Nanostructured ZnO-based biosensor: DNA immobilization and hybridization

Mohammed, Ahmed Mishaal; Ibraheem, Ibraheem J.; Obaid, A.S.; Bououdina, M.

doi:10.1016/j.sbsr.2017.07.003

Cited by 50 publications

(18 citation statements)

References 41 publications

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“…The hybrid structure based on nickel-ZnO film was investigated to biosensor fabrication for the meningitis DNA diagnosis, [15]. A DNA biosensor was materialized using APTES with ZnO NSs fabricated via chemical vapor deposition (CVD) growth mechanism for electrochemical biosensor approach and purpose, [16].…”

Section: Introductionmentioning

confidence: 99%

The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA detection based on tellurium doped ZnO nanowires

et al. 2019

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The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA (HPV DNA) detection based on tellurium doped ZnO nanowires was fabricated. The NWs were grown by hybrid thin film oxidation in the physical vapor deposition (PVD) mechanism. The morphology characterization of the synthesized NWs was performed by field emission scanning electron microscopy (FESEM) and the images demonstrated that the diameter and the length of the materialized NWs were around 50 nm and several micrometers, respectively. The high-resolution transmission electron microscopy (HRTEM) image indicated that the fabricated NWs were crystalline and their phase characterization was validated by the X-ray diffraction pattern (XRD pattern). The single-stranded DNA (ss DNA) probe was immobilized on the surface of the Te-ZnO NWs. The electrochemical impedance spectra (EIS) measurements showed high response sensitivity after hybridization with complementary oligonucleotides. The biosensor could distinguish complementary target from non-complementary and mismatch oligonucleotides. The HBV biosensor could respond to complementary target in the concentrations range from 1 pM to 1 μM. The limit of detection (LOD) of the biosensor was 0.1 pM. The stability of the HBV DNA biosensor was investigated and biosensor could show 95% of its initial responses after 8 weeks maintenance.

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Section: Introductionmentioning

confidence: 99%

The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA detection based on tellurium doped ZnO nanowires

et al. 2019

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“…Mohammed et al. verified the hybridization of complementary target DNA to probe DNA by observing changes in the electrochemical properties of ZnO nanorod . ZnO nanorods were prepared by means of a chemical bath deposition (CBD) method.…”

Section: Electrochemical‐based Biosensorsmentioning

confidence: 99%

“…Mohammed et al verified the hybridization of complementary target DNA to probe DNA by observing changes in the electrochemical properties of ZnO nanorod. [170] ZnO nanorods were prepared by meanso fachemical bath deposition (CBD) method.T he CBD methodi nvolves the formation of solid nanomaterials on as ubstrate in solution. CBD is composed of two steps:n ucleation and particle growth.…”

Section: Zno As An Electrochemicalb Iosensormentioning

confidence: 99%

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

et al. 2019

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Label‐free biosensors offer accurate sensing capabilities due to the reliable quantification of biological and biochemical processes. These devices function by establishing a dynamic interaction of analyte and receptor molecules and convert this interaction into a measurable signal through a transducer. In recent decades, label‐free biosensors have attracted attention in biomedical applications due to the ease of linking nanomaterials with bioreceptor molecules. In this review, recent advances in sensitivity, specificity, and sensing mechanism related to label‐free biosensors of metallic nanoparticles of gold, silver, aluminium, copper, and zinc oxide are presented. Selected sensing methods based on fluorescence, surface plasmon resonance, surface‐enhanced Raman scattering, metal‐enhanced fluorescence, and electrochemical sensors are discussed. New measurement techniques and rapid progress of label‐free biosensors are going to play a vital role in the real‐time detection of biomarkers in clinical samples, such as blood plasma, serum, and urine, as well as in targeted drug delivery. Future trends of these label‐free biosensing mechanisms and their development are also discussed.

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“…Zinc oxide and copper oxide nanostructures have been already using in different types of gas [39,40], heavy metal ions [39,[41][42][43] and bio-sensors [44][45][46][47][48] The formation of CuO nanostructures occurs according to the dissolutionsecondary precipitation mechanism in two stages, when copper hydroxide is formed at the beginning of the growth process, followed by its oxidation. The growth mechanism is described in the articles [49][50][51][52].…”

Section: Introductionmentioning

confidence: 99%

Nanostructure-based electrochemical sensor: glyphosate detection and the analysis of genetic changes in rye DNA

Gerbreders

Krasovska

Mihailova

et al. 2019

Preprint

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Glyphosate, commonly known by its original trade name Roundup™, is the world’s most widely used herbicide. Glyphosate and it metabolites have profound negative environmental impact and long-term toxicity risk including cancerogenity, genotoxicity, endocrine disruption even at concentration levels too low to have a herbicidal effect. Therefore, the detection of these pollutants in low concentrations is an actual and important task. To increase the sensitivity of the sensor, nanostructures were used. To analyze the presence of glyphosate and its metabolites in rye juice, two groups of samples were selected. In the first case, glyphosate at different concentrations was added to the water for irrigation on the first day and then the samples were watered with pure water for 7 days. In the second case, rye was watered with pure water for all 8 days, and glyphosate was artificially added just before the measurement. The obtained samples were studied by the DPV employing nanostructured working electrode. To analyze changes in the DNA sequence, a PCR product obtained from samples of the first group was electrochemically studied. To confirm the results obtained, an electrophoresis method was also applied. The results indicate that the DPV signal obtained from samples with artificially added glyphosate has significant differences compared to the signal obtained from the juice of plants absorbing glyphosate in a natural way during growth. However, in both cases, CuO nanostructure based sensor detects the presence of glyphosate or its metabolites compared with the control sample. The experiment also found significant changes in the DNA caused by exposure with glyphosate during rye growth process of rye sprouts.

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Nanostructured ZnO-based biosensor: DNA immobilization and hybridization

Cited by 50 publications

References 41 publications

The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA detection based on tellurium doped ZnO nanowires

The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA detection based on tellurium doped ZnO nanowires

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

Nanostructure-based electrochemical sensor: glyphosate detection and the analysis of genetic changes in rye DNA

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