Nanostructured rough gold electrodes as platforms to enhance the sensitivity of electrochemical genosensors

García‐Mendiola, Tania; Gamero, M.; Campuzano, Susana; Revenga-Parra, Mónica; Alonso, C.; Pedrero, Marı́a; Pariente, F.; Pingarrón, José M.; Lorenzo, Encarnación

doi:10.1016/j.aca.2013.06.009

Cited by 18 publications

(8 citation statements)

References 38 publications

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“…Electrochemical treatment of the gold surface can produce extremely rough electrodes (R f =40) with a 10‐fold CP strands loading but they must be maintained in water at 4 °C to preserve the nanopattern prior to their use. Interestingly, after modification with the DNA SAM, the nanostructure is stable even at 40 °C temperature at which the hybridization was carried out .…”

Section: Influence Of the Topography Of The Gold Supportmentioning

confidence: 99%

Understanding the Factors Affecting the Analytical Performance of Sandwich‐hybridization Genosensors on Gold Electrodes

2018

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Hybridization‐based electrochemical DNA sensors have become a methodological mainstay in a wide variety of fields. Among the possible assay formats, sandwich hybridization is the most powerful one. It is so called because the target sequence to be measured is hybridized between two complementary sequences. The analytical performance of this class of sensors is influenced by many construction and operational parameters, which need to be optimized. In this work, we comparatively analyze the analytical performance of different sandwich hybridization sensors, constructed on gold surfaces modified by chemisorption. We find that the structures of the designed probes as well as the topography of the gold support and the nature of the monolayer are key parameters affecting the sensitivity and reproducibility of the genosensors. Other important operational parameters are also described. The results of this study could serve as a guide in the design of genosensors for future applications.

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Section: Influence Of the Topography Of The Gold Supportmentioning

confidence: 99%

Understanding the Factors Affecting the Analytical Performance of Sandwich‐hybridization Genosensors on Gold Electrodes

2018

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“…Later, the oxide layer is submitted to electroreduction under either a slow potential sweep or a potential step, in the same acid solution [25]. The resulting nanopatterning produces rough electrodes with a large active surface area, which can be favorably exploited for the preparation of bioelectrodes with improved analytical performance [26].…”

Section: Metal Nanoparticlesmentioning

confidence: 99%

“…The optimal performances displayed by the lactate biosensor suggested to other researchers that rough Au electrode surfaces can be considered as promising electrochemical transducers for the production of other bioanalytical platforms. In fact, DNA biosensors based on nanostructured Au electrodes have been fabricated for the detection of synthetic short DNA sequences [47], typical genes fragments and bacterial DNA [26].…”

Section: Metal Nanoparticlesmentioning

confidence: 99%

Electrochemical Deposition of Nanomaterials for Electrochemical Sensing

Tonelli¹,

Scavetta²,

Gualandi³

2019

Sensors

156

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The most commonly used methods to electrodeposit nanomaterials on conductive supports or to obtain electrosynthesis nanomaterials are described. Au, layered double hydroxides (LDHs), metal oxides, and polymers are the classes of compounds taken into account. The electrochemical approach for the synthesis allows one to obtain nanostructures with well-defined morphologies, even without the use of a template, and of variable sizes simply by controlling the experimental synthesis conditions. In fact, parameters such as current density, applied potential (constant, pulsed or ramp) and duration of the synthesis play a key role in determining the shape and size of the resulting nanostructures. This review aims to describe the most recent applications in the field of electrochemical sensors of the considered nanomaterials and special attention is devoted to the analytical figures of merit of the devices.

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“…Instead of rather nanostructured, rough gold electrodes (García-Mendiola et al 2013) or randomly nanoporous gold surfaces (Patel et al 2013), anodic alumina oxide templates are an attractive approach to fabricate various nanostructures with excellent regularity and the ability to tune their sizes and lengths (Wang, Min, and Zhu 2013;Wu, Zhang, and Du 2013). The construction using anodic alumina oxide is less complicated, less expensive, and less timeconsuming when compared to traditional lithographic approach or epitaxial growth of highly ordered arrays of nanostructures.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanostructured Surface for Electrochemical Sensing and Biosensing: Does Shape Matter?

et al. 2015

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The construction of an electrochemical sensor based on a gold working electrode modified with gold nanostructures was achieved by galvanic deposition on an anodic alumina nanoporous template. Two approaches were investigated to fabricate the nanostructures of various sizes and Downloaded by [University of Nebraska, Lincoln] at 03:13 05 October 2015 3 shapes. First, gold deposition was performed through a thin alumina template resulting in the production of nanorods. In the second approach, alumina pores were widened to form cupshaped nanostructures. The detailed topography of the nanostructured surfaces was characterized by scanning electron microscopy. The lengths and the diameters of the nanostructures were anticipated to have a significant influence on the surface area and thus on the capacity to promote electron-transfer reactions. This dependence was investigated by electrochemical impedance spectroscopy and cyclic voltammetry. The functionalization of electrode surface with 11-mercaptoundecanoic acid provided a basis for further bonding with analytes. A modified Randles equivalent circuit model with a constant phase element was used to interpret the impedance spectra. The interfacial properties of the modified electrodes were also evaluated by cyclic voltammetry in the presence of the 3 /4 6 Fe(CN)  redox couple as a probe. The results showed that the voltammetric behavior of the probe was influenced by surface modification. The accumulation of 11-mercaptoundecanoic acid on the nanostructured electrode surface did not result in the expected increase in charge-transfer resistance and the decreased capacitance, unlike the cup-shaped nanostructured electrode.

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Nanostructured rough gold electrodes as platforms to enhance the sensitivity of electrochemical genosensors

Cited by 18 publications

References 38 publications

Understanding the Factors Affecting the Analytical Performance of Sandwich‐hybridization Genosensors on Gold Electrodes

Understanding the Factors Affecting the Analytical Performance of Sandwich‐hybridization Genosensors on Gold Electrodes

Electrochemical Deposition of Nanomaterials for Electrochemical Sensing

Gold Nanostructured Surface for Electrochemical Sensing and Biosensing: Does Shape Matter?

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