Nanostructured poly(thiophene acetic acid)/Au/poly(methylene blue) interface for electrochemical immunosensing of p53 protein

Cruz-Pacheco, Andrés F.; Quinchia, Jennifer; Orozco, Jahir

doi:10.1007/s00604-023-05683-5

Cited by 11 publications

(6 citation statements)

References 39 publications

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“…To enhance the surface properties of biomolecule-covered surfaces, beneficial immobilization matrices are crucial in the design of sensing interfaces. 4,5 To fabricate immunosensors with high-quality sensing platforms, various materials such as natural 6 and synthetic polymers 7 and nanostructures such as gold nanoparticles, 8 quantum dots, 9 and carbon nanotubes 10 have been used as a support matrix for the immobilization of antibodies. Also, electrospun nanofibers (ENs) in immunosensor design have been attracting great attention nowadays because of their unique properties.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Novel materials and fabrication techniques provide the development of immunosensors with better performance characteristics. To enhance the surface properties of biomolecule-covered surfaces, beneficial immobilization matrices are crucial in the design of sensing interfaces. , To fabricate immunosensors with high-quality sensing platforms, various materials such as natural and synthetic polymers and nanostructures such as gold nanoparticles, quantum dots, and carbon nanotubes have been used as a support matrix for the immobilization of antibodies. Also, electrospun nanofibers (ENs) in immunosensor design have been attracting great attention nowadays because of their unique properties. , ENs may create a porous membrane structure with a high surface-to-volume ratio, which increases performance in a variety of applications. , The nanofiber structure, characteristics, and morphology are required for a variety of applications .…”

Section: Introductionmentioning

confidence: 99%

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Antibody-Conjugated Electrospun Nanofibers for Electrochemical Detection of Methamphetamine

Atik

Kilic

Horzum

et al. 2023

ACS Appl. Mater. Interfaces

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Multifunctional electrospun nanofibers (ENs) with improved properties have increased attention nowadays. Their insoluble forms in water with decreased hydrophobicity are desired for the immobilization of biological molecules. Also, the addition of functional groups on the backbone provides the conjugation of biomolecules onto the surface of ENs via covalent bonds to increase their stability. Here, poly(vinylidene fluoride) (PVDF) was chosen to prepare a platform, which is insoluble in water, and polyethylenimine (PEI) was used to add amine groups on the surface of ENs to bind biological molecules via covalent conjugation. So, PVDF-PEI nanofibers were prepared on a glassy carbon electrode to immobilize an antimethamphetamine antibody (Anti-METH) as a model biomolecule. The obtained PVDF-PEI/Anti-METH was used for the bioelectrochemical detection of methamphetamine (METH), a common illicit drug. Bioelectrochemical detection of METH on PVDF-PEI/Anti-METH-coated electrodes was carried out by voltammetry in the range of 2.0–50 ng/mL METH. Moreover, the effect of dansyl chloride (DNC) derivatization of METH on the sensitivity of PVDF-PEI/Anti-METH was tested. Finally, METH analysis was carried out in synthetic body fluids. The obtained results showed that PVDF-PEI ENs can be adopted as an immobilization matrix for the biorecognition elements of biobased detection systems, and the derivative of METH (METH-DNC) increased the sensitivity of PVDF-PEI/Anti-METH.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibody-Conjugated Electrospun Nanofibers for Electrochemical Detection of Methamphetamine

Atik

Kilic

Horzum

et al. 2023

ACS Appl. Mater. Interfaces

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“…Increased serum levels of the tumor suppressor protein p53 have become a promising biomarker for diagnosing CRC (Aydın et al, 2018). We electrochemically assembled stepby-step a poly(thiophene acetic acid)/Au/poly(methylene blue) nanostructured interface at SPCE for label-free detection of p53 protein (Figure 4D) (Cruz-Pacheco et al, 2023). The conductive properties of the polymeric interface increased with an additional layer of poly(methylene blue) electropolymerized in the presence of gold nanoparticles.…”

Section: Nanobiosensors For the Detection Of Cancer-related Biomarkersmentioning

confidence: 99%

Nanociencia, nanotecnología y tecnologías disruptivas en el contexto de la medicina de precisión

Orozco

2023

Rev. Acad. Colomb. Cienc. Ex. Fis. Nat.

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El cuidado de la salud está experimentando un cambio de paradigma con respecto al diagnóstico y manejo de enfermedades orientado a llevar la medicina y la atención personalizadas a quienes lo necesitan. En este sentido, las ciencias puras han encontrado aliados en la nanociencia, la nanotecnología y otras tecnologías convergentes y disruptivas para asumir tan exigente tarea. Aquí se ilustra cómo dicha convergencia puede generar soluciones sin precedentes en el cuidado de la salud, por ejemplo, con el uso de nanobiosensores electroquímicos para monitorear patógenos y biomarcadores multiparamétricos a diferentes niveles moleculares y dilucidar el estado de salud de un individuo de manera personalizada. Se presentan, asimismo, ejemplos de nanobiosensores electroquímicos explorados para el diagnóstico, su potencial para el pronóstico y la posible evaluación del riesgo de complicaciones en pacientes. En este contexto, los diagnósticos inteligentes juegan un papel crucial en el cuidado de la salud 4.0, ya que puede avanzarse desde los datos inteligentes hacia dispositivos de diagnóstico capaces de analizar macrodatos que se beneficien del Internet de las cosas, el aprendizaje automático, el aprendizaje profundo y los teléfonos inteligentes entre otras tecnologías convergentes y disruptivas de vanguardia. Además, el trabajo destaca el potencial de los nanoportadores funcionales para la administración controlada y específica de fármacos como una rama de la medicina personalizada que promete revolucionar la atención médica. Por último, se hacen algunas reflexiones sobre medicina traslacional, así como comentarios finales y el recuento de los desafíos que implica llevar masivamente esta nueva y revolucionaria tecnología a los sistemas de salud globales.

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“…PTAA is a macromolecule composed of π-conjugated organic monomers with delocated electrons, whose conductivity can be improved when doped with an electron donor (n-doping) or acceptor (pdoping). 29 After that, the sensing performance of the caffeine-based sensors was subsequently examined, and the caffeine-pine PTAA-based sensor had the highest performance in terms of detection range, sensitivity, and limit of detection. Artificial blood and brain samples from AD mice were afterward studied to demonstrate that the potential utility for early diagnosis of AD in vitro and ex vivo.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, compared with the self‐assembled MPA, the carboxylic acid‐modified PTAA 27 was selected to deposit on the electrode surface by electropolymerization and the residual COOH can support an immobilization of bioreceptors, which effectively improves the stability, adhesion, electrochemically activated surface area, and conductivity 28 (Figure 1). PTAA is a macromolecule composed of π‐conjugated organic monomers with delocated electrons, whose conductivity can be improved when doped with an electron donor (n‐doping) or acceptor (p‐doping) 29 . After that, the sensing performance of the caffeine‐based sensors was subsequently examined, and the caffeine–pine PTAA‐based sensor had the highest performance in terms of detection range, sensitivity, and limit of detection.…”

Section: Introductionmentioning

confidence: 99%

Portable non‐enzymatic electrochemical biosensor based on caffeine for Alzheimer's disease diagnosis

Zhang,

Wang,

Wang

et al. 2023

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Caffeine and its derivatives can effectively bind amyloid beta 16–22 (Aβ16–22) fragment of amyloid beta 1–42 (Aβ1–42), a biomarker for the early diagnosis of Alzheimer's disease (AD), by means of conformation selection, π–π stacking, van der Waals forces, and hydrogen bonding, so as to achieve high specificity and quantitative detection of Aβ1–42. In this study, 3‐mercaptopropionic acid (MPA), conductive polymer poly(thiophene‐3‐acetic acid) (PTAA), and pine‐like/Au pine PTAA (pine PTAA) were applied to modify the electrodes, and the non‐enzymatic caffeine was used as specific biorecognition element to study the analytical performance of the electrochemical sensor platform for Aβ1–42 oligomer (AβO). It was found that caffeine/pine PTAA‐based sensor with large surface area, high active sites, and excellent electrical conductivity demonstrated the widest linear range (10−8 to 100 nM) and highest sensitivity (743.77 Ω/log nM) in comparison. The prepared caffeine‐based sensor was afterward applied to cerebrospinal fluid and blood tests for real sample analysis, demonstrating its potential for practical use in detecting AβO at the attomolar level. Furthermore, the non‐enzymatic caffeine was constructed on the pine‐like PTAA‐modified screen‐printed electrodes for the rapid detection of AβO using portable meter.

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Nanostructured poly(thiophene acetic acid)/Au/poly(methylene blue) interface for electrochemical immunosensing of p53 protein

Cited by 11 publications

References 39 publications

Antibody-Conjugated Electrospun Nanofibers for Electrochemical Detection of Methamphetamine

Antibody-Conjugated Electrospun Nanofibers for Electrochemical Detection of Methamphetamine

Nanociencia, nanotecnología y tecnologías disruptivas en el contexto de la medicina de precisión

Portable non‐enzymatic electrochemical biosensor based on caffeine for Alzheimer's disease diagnosis

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