Nanotechnology based activation-immobilization of psychrophilic pectate lyase: A novel approach towards enzyme stabilization and enhanced activity

Mukhopadhyay, Arka; Bhattacharyya, Tamoghna; Dasgupta, Anjan Kr.; Chakrabarti, Krishanu

doi:10.1016/j.molcatb.2015.05.017

Cited by 18 publications

(9 citation statements)

References 38 publications

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“…Mukhopadhyay et al showed that bacterial mesophilic laccase [64] treated with Cu 2 O-NPs retains its secondary structure even at temperature of ~ 80-90°C while the untreated one became disrupted (reflected in a substantial decrease of its β -sheet content). Almost an identical result was found in case of a protease, wherein, hydroxyl-apatite NP helped to retain its secondary structure at higher temperatures [64–68] (Table 3).…”

Section: Impact To the Biology Of The Targeted Systemsupporting

confidence: 64%

Inner-View of Nanomaterial Incited Protein Conformational Changes: Insights into Designable Interaction

2018

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Nanoparticle bioreactivity critically depends upon interaction between proteins and nanomaterials (NM). The formation of the “protein corona” (PC) is the effect of such nanoprotein interactions. PC has a wide usage in pharmaceuticals, drug delivery, medicine, and industrial biotechnology. Therefore, a detailed in-vitro, in-vivo, and in-silico understanding of nanoprotein interaction is fundamental and has a genuine contemporary appeal. NM surfaces can modify the protein conformation during interaction, or NMs themselves can lead to self-aggregations. Both phenomena can change the whole downstream bioreactivity of the concerned nanosystem. The main aim of this review is to understand the mechanistic view of NM-protein interaction and recapitulate the underlying physical chemistry behind the formation of such complicated macromolecular assemblies, to provide a critical overview of the different models describing NM induced structural and functional modification of proteins. The review also attempts to point out the current limitation in understanding the field and highlights the future scopes, involving a plausible proposition of how artificial intelligence could be aided to explore such systems for the prediction and directed design of the desired NM-protein interactions.

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Section: Impact To the Biology Of The Targeted Systemsupporting

confidence: 64%

Inner-View of Nanomaterial Incited Protein Conformational Changes: Insights into Designable Interaction

2018

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“…Here is the example of Pectate lyase (PL) in which formerly Ca nanoparticles were used in the immobilization procedure but then for the improvement of PL stability Ca nanoparticles were replaced by Calcium Hydroxyapatite and SWNT were used for its entrapment, this replacement was fruitful as it doesn't only stabilize enzyme at low temperature but also at high temperature. As the enzyme is psychrophilic in nature so it was stable only at low temperature but at high temperature it remains stable by maintaining its activity [36].…”

Section: Nanometalsmentioning

confidence: 99%

Use of Nanomaterials for the Immobilization of Industrially Important Enzymes

Fatima¹

2021

Preprint

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Immobilization enables enzymes to be held in place so that they can be easily separated from the product when needed and can be used again. Conventional methods of immobilization include adsorption, encapsulation, entrapment, cross linking and covalent binding. However, conventional methods have several drawbacks including reduced stability, loss of biomolecules, less enzyme loading or activity and limited diffusion. The aim of this study is the evaluation of importance of nanomaterials for the immobilization of industrially important enzymes. Nano materials are now in trend for the immobilization of different enzymes due to their physiochemical properties. Gold nanoparticles, silver nanoparticles, nano diamonds, graphene, carbon nanotubes and others are used for immobilization. Among covalent and non-covalent immobilization of enzymes involving both single and multiwalled carbon nanotubes, non-covalent immobilization with functionalized carbon nanotubes is superior. Therefore, enzymes immobilized with nanomaterials possess greater stability, retention of catalytic activity and reusability of enzymes

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“…Application of immobilized enzymes in industrial bioprocesses can offer several unique advantages over the soluble enzyme such as enzyme reusability, enhancement of enzyme stability, simplifying product purification and better bioprocess control, resulting in reduction of the bioprocess cost (Ibrahim et al, 2014 andMadhavan et al, 2017). Previously several solid supports have been used as carriers for enzyme immobilization; however as a result of recent progress in nanotechnology diverse nanostructured materials have been provided that potentially support the enzymes immobilization technology and opened a new field, the so-called nanobiocatalysis (Ansari & Husain, 2011;Raval et al, 2014 andMukhopadhyay et al, 2015).…”

Section: Recent Advance In Physical Stabilization Of Enzymementioning

confidence: 99%

Recent Trends for Discovery and Enhancement of Enzyme Function: A Review

Ibrahim

El‐Dewany

2018

Egypt. J. Microbiol.

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R ECENTLY, there is a growing recognition of enzymes utilization as ecofriendly alternative for chemical catalysts in various industrial sectors. Two main approaches are used to expand the enzymes utilization in various industrial applications including (i) Searching nature for discovery of better performing novel biocatalysts and (ii) Improvement of the enzyme function and properties. The recent advances in biocatalyst discovery and engineering have led to an increase of the efficiency and functional diversityof enzymes. Therefore, enzyme application is expanding to broader industrial processes, including those which were previously restricted to the classical chemical catalysts. Furthermore, there is a significant success in developing made-to-order enzymes that meet the industrial process conditions requirements, leading to more efficient and cost-effective processes. Here I highlight state-of-the-art strategies and tools for novel enzyme discovery and function enhancement, including enzyme engineering at the molecular level through different approaches such as directed evolution, rational design, semi rational design and computational de novo enzyme design; in addition to the recently developed field, nanobiocatalysis.

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Nanotechnology based activation-immobilization of psychrophilic pectate lyase: A novel approach towards enzyme stabilization and enhanced activity

Cited by 18 publications

References 38 publications

Inner-View of Nanomaterial Incited Protein Conformational Changes: Insights into Designable Interaction

Inner-View of Nanomaterial Incited Protein Conformational Changes: Insights into Designable Interaction

Use of Nanomaterials for the Immobilization of Industrially Important Enzymes

Recent Trends for Discovery and Enhancement of Enzyme Function: A Review

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