Nanotechnology‐Based Strategies for the Detection and Quantification of MicroRNA

Degliangeli, Federica; Pompa, Pier Paolo; Fiammengo, Roberto

doi:10.1002/chem.201402649

Cited by 58 publications

(30 citation statements)

References 147 publications

(119 reference statements)

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“…13,14 While Northern blotting is the only technique that allows for the quantitative visualization of miRNAs, it has low detection efficiency and requires complex methods that cantamination. 15 Microarrays, which allow simultaneous detection of several hundred miRNAs, are only semiquantitative making them most suitable for comparing relative expression levels of miRNA between different cellular states.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed Detection of MicroRNA Biomarkers Using SERS-Based Inverse Molecular Sentinel (iMS) Nanoprobes

et al. 2016

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MicroRNAs (miRNAs) have demonstrated great promise as a novel class of biomarkers for early detection of various cancers, including breast cancer. However, due to technical difficulties in detecting these small molecules, miRNAs have not been adopted into routine clinical practice for early diagnostics. Thus, it is important to develop alternative detection strategies that could offer more advantages over conventional methods. Here, we demonstrate the application of a “turn-on” SERS sensing technology, referred to as “inverse Molecular Sentinel (iMS)” nanoprobes, as a homogeneous assay for multiplexed detection of miRNAs. This SERS nanoprobe involves the use of plasmonic-active nanostars as the sensing platform. The “OFF-to-ON” signal switch is based on a nonenzymatic strand-displacement process and the conformational change of stem-loop (hairpin) oligonucleotide probes upon target binding. This technique was previously used to detect a synthetic DNA sequence of interest. In this study, we modified the design of the nanoprobe to be used for the detection of short (22-nt) miRNA sequences. The demonstration of using iMS nanoprobes to detect miRNAs in real biological samples was performed with total small RNA extracted from breast cancer cell lines. The multiplex capability of the iMS technique was demonstrated using a mixture of the two differently labeled nanoprobes to detect miR-21 and miR-34a miRNA biomarkers for breast cancer. The results of this study demonstrate the feasibility of applying the iMS technique for multiplexed detection of short miRNAs molecules.

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Section: Introductionmentioning

confidence: 99%

Multiplexed Detection of MicroRNA Biomarkers Using SERS-Based Inverse Molecular Sentinel (iMS) Nanoprobes

et al. 2016

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“…Thus, many recent attempts at platform development (both uniplex and multiplex) have capitalized on adapting high-sensitivity transducers to novel, selectivity-enhanced recognition elements. As many excellent reviews (25,(61)(62)(63)(64)(65) have provided in-depth background on the various biosensor platforms and techniques, we limit our discussion to recent examples of this trend that utilize direct (unlabeled) miR detection methods.…”

Section: Biosensor Techniquesmentioning

confidence: 98%

MicroRNA Detection: Current Technology and Research Strategies

Hunt

Broyles

Head

et al. 2015

Annual Rev. Anal. Chem.

161

130

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The relatively new field of microRNA (miR) has experienced rapid growth in methodology associated with its detection and bioanalysis as well as with its role in -omics research, clinical diagnostics, and new therapeutic strategies. The breadth of this area of research and the seemingly exponential increase in number of publications on the subject can present scientists new to the field with a daunting amount of information to evaluate. This review aims to provide a collective overview of miR detection methods by relating conventional, established techniques [such as quantitative reverse transcription polymerase chain reaction (RT-qPCR), microarray, and Northern blotting (NB)] and relatively recent advancements [such as next-generation sequencing (NGS), highly sensitive biosensors, and computational prediction of microRNA/targets] to common miR research strategies. This should guide interested readers toward a more focused study of miR research and the surrounding technology.

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“…Chemical and physical properties of nanostructures and nanomaterials have often been exploited to enhance the sensitivity for miRs’ detection [ 94 ]. In particular, efforts have been paid to developing different kinds of functional nanomaterials, such as noble metal nanoparticles, magnetic nanoparticles, quantum dots, carbon-based nanomaterials, with the aim to push the LOD further down to picomolar [ 95 ]. In this context, the optical detection of fluorescence signals produced by labelled probes is often exploited.…”

Section: Pna-based Biosensorsmentioning

confidence: 99%

Peptide Nucleic Acid-Based Biosensors for Cancer Diagnosis

2017

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The monitoring of DNA and RNA biomarkers freely circulating in the blood constitutes the basis of innovative cancer detection methods based on liquid biopsy. Such methods are expected to provide new opportunities for a better understanding of cancer disease at the molecular level, thus contributing to improved patient outcomes. Advanced biosensors can advance possibilities for cancer-related nucleic acid biomarkers detection. In this context, peptide nucleic acids (PNAs) play an important role in the fabrication of highly sensitive biosensors. This review provides an overview of recently described PNA-based biosensors for cancer biomarker detection. One of the most striking features of the described detection approaches is represented by the possibility to detect target nucleic acids at the ultra-low concentration with the capability to identify single-base mutations.

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Nanotechnology‐Based Strategies for the Detection and Quantification of MicroRNA

Cited by 58 publications

References 147 publications

Multiplexed Detection of MicroRNA Biomarkers Using SERS-Based Inverse Molecular Sentinel (iMS) Nanoprobes

Multiplexed Detection of MicroRNA Biomarkers Using SERS-Based Inverse Molecular Sentinel (iMS) Nanoprobes

MicroRNA Detection: Current Technology and Research Strategies

Peptide Nucleic Acid-Based Biosensors for Cancer Diagnosis

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