia...

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supporting

confidence: 74%

Peptide-Guided Surface-Enhanced Raman Scattering Probes for Localized Cell Composition Analysis

Athamneh

Senger

2012

Appl Environ Microbiol

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“…Quantitative assignment of peaks is always challenging in SERS analysis of an inhomogeneous sample, but a number of peaks appear at locations consistent with biological molecules. Peaks at 830, 1,295, 1,316, 1,438 and 1,444 cm À 1 are consistent with lipid components 20,21 , while 932, 968, 1,073, 1,159, 1,270 cm À 1 are commonly seen in protein (amide) or amino-acid bands 22,23 . Nucleic acids such as adenosine and guanosine are also readily detected by SERS and although often observed closer to 730 cm À 1 , the ring-breathing mode of adenosine is reported to undergo a shift, depending on the orientation to the SERS surface, and may be responsible for the strong peak around 749 cm À 1 (refs 24,25)…”

Section: Resultsmentioning

confidence: 68%

Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

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Nanoparticle manipulation is of increasing interest, since they can report single moleculelevel measurements of the cellular environment. Until now, however, intracellular nanoparticle locations have been essentially uncontrollable. Here we show that by infusing a gold ion solution, focused laser light-induced photoreduction allows in situ fabrication of gold nanoparticles at precise locations. The resulting particles are pure gold nanocrystals, distributed throughout the laser focus at sizes ranging from 2 to 20 nm, and remain in place even after removing the gold solution. We demonstrate the spatial control by scanning a laser beam to write characters in gold inside a cell. Plasmonically enhanced molecular signals could be detected from nanoparticles, allowing their use as nano-chemical probes at targeted locations inside the cell, with intracellular molecular feedback. Such light-based control of the intracellular particle generation reaction also offers avenues for in situ plasmonic device creation in organic targets, and may eventually link optical and electron microscopy.

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“…Nanosensors to measure intracellular pH have been reported in many studies, the most common being 4-mercaptobenzoic acid functionalised nanoparticles. [84][85][86][87] Auchinvole et al [88] also developed nanosensors for measurement of intracellular redox potential, a key physiological characteristic, with changes in redox potential often associated with disease development. This is therefore a key characteristic that could be monitored in HTS assays to monitor response to drug treatment.…”

Section: An Alternative Technique -Surface Enhanced Raman Spectroscopmentioning

confidence: 99%

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Jamieson

Byrne²

2017

Vibrational Spectroscopy

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. (2017). Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? Vibrational Spectroscopy, vol. 91, July, pp. 16-30. doi.org/10.1016/ j.vibspec.2016 Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? AbstractVibrational spectroscopy is currently widely explored as a tool in biomedical applications. An area at the forefront of this field is the use of vibrational spectroscopy for disease diagnosis, ultimately aiming towards spectral pathology. However, while this field shows promising results, moving this technique into the clinic faces the challenges of widespread clinical trials and legislative approval. While spectral pathology has received a lot of attention, there are many other biomedical applications of vibrational spectroscopy, which could potentially be translated to applications with greater ease. A particularly promising application is the use of vibrational spectroscopic techniques to study the interaction of drugs with cells. Many studies have demonstrated the ability to detect biochemical changes in cells in response to drug application, using both infrared and Raman spectroscopy. This has shown potential for use in high throughput screening (HTS) applications, for screening of efficacy and mode of action of potential drug candidates, to speed up the drug discovery process. HTS is still a relatively new and growing area of research and, therefore, there is more potential for new techniques to move into and shape this field. Vibrational spectroscopic techniques come with many benefits over the techniques used currently in HTS, primarily based on fluorescence assays to detect specific binding interactions or phenotypes. They are label free, and an infrared or Raman spectrum provides a wealth of biochemical information, and therefore could reveal not only information about a specific interaction, but about how the overall biochemistry of a cell changes in response to application of a drug candidate. Therefore, drug mode of action could be elucidated. This review will investigate the potential for vibrational spectroscopy, particularly FTIR and Raman spectroscopy, to benefit the field of HTS and improve the drug development process. In addition to FTIR and Raman spectroscopy, surface enhanced Raman spectroscopy (SERS), coherent anti-Stokes Raman spectroscopy (CARS) and stimulated Raman spectroscopy (SRS), will be investigated as an alternative tool in the HTS process.

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Nanoshells for Surface-Enhanced Raman Spectroscopy in Eukaryotic Cells: Cellular Response and Sensor Development

Cited by 96 publications

References 48 publications

Peptide-Guided Surface-Enhanced Raman Scattering Probes for Localized Cell Composition Analysis

Peptide-Guided Surface-Enhanced Raman Scattering Probes for Localized Cell Composition Analysis

Laser-targeted photofabrication of gold nanoparticles inside cells

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

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