Nanostructured calcium phosphates (NanoCaPs) for non-viral gene delivery: Influence of the synthesis parameters on transfection efficiency

Olton, Dana; Li, Jinhua; Wilson, Mary E.; Rogers, Todd; Close, John M.; Huang, Leaf; Kumta, Prashant N.; Sfeir, Charles

doi:10.1016/j.biomaterials.2006.10.026

Cited by 247 publications

(216 citation statements)

References 38 publications

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“…Calcium phosphates (CaP) have been deposited onto polymer scaffolds for bone tissue engineering, and separately have been used as transfection reagents [108,109]. For immobilization, plasmid is co-precipitated with CaP as nanocrystalline CaP phases onto a surface [110].…”

Section: Vector Immobilizationmentioning

confidence: 99%

“…For immobilization, plasmid is co-precipitated with CaP as nanocrystalline CaP phases onto a surface [110]. The CaP vector is relatively non-toxic to cells, and the levels of transgene expression depend on the Ca/P ratio (maximal expression at ratio of 100-300) and the mixing conditions [109]. In the maximal expression conditions, 90% of the plasmid was bound to CaP, while 78% of the CaP bound plasmid actually resulted in complexed DNA in a size ranging between 25 and 50 nm.…”

Section: Vector Immobilizationmentioning

confidence: 99%

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Matrices and scaffolds for DNA delivery in tissue engineering

Laporte¹,

Shea²

2007

Advanced Drug Delivery Reviews

241

208

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Regenerative medicine aims to create functional tissue replacements, typically through creating a controlled environment that promotes and directs the differentiation of stem or progenitor cells, either endogenous or transplanted. Scaffolds serve a central role in many strategies by providing the means to control the local environment. Gene delivery from the scaffold represents a versatile approach to manipulating the local environment for directing cell function. Research at the interface of biomaterials, gene therapy, and drug delivery has identified several design parameters for the vector and the biomaterial scaffold that must be satisfied. Progress has been made towards achieving gene delivery within a tissue engineering scaffold, though the design principles for the materials and vectors that produce efficient delivery require further development. Nevertheless, these advances in obtaining transgene expression with the scaffold have created opportunities to develop greater control of either delivery or expression and to identify the best practices for promoting tissue formation. Strategies to achieve controlled localized expression within the tissue engineering scaffold will have broad application to the regeneration of many tissues, with great promise for clinical therapies.

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Section: Vector Immobilizationmentioning

confidence: 99%

Section: Vector Immobilizationmentioning

confidence: 99%

Matrices and scaffolds for DNA delivery in tissue engineering

Laporte¹,

Shea²

2007

Advanced Drug Delivery Reviews

241

208

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“…9 More recently, HAp nanoparticles (NPs) have been used as non-viral gene carriers. [10][11][12][13] Thus, DNA-HAp NPs complexes were found to cross cell membranes, integrating into the cell genome. In a very recent study, Brundin et al 14 showed a specific binding affinity of HAp for DNA.…”

Section: Introductionmentioning

confidence: 99%

Mineralization of DNA into nanoparticles of hydroxyapatite

et al. 2014

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“…[11] Recently, more efforts have been made to explore the potential of using CAP nanoparticles as vehicles for drug and gene delivery for their great affinity to DNA and various drugs and good release property. [12][13][14][15][16] Ceramics were also investigated for the adsorption of proteins, [17] delivery of hemoglobin, [18] insulin, [19] enzymes, [20,21] antigens, [22,23] and vaccines. [24,25] Different strategies were tested to fortify the characteristics of ceramics.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Vengala¹

2017

AJP

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Introduction: To enhance the delivery of poorly-soluble drugs, we have explored aquasomes (three-layered, ceramic core based, and oligosaccharide-coated nanoparticles) as potential carriers for the delivery of model hydrophobic drug lornoxicam (log P = 3.15). Materials and Methods: Ceramic nanoparticles were prepared using coprecipitation by sonication method. Cellobiose was used for coating onto ceramic core followed by loading of the lornoxicam by partial adsorption mechanism. The prepared system was characterized for size, shape, drug loading efficiency, and in vitro release profile (both 0.1 N hydrochloric acid solution and phosphate buffer solution, pH 6.8). Colorimetric analysis of sugar coating was done using phenol sulfuric acid method. Results and Discussion: The formed particles were spherical with an average particle size in the range of 60-300 nm, with a media of 87 nm. The in vitro dissolution performance was compared with that of pure drug and better results were observed. The cumulative lornoxicam release for the aquasome formulation (49%) was found to be higher than that of pure drug (34%) and was found to be gradual and linear in acidic media. Whereas, in phosphate buffer solution, pH 6.8, an incomplete release was observed with the pure drug (51% in 2 h) and 95% release was observed within 90 min from the formulation. Conclusion: Ceramic nanoparticles can be used for the enhancement of dissolution profile of poorly soluble drugs.

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Nanostructured calcium phosphates (NanoCaPs) for non-viral gene delivery: Influence of the synthesis parameters on transfection efficiency

Cited by 247 publications

References 38 publications

Matrices and scaffolds for DNA delivery in tissue engineering

Matrices and scaffolds for DNA delivery in tissue engineering

Mineralization of DNA into nanoparticles of hydroxyapatite

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