Nanostructured Biomaterials for Regeneration

Wei, Guobao; Peter, X.

doi:10.1002/adfm.200800662

Cited by 259 publications

(168 citation statements)

References 106 publications

(142 reference statements)

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“…Nano-fibrous scaffolds can be obtained when the gelation of the polymer solution system occurs due to the crystallization of polymer-rich phase and solvent removal. Ma et al have done intensive studies on the fabrication of PLLA NF scaffolds by TIPS and their influence on cell morphology and function in bone tissue engineering (Woo et al, 2003;Hu et al, 2008;Smith et al, 2009;Wei and Ma, 2008). Even now, no one reported the application of PCL-b-PLLA scaffold with nano-fibrous structure fabricated by TIPS in cartilage tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that nanoporous or nano-fibrous polymer matrices played a critical role in providing support and anchorage for cells, while maintaining chondrocyte phenotype and enhancing chondrocyte growth and matrix synthesis Li et al, 2006). The fabrication technologies used for scaffold fabrication in cartilage tissue engineering included electrospinning (Wise et al, 2009), particulate leaching combined with chemical etching Park et al, 2005) and 3-D printing techniques (Yen et al, 2009). L-L TIPS is an easy and effective technique to fabricate 3D nano-fibrous scaffolds (Ma, 2008;Zhang and Ma, 1999).…”

Section: Discussionmentioning

confidence: 99%

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Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Liu²,

Guan³

et al. 2009

eCM

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Nano-fibrous scaffolds which could potentially mimic the architecture of extracellular matrix (ECM) have been considered a good candidate matrix for cell delivery in tissue engineering applications. In the present study, a semicrystalline diblock copolymer, poly(ε-caprolactone)-block-poly(L-lactide) (PCL-b-PLLA), was synthesized and utilized to fabricate nano-fibrous scaffolds via a thermally induced phase separation process. Uniform nano-fibrous networks were created by quenching a PCL-b-PLLA/THF homogenous solution to -20ºC or below, followed by further gelation for 2 hours due to the presence of PLLA and PCL microcrystals. However, knot-like structures as well as continuously smooth pellicles appeared among the nanofibrous network with increasing gelation temperature. DSC analysis indicated that the crystallization of PCL segments was interrupted by rigid PLLA segments, resulting in an amorphous phase at high gelation temperatures. Combining TIPS (thermally induced phase separation) with saltleaching methods, nano-fibrous architecture and interconnected pore structures (144±36 μm in diameter) with a high porosity were created for in vitro culture of chondrocytes. Specific surface area and protein adsorption on the surface of the nano-fibrous scaffold were three times higher than on the surface of the solid-walled scaffold. Chondrocytes cultured on the nano-fibrous scaffold exhibited a spherical condrocyte-like phenotype and secreted more cartilage-like extracellular matrix (ECM) than those cultured on the solid-walled scaffold. Moreover, the protein and DNA contents of cells cultured on the nanofibrous scaffold were 1.2-1.4 times higher than those on the solid-walled scaffold. Higher expression levels of collagen II and aggrecan mRNA were induced on the nanofibrous scaffold compared to on the solid-walled scaffold. These findings demonstrated that scaffolds with a nanofibrous architecture could serve as superior scaffolds for cartilage tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Liu²,

Guan³

et al. 2009

eCM

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“…Human bones are made up of 35 % organic matter, mainly collagen type I, and 65 % inorganic substances, where calcium phosphate predominates. In view of this, beside the polymer forming the fibrous frame, advanced bone substitutes include hydroxyapatite, which strengthens the structural integrity of the substitute and enables good osteoconductivity and binding of the natural bone components on the substitute (30). In vitro studies have confirmed a high degree of osteoblast attachment and growth on nanofibers from chitosan and hydroxyapatite as well as a significantly increased mineralization as soon as in 15 days (31).…”

Section: Nanofibers As Advanced Bone Substitutesmentioning

confidence: 88%

Nanofibers and their biomedical use

Rošic¹,

Kocbek²,

Pelipenko³

et al. 2013

Acta Pharmaceutica

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The idea of creating replacement for damaged or diseased tissue, which will mimic the physiological conditions and simultaneously promote regeneration by patients’ own cells, has been a major challenge in the biomedicine for more than a decade. Therefore, nanofibers are a promising solution to address these challenges. These are solid polymer fibers with nanosized diameter, which show improved properties compared to the materials of larger dimensions or forms and therefore cause different biological responses. On the nanometric level, nanofibers provide a biomimetic environment, on the micrometric scale three-dimensional architecture with the desired surface properties regarding the intended application within the body, while on the macrometric scale mechanical strength and physiological acceptability. In the review, the development of nanofibers as tissue scaffolds, modern wound dressings for chronic wound therapy and drug delivery systems is highlighted. Research substantiates the effectiveness of nanofibers for enhanced tissue regeneration, but ascertains that evidences from clinical studies are currently lacking. Nevertheless, due to the development of nano- and bio-sciences, products on the market can be expected in the near future.

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“…Alignment of polymer backbone structures along fiber axes forms crystal domains [150][151][152], which have provided a foundation for optoelectronics such as nanofiber light sources with color tunability and waveguide capabilities and nanofiber lasers [39,146,148,. Biologically, hybrid nanofiber scaffolds have provided a cell niche conducive to improved attachment, proliferation, and cell differentiation [190][191][192][193][194][195][196][197][198], as well as targeted drug delivery carriers [198][199][200][201][202][203][204][205][206]. Filtration using ONFs [207] equipped with activated carbon has revealed adsorption of volatile organic compounds (VOCs) present in the air [208][209][210], and Scholten et al reported that adsorption and desorption of VOC by electrospun nanofibrous membranes (ENMs) were faster than that of conventional activated carbon [208].…”

Section: Organic Micro-and Nano-fiber Systemsmentioning

confidence: 99%

Electrospinning for nano- to mesoscale photonic structures

et al. 2016

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Abstract:The fabrication of photonic and electronic structures and devices has directed the manufacturing industry for the last 50 years. Currently, the majority of small-scale photonic devices are created by traditional microfabrication techniques that create features by processes such as lithography and electron or ion beam direct writing. Microfabrication techniques are often expensive and slow. In contrast, the use of electrospinning (ES) in the fabrication of microand nano-scale devices for the manipulation of photons and electrons provides a relatively simple and economic viable alternative. ES involves the delivery of a polymer solution to a capillary held at a high voltage relative to the fiber deposition surface. Electrostatic force developed between the collection plate and the polymer promotes fiber deposition onto the collection plate. Issues with ES fabrication exist primarily due to an instability region that exists between the capillary and collection plate and is characterized by chaotic motion of the depositing polymer fiber. Material limitations to ES also exist; not all polymers of interest are amenable to the ES process due to process dependencies on molecular weight and chain entanglement or incompatibility with other polymers and overall process compatibility. Passive and active electronic and photonic fibers fabricated through the ES have great potential for use in light generation and collection in optical and electronic structures/ devices. ES produces fiber devices that can be combined with inorganic, metallic, biological, or organic materials for novel device design. Synergistic material selection and postprocessing techniques are also utilized for broad-ranging applications of organic nanofibers that span from biological to electronic, photovoltaic, or photonic. As the ability to electrospin optically and/or electronically active materials in a controlled manner continues to improve, the complexity and diversity of devices fabricated from this process can be expected to grow rapidly and provide an alternative to traditional resource-intensive fabrication techniques.

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Nanostructured Biomaterials for Regeneration

Cited by 259 publications

References 106 publications

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Nanofibers and their biomedical use

Electrospinning for nano- to mesoscale photonic structures

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