Nanostars on Nanopipette Tips: A Raman Probe for Quantifying Oxygen Levels in Hypoxic Single Cells and Tumours

Nguyen, Thanh-Danh; Song, Min Seok; Ly, Nguyễn Hoàng; Lee, So Yeong; Joo, Sang‐Woo

doi:10.1002/anie.201812677

Cited by 61 publications

(50 citation statements)

References 54 publications

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“…Among these, O 2 plays a critical role in the cell growth and proliferation, and thus its high concentrations have been correlated with both increased cellularity [3] and cell viability [4]. Conversely, a deficiency in O 2 can result in a hypoxic cell state, which is commonly associated with decreased metabolic activity and potentially undesirable differentiation behavior [5][6][7]. Hence, optimal oxygen transport is important in maintaining tissue function and overall survival within the artificial tissues.…”

Section: Introductionmentioning

confidence: 99%

Scaffolds with a High Surface Area-to-Volume Ratio and Cultured Under Fast Flow Perfusion Result in Optimal O2 Delivery to the Cells in Artificial Bone Tissues

et al. 2019

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Tissue engineering has the potential for repairing large bone defects, which impose a heavy financial burden on the public health. However, difficulties with O2 delivery to the cells residing in the interior of tissue engineering scaffolds make it challenging to grow artificial tissues of clinically-relevant sizes. This study uses image-based simulation in order to provide insight into how to better optimize the scaffold manufacturing parameters, and the culturing conditions, in order to resolve the O2 bottleneck. To do this, high resolution 3D X-ray images of two common scaffold types (salt leached foam and non-woven fiber mesh) are fed into Lattice Boltzmann Method fluid dynamics and reactive Lagrangian Scalar Tracking mass transfer solvers. The obtained findings indicate that the scaffolds should have maximal surface area-to-solid volume ratios for higher chances of the molecular collisions with the cells. Furthermore, the cell culture media should be flown through the scaffold pores as fast as practically possible (without detaching or killing the cells). Finally, we have provided a parametric sweep that maps how the molecular transport within the scaffolds is affected by variations in rates of O2 consumption by the cells. Ultimately, the results of this study are expected to benefit the computer-assisted design of tissue engineering scaffolds and culturing experiments.

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Section: Introductionmentioning

confidence: 99%

Scaffolds with a High Surface Area-to-Volume Ratio and Cultured Under Fast Flow Perfusion Result in Optimal O2 Delivery to the Cells in Artificial Bone Tissues

et al. 2019

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“…Theoretical calculations were performed using our previous report using Gaussian program 36 . Both the Raman and infrared frequencies and their plausible assignments were obtained at the fully optimized geometry with the estimated minima on the potential energy surfaces.…”

Section: Methodsmentioning

confidence: 99%

Vibrational Spectroscopic Estimation of Semivolatile Organic Compound Evaporation From Glass Surfaces

Lee

Jang

et al. 2020

Bulletin Korean Chem Soc

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The evaporation of the semivolatile organic compound (SVOC) benzothiazole (BZT) from glass surfaces has been estimated by means of surface-enhanced Raman scattering (SERS) and gas-phase Fouriertransform infrared (FTIR) spectroscopy. The FTIR spectrum in the gas cell indicates the presence of BZT in the air. The density functional theory (DFT) of the vibrational spectra of BZT was tested using the B3LYP/6-311G++(d,p) basis sets with potential energy distribution (PED) analysis. The BZT thin film could be investigated using SERS by dropping a gold nanoparticle (AuNP) solution on glass surfaces. The intensified modes in the SERS spectra suggest the plausible binding modes of BZT via its N 11 atom on AuNPs. A decrease in the SERS spectra indicate a substantial evaporation of BZT. The concentration of BZT in the air could be estimated as low as~0.8% according to the infrared spectra after 24 h.

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“…Subcellular microenvironments such as pH, the content of ROS, biomolecules, and redox potential are important parameters for ensuring normal cell growth, proliferation, and others 112 , 113 . However, any fluctuation of subcellular microenvironments can result in various disorders.…”

Section: Subcellular Compartment Sers Detectionsmentioning

confidence: 99%

Recent progress of surface-enhanced Raman spectroscopy for subcellular compartment analysis

Shen

Yue

et al. 2021

Theranostics

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Organelles are involved in many cell life activities, and their metabolic or functional disorders are closely related to apoptosis, neurodegenerative diseases, cardiovascular diseases, and the development and metastasis of cancers. The explorations of subcellular structures, microenvironments, and their abnormal conditions are conducive to a deeper understanding of many pathological mechanisms, which are expected to achieve the early diagnosis and the effective therapy of diseases. Organelles are also the targeted locations of drugs, and they play significant roles in many targeting therapeutic strategies. Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical tool that can provide the molecular fingerprint information of subcellular compartments and the real-time cellular dynamics in a non-invasive and non-destructive way. This review aims to summarize the recent advances of SERS studies on subcellular compartments, including five parts. The introductions of SERS and subcellular compartments are given. SERS is promising in subcellular compartment studies due to its molecular specificity and high sensitivity, and both of which highly match the high demands of cellular/subcellular investigations. Intracellular SERS is mainly cataloged as the labeling and label-free methods. For subcellular targeted detections and therapies, how to internalize plasmonic nanoparticles or nanostructure in the target locations is a key point. The subcellular compartment SERS detections, SERS measurements of isolated organelles, investigations of therapeutic mechanisms from subcellular compartments and microenvironments, and integration of SERS diagnosis and treatment are sequentially presented. A perspective view of the subcellular SERS studies is discussed from six aspects. This review provides a comprehensive overview of SERS applications in subcellular compartment researches, which will be a useful reference for designing the SERS-involved therapeutic systems.

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Nanostars on Nanopipette Tips: A Raman Probe for Quantifying Oxygen Levels in Hypoxic Single Cells and Tumours

Cited by 61 publications

References 54 publications

Scaffolds with a High Surface Area-to-Volume Ratio and Cultured Under Fast Flow Perfusion Result in Optimal O2 Delivery to the Cells in Artificial Bone Tissues

Scaffolds with a High Surface Area-to-Volume Ratio and Cultured Under Fast Flow Perfusion Result in Optimal O2 Delivery to the Cells in Artificial Bone Tissues

Vibrational Spectroscopic Estimation of Semivolatile Organic Compound Evaporation From Glass Surfaces

Recent progress of surface-enhanced Raman spectroscopy for subcellular compartment analysis

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