Nanostructure and mineral composition of trabecular bone in the lateral femoral neck: Implications for bone fragility in elderly women

Milovanović, Petar; Potočnik, Jelena; Stoiljković, Milovan; Djonić, Danijela; Nikolić, Slobodan; Nešković, O.; Djurić, Marija; Rakočević, Zlatko Lj.

doi:10.1016/j.actbio.2011.05.028

Cited by 43 publications

(48 citation statements)

References 40 publications

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“…6 information, bearing clear signs of increased tissue age with a person's aging. That is, when the mineral crystals stay "in place" undisturbed long enough, the processes of crystal growth and/or aggregation can take place, leading to an increased crystal size~Boskey, 2001; Milovanovic et al, 2011!. However, our AFM findings showed consistent bone nanostructural patterns at the external cortical surface in both age groups. Specifically, both in young and elderly women the investigated bone surface was composed of small densely packed mineral crystals that were similar to small mineral grains observed using the same in situ AFM technique in trabecular bone specimens of young women~Milovanovic et al, 2011!.…”

Section: Femoral Neck Outer Diametermentioning

confidence: 64%

“…Specifically, both in young and elderly women the investigated bone surface was composed of small densely packed mineral crystals that were similar to small mineral grains observed using the same in situ AFM technique in trabecular bone specimens of young women~Milovanovic et al, 2011!. Furthermore, several spectroscopy and microscopy bone studies demonstrated that smaller mineral crystals were a sign of newly deposited or more freshly remodeled bone~Eppell et al, 2001;Akkus et al, 2003;Su et al, 2003;Milovanovic et al, 2011!, whereas dominance of larger minerals was suggestive of older tissue age~Paschalis et al , 1997;Eppell et al, 2001;Milovanovic et al, 2011!. Our advanced nanostructural analyses~PSD and FD! showed similar surface texture and unchanged morphological complexity of the external cortical surfaces between young and elderly women, suggesting that both express similar tissue age~Milovanovic et al, 2012a!…”

Section: Femoral Neck Outer Diametermentioning

confidence: 91%

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Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging

et al. 2013

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On the basis of the suggestion that bone nanostructure bears “tissue age” information and may reflect surface deposition/modification processes, we performed nanoscale characterization of the external cortical bone surface at the femoral neck in women using atomic force microscopy (AFM). The specific aims were to assess age-related differences in bone nanostructure and explore the existence of nanostructural traces of potential bone apposition at this surface. Our findings revealed that the external cortical surface represents a continuous phase composed of densely packed mineral grains. Although the grains varied in size and shape, there was a domination of small grains indicative of freshly deposited bone (mean grain size: young, 35 nm; old, 37 nm; p > 0.05). Advanced quantitative analysis of surface morphological patterns revealed comparable roughness and complexity of the surface, suggesting a similar rate of mineral particle deposition at the surface in both groups. Calcium/phosphorus ratio, a measure of bone tissue age, was within the same range in both groups. In summary, our AFM analyses showed consistent nanostructural and compositional bone features, suggesting existence of new bone at the periosteal bone surface in both young and elderly women. Considering observed age-related increase in the neck diameter, AFM findings may support the theory of continuous bone apposition at the periosteal surface.

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Section: Femoral Neck Outer Diametermentioning

confidence: 64%

Section: Femoral Neck Outer Diametermentioning

confidence: 91%

Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging

et al. 2013

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“…The major advantage of the former is its non-invasive character, while in vitro studies frequently provide more accurate results due to the removal of fat, marrow, and collagen, which might obscure experimental measurements [18,19]. In vitro bone elemental analysis has been performed using synchrotron radiation micro-CT [20,21], neutron activation [22,23], X-ray diffraction [24], small-angle X-ray scattering [25,26], inductively coupled plasma optical emission spectroscopy (ICP-OES) combined with direct current argon arc plasma optical emission spectrometry (DCA ARC) [27], and chemical analysis [28]. Specifically related studies relying solely on spectroscopic methodologies include solid-state nuclear magnetic resonance (NMR) [29][30][31], X-ray fluorescence [32], TEM-EDX [33,34], SEM-EDX [35], and relevant surface analysis techniques such as X-ray photoelectron spectroscopy (XPS) [36,37] and AES [38].…”

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confidence: 99%

Ca/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy

2011

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Osteoporosis is a systemic skeletal disorder associated with reduced bone mineral density and the consequent high risk of bone fractures. Current practice relates osteoporosis largely with absolute mass loss. The assessment of variations in chemical composition in terms of the main elements comprising the bone mineral and its effect on the bone's quality is usually neglected. In this study, we evaluate the ratio of the main elements of bone mineral, calcium (Ca), and phosphorus (P), as a suitable in vitro biomarker for induced osteoporosis. The Ca/P concentration ratio was measured at different sites of normal and osteoporotic rabbit bones using two spectroscopic techniques: Auger electron spectroscopy (AES) and energy-dispersive X-ray spectroscopy (EDX). Results showed that there is no significant difference between samples from different genders or among cortical bone sites. On the contrary, we found that the Ca/P ratio of trabecular bone sections is comparable to cortical sections with induced osteoporosis. Ca/P ratio values are positively related to induced bone loss; furthermore, a different degree of correlation between Ca and P in cortical and trabecular bone is evident. This study also discusses the applicability of AES and EDX to the semiquantitative measurements of bone mineral's main elements along with the critical experimental parameters.

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“…The mineral inorganic phase is a form of poorly crystalline carbonated hydroxyapatite (Ca 10 [PO 4 ] 6 [OH] 2 ) incorporating several impurities [16,17] such as HPO 4 2− , CO 3 2− , Mg 2+ , Na + , F − and citrate, which are adsorbed onto the crystal surface and/or substituted in the lattice for Ca 2+ , PO 4 3− and OH − ions [18]. Impurities significantly increase solubility that is critical for phosphocalcic homeostasis and bone adaptation [19], while citrate stabilizes the apatite nanocrystals [20].…”

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confidence: 99%

Infrared spectroscopic assessment of the inflammation-mediated osteoporosis (IMO) model applied to rabbit bone

2012

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A model of osteoporosis based on induced inflammation (IMO) was applied to rabbit bones. The structural heterogeneity and molecular complexity of bone significantly affect bone mechanical properties. A tool like Fourier transform infrared spectroscopy, able to analyze both the inorganic and organic phase simultaneously, could provide compositional information regarding cortical and trabecular sections under normal and osteoporotic conditions. In this study, we assessed the mineral/matrix ratio, carbonate and phosphate content and labile (i.e., non-apatitic) species contribution to bone mineral and collagen cross-linking patterns. Clear differences were observed between cortical and trabecular bone regarding mineral and carbonate content. Induced inflammation lowers the mineral/matrix ratio and increases the overall carbonate accumulation. Elevated concentrations of labile species were detected in osteoporotic samples, especially in the trabecular sections. Collagen cross-linking patterns were indirectly observed through the 1660/1690 cm −1 ratio in the amide I band and a positive correlation was found with the mineralization index. Principal component analysis (PCA) applied to female samples successfully clustered trabecular and osteoporotic cases. The important role played by the phosphate ions was confirmed by corresponding loadings plots. The results suggest that the application of the IMO model to rabbit bones effectively alters bone remodeling and forms an osteoporotic bone matrix with a dissimilar composition compared to the normal one.

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Nanostructure and mineral composition of trabecular bone in the lateral femoral neck: Implications for bone fragility in elderly women

Cited by 43 publications

References 40 publications

Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging

Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging

Ca/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy

Infrared spectroscopic assessment of the inflammation-mediated osteoporosis (IMO) model applied to rabbit bone

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