Nanostructured electrochemical DNA biosensors for detection of the effect of berberine on DNA from cancer cells

Abstract

Multi walled carbon nanotubes (MWNT) in dimethylformamide (DMF) or aqueous sodium dodecyl sulfate (SDS) solution, colloidal gold nanoparticles (GNP) in phosphate buffer solution (PBS), and a GNP-MWNT mixture in aqueous SDS solution have been investigated for chemical modification of a screen-printed carbon electrode used as the signal transducer of a dsDNA-based biosensor. Differential pulse voltammetry of the DNA redox marker Co[(phen)3]3+ and the guanine moiety anodic oxidation and cyclic voltammetry with K3[Fe(CN)6] as indicator revealed substantial enhancement of the response of the biosensor, particularly when MWNT in SDS solution was used. The biosensor was used in testing of berberine, an isoquinoline plant alkaloid with significant antimicrobial and anticancer activity. Berberine had a very strong, concentration-dependent, effect on the structural stability of DNA from the human cancer cells (U937 cells) whereas non-cancer cells were changed only when berberine concentrations were relatively high 75 and 50 microg mL(-1).

Disposable Electrochemical Biosensor with Multiwalled Carbon Nanotubes-Chitosan Composite Layer for the Detection of Deep DNA Damage

“…18 In the case of DNA modified electrode, the redox probe response is improved with the degradation of the negatively charged DNA backbone. 19 Electrochemical impedance spectroscopy (EIS) as another general method reflecting the electrical or structural properties of surfaces was also utilized for investigations of the immobilized DNA layer 20 as well as other biocomponents. 21 The aim of this work is to develop a cheap solid DNA sensor together with simple measurement procedures with a good ratio of specific-to-nonspecific signals.…”

Electrochemical Biosensors for Drug Analysis

“…where [Drug] is the concentration of a drug and I DNA-Drug and I DNA are the oxidative DNA guanine oxidation current in the presence and the absence of the studied drug, respectively [151].Using this methodology, the effect of berberine on the structural stability of DNA from human cancer and noncancer cells was compared [155]. The guanine functionalities are electrooxidized at relatively high positive potentials at carbon-based electrodes, i.e., close to water oxidation, and current trends are oriented towards the use of boron-doped diamond electrodes for DNA biosensors since such electrodes offer an extended positive potential domain of investigation [156].…”

Sensitively Electrochemical Sensing for Sequence‐Specific Detection of Phosphinothricin Acetyltransferase Gene: Layer‐by‐Layer Films of Poly‐L‐Lysine and Au‐Carbon Nanotube Hybrid

“…The hybridization events of target oligonucleotides were monitored by electrochemical impedance spectroscopic (EIS), cyclic voltammetric (CV) and alternating current voltammetric techniques. Ovadekova et al [27] prepared a nanostructured electrochemical DNA biosensor based on Au-CNT hybrid, which was used in testing of berberine, an isoquinoline plant alkaloid with significant antimicrobial and anticancer activity. However, there is a lack of report on the study and analytical performance of Au À CNT hybrid LBL self-assembly.…”