Nanostructured fibrin-agarose hydrogels loaded with allogeneic fibroblasts as bio-dressings for acute treatment of massive burns

Arribas-Arribas, Blanca; Fernández-Muñoz, Beatriz; Campos-Cuerva, Rafael; Montiel-Aguilera, Miguel Ángel; Bermejo-González, María; Lomas-Romero, Isabel; Martín-López, María; Alcázar-Caballero, Rosario Mata; del Mar Macías-Sánchez, María; Campos, Fernando; Alaminos, Miguel; Gómez-Cía, Tomás; Gacto, Purificación; Carmona, Gloria; Santos-González, Mónica

doi:10.1016/j.biopha.2023.115769

Cited by 2 publications

(1 citation statement)

References 32 publications

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“…Our previous work evaluated the e cacy of a novel hemostatic agent made from a nanostructured brin and type VII agarose hydrogel (NFAH) [9]. NFAH has previously been used as a scaffold biomaterial in several preclinica [10], [11], [12] and clinical [13], [14]. This brin and agarose matrix is mainly derived from human plasma, has high exibility, elasticity and mechanical strength [15], [16] and has been proven to be biodegradable and biocompatible [11], [17].…”

Section: Introductionmentioning

confidence: 99%

Cryopreserved nanostructured fibrin-agarose hydrogels are efficient and safe hemostatic agents

Casado,

Cepeda-Franco,

Arenas

et al. 2024

Preprint

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Uncontrolled bleeding during surgery is associated with high mortality and prolonged hospital stay, necessitating the use of hemostatic agents. Fibrin sealant patches offer an efficient solution to achieve hemostasis and improve patient outcomes in liver resection surgery. We have previously demonstrated the efficacy of a nanostructured fibrin-agarose hydrogel (NFAH). However, for the widespread distribution and commercialization of the product, it is necessary to develop an optimal preservation method that allows for prolonged stability and facilitates storage and distribution. We investigated cryopreservation as a potential method for preserving NFAH using trehalose. Structural changes in cryopreserved NFAH (Cryo-NFAH) were investigated and comparative in vitro and in vivo efficacy and safety studies were performed with freshly prepared NFAH. We also examined the long-term safety of Cryo-NFAH versus TachoSil® in a rat partial hepatectomy model, including time to hemostasis, intra-abdominal adhesion, hepatic hematoma, inflammatory factors, histopathological variables, temperature and body weight, hemocompatibility and cytotoxicity. Structural analyses demonstrated that Cryo-NFAH retained most of its macro- and microscopic properties after cryopreservation. Likewise, hemostatic efficacy assays showed no significant differences with fresh NFAH. Safety evaluations indicated that Cryo-NFAH had a similar overall profile to TachoSil® up to 40 days post-surgery in rats. In addition, Cryo-NFAH demonstrated superior hemostatic efficacy compared with TachoSil® while also demonstrating lower levels of erythrolysis and cytotoxicity than both TachoSil® and other commercially available hemostatic agents. These results indicate that Cryo-NFAH is highly effective hemostatic patch with a favorable safety and tolerability profile, supporting its potential for clinical use.

show abstract

Section: Introductionmentioning

confidence: 99%

Cryopreserved nanostructured fibrin-agarose hydrogels are efficient and safe hemostatic agents

Casado,

Cepeda-Franco,

Arenas

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Cryopreserved nanostructured fibrin-agarose hydrogels are efficient and safe hemostatic agents

Casado,

Cepeda-Franco,

Pereira Arenas

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Uncontrolled bleeding during surgery is associated with high mortality and prolonged hospital stay, necessitating the use of hemostatic agents. Fibrin sealant patches offer an efficient solution to achieve hemostasis and improve patient outcomes in liver resection surgery. We have previously demonstrated the efficacy of a nanostructured fibrin-agarose hydrogel (NFAH). However, for the widespread distribution and commercialization of the product, it is necessary to develop an optimal preservation method that allows for prolonged stability and facilitates storage and distribution. We investigated cryopreservation as a potential method for preserving NFAH using trehalose. Structural changes in cryopreserved NFAH (Cryo-NFAH) were investigated and comparative in vitro and in vivo efficacy and safety studies were performed with freshly prepared NFAH. We also examined the long-term safety of Cryo-NFAH versus TachoSil in a rat partial hepatectomy model, including time to hemostasis, intra-abdominal adhesion, hepatic hematoma, inflammatory factors, histopathological variables, temperature and body weight, hemocompatibility and cytotoxicity. Structural analyses demonstrated that Cryo-NFAH retained most of its macro- and microscopic properties after cryopreservation. Likewise, hemostatic efficacy assays showed no significant differences with fresh NFAH. Safety evaluations indicated that Cryo-NFAH had a similar overall profile to TachoSil up to 40 days post-surgery in rats. In addition, Cryo-NFAH demonstrated superior hemostatic efficacy compared with TachoSil while also demonstrating lower levels of erythrolysis and cytotoxicity than both TachoSil and other commercially available hemostatic agents. These results indicate that Cryo-NFAH is highly effective hemostatic patch with a favorable safety and tolerability profile, supporting its potential for clinical use.

show abstract

Nanostructured fibrin-agarose hydrogels loaded with allogeneic fibroblasts as bio-dressings for acute treatment of massive burns

Cited by 2 publications

References 32 publications

Cryopreserved nanostructured fibrin-agarose hydrogels are efficient and safe hemostatic agents

Cryopreserved nanostructured fibrin-agarose hydrogels are efficient and safe hemostatic agents

Cryopreserved nanostructured fibrin-agarose hydrogels are efficient and safe hemostatic agents

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