Nanostructures for peroxidases

Abstract

Peroxidases are enzymes catalyzing redox reactions that cleave peroxides. Their active redox centers have heme, cysteine thiols, selenium, manganese, and other chemical moieties. Peroxidases and their mimetic systems have several technological and biomedical applications such as environment protection, energy production, bioremediation, sensors and immunoassays design, and drug delivery devices. The combination of peroxidases or systems with peroxidase-like activity with nanostructures such as nanoparticles, nanotubes, thin films, liposomes, micelles, nanoflowers, nanorods and others is often an efficient strategy to improve catalytic activity, targeting, and reusability.

A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

“…Inspiring from Zare and coworkers, several research groups studied the preparation and characterization of organic-inorganic hybrid nanoflowers using different enzymes and metal ions for different purposes [42][43][44][45][46][47][48][49]. In one of our study, we determined how the experimental parameters, such as concentrations of copper (Cu 2+ ) ions, chloride ions (Cl -) and HRP enzyme; the pH of the buffer solution (phosphate buffered saline); and the temperature of the reaction, affect the morphology and activity performance of synthesized nanoflowers.…”

Current state of a dual behaviour of antimicrobial peptides—Therapeutic agents and promising delivery vectors

“…[34,62] The AMPs' cargo has to reach the intracellular bacteria, which are usually found in the phagocytic cells. [63] However, AMPs display a relatively low stability in vivo due to the interactions with body fluid anionic species, [64] resulting in a reduction in the local concentration of active substances and a decrease in their activity. AMPs, as amphiphilic and positively charged molecules, are able to adsorb serum proteins.…”

Technological Applications of Porphyrins and Related Compounds: Spintronics and Micro-/Nanomotors

“…The remarkable chemical and photophysical properties of porphyrins have attracted the interest of researchers worldwide [1]. Biological and technological applications of porphyrins can involve the use of native hemeproteins, metallo-substituted hemeproteins, and the product of the tryptic digestion of horse heart cytochrome c, microperoxidases [11][12][13][14][15][16][17][18][19]. Inspired by nature, researchers have synthesized a diversity of nonnatural porphyrins.…”

“…Chiral molecules act as a spin filter in the electron transfer favoring the production of spin-parallel hydroxyl free radicals and consequently oxygen evolution simultaneously with H 2 production [28]. In the literature, the association of porphyrins and/or hemeproteins with nanostructures, especially for photodynamic therapy purposes, is reported [14,29]. The reason for this association refers to an enormous quantity of studies and recent findings involving nanostructure properties and manipulation, particularly the potential for drug delivery systems [30].…”