Nanotechnology Derived Nanotools in Biomedical Perspectives: An Update

Patil, Abhinandan; Mishra, Vijay; Thakur, Sourav; Riyaz, Bushra; Kaur, Amanjot; Khursheed, Rubiya; Patil, Kiran; Sathe, B. S.

doi:10.2174/1573413714666180426112851

Cited by 37 publications

(18 citation statements)

References 53 publications

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“…Nanomaterials continue to find applications in nanomedicine, such as in biosensors, medical devices and combination products, and as theranostic agents and drug delivery carriers due to their unique physicochemical and optical properties (Chen, Roy, Yang, & Prasad, ; Patil et al, ). Some of these properties, e.g., large surface area per mass volume, morphology, surface charge and high surface reactivity, also raise concerns about potential adverse human health effects and warrant additional safety evaluation.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity, cellular uptake and apoptotic responses in human coronary artery endothelial cells exposed to ultrasmall superparamagnetic iron oxide nanoparticles

Palacios-Hernández

Diaz-Diestra

Nguyen

et al. 2020

J of Applied Toxicology

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Ultrasmall superparamagnetic iron oxide nanoparticles (USPION) possess reactive surfaces, are metabolized and exhibit unique magnetic properties. These properties are desirable for designing novel theranostic biomedical products; however, toxicity mechanisms of USPION are not completely elucidated. The goal of this study was to investigate cell interactions (uptake and cytotoxicity) of USPION using human coronary artery endothelial cells as a vascular cell model. Polyvinylpirrolidone-coated USPION were characterized: average diameter 17 nm (transmission electron microscopy [TEM]), average hydrodynamic diameter 44 nm (dynamic light scattering) and zeta potential −38.75 mV. Cells were exposed to 0 (control), 25, 50, 100 or 200 μg/mL USPION. Concentration-and time-dependent cytotoxicity were observed after 3-6 hours through 24 hours of exposure using Alamar Blue and Real-Time Cell Electronic Sensing assays. Cell uptake was evaluated by imaging using live-dead confocal microscopy, actin and nuclear fluorescent staining, and TEM. Phase-contrast, confocal microscopy, and TEM imaging showed significant USPION internalization as early as 3 hours after exposure to 25 μg/mL. TEM imaging demonstrated particle internalization in secondary lysosomes with perinuclear localization. Three orthogonal assays were conducted to assess apoptosis. TUNEL staining demonstrated a marked increase in fragmented DNA, a response pathognomonic of apoptosis, after a 4hour exposure. Cells subjected to agarose gel electrophoresis exhibited degraded DNA 3 hours after exposure. Caspase-3/7 activity increased after a 3-hour exposure. USPION uptake resulted in cytotoxicity involving apoptosis and these results contribute to further mechanistic understanding of the USPION toxicity in vitro in cardiovascular endothelial cells. K E Y W O R D S apoptosis, cytotoxicity, overendocytosis, ultrasmall superparamagnetic iron oxide nanoparticles Abbreviation: USPION, ultrasmall superparamagnetic iron oxide nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity, cellular uptake and apoptotic responses in human coronary artery endothelial cells exposed to ultrasmall superparamagnetic iron oxide nanoparticles

Palacios-Hernández

Diaz-Diestra

Nguyen

et al. 2020

J of Applied Toxicology

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“…Gene delivery technologies have become an emerging approach in biomedical fields. 37,38 High transduction efficiency and low insertional mutagenesis have rendered adenoviral vectors as attractive gene delivery vehicles. 39 Moreover, adenoviral vectors exhibit strong and sufficient effects.…”

Section: Discussionmentioning

confidence: 99%

Human β-defensin 3 gene modification promotes the osteogenic differentiation of human periodontal ligament cells and bone repair in periodontitis

et al. 2020

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Efforts to control inflammation and achieve better tissue repair in the treatment of periodontitis have been ongoing for years. Human β-defensin 3, a broad-spectrum antimicrobial peptide has been proven to have a variety of biological functions in periodontitis; however, relatively few reports have addressed the effects of human periodontal ligament cells (hPDLCs) on osteogenic differentiation. In this study, we evaluated the osteogenic effects of hPDLCs with an adenoviral vector encoding human β-defensin 3 in an inflammatory microenvironment. Then human β-defensin 3 gene-modified rat periodontal ligament cells were transplanted into rats with experimental periodontitis to observe their effects on periodontal bone repair. We found that the human β-defensin 3 gene-modified hPDLCs presented with high levels of osteogenesis-related gene expression and calcium deposition. Furthermore, the p38 MAPK pathway was activated in this process. In vivo, human β-defensin 3 gene-transfected rat PDLCs promoted bone repair in SD rats with periodontitis, and the p38 mitogen-activated protein kinase (MAPK) pathway might also have been involved. These findings demonstrate that human β-defensin 3 accelerates osteogenesis and that human β-defensin 3 gene modification may offer a potential approach to promote bone repair in patients with periodontitis.

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“…For biomedical applications, the main challenge is how to synthesis nanoparticles by controlling their physicochemical properties like size, charge, and polydispersity ( 9 10 ). Thus, the nanoparticle fabrication method should be reproducible and reliable.…”

Section: Introductionmentioning

confidence: 99%

Chitosan/tripolyphosphate nanoparticles in active and passive microchannels

Akbari

Rahimi

2021

Research in Pharmaceutical Sciences

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Background and purpose: In recent years, the interest in chitosan nanoparticles has increased due to their application, especially in drug delivery. The main aim of this work was to find a suitable method for simulating pharmaceutical nanoparticles with computational fluid dynamics (CFD) modeling and use it for understanding the process of nanoparticle formation in different types of microchannels. Experimental approach: Active and passive microchannels were compared to find the advantages and disadvantages of each system. Twenty-eight experiments were done on microchannels to quantify the effect of 4 parameters and their interactions on the size and polydispersity index (PDI) of nanoparticles. CFD was implemented by coupling reactive kinetics and the population balance method to simulate the synthesis of chitosan/tripolyphosphate nanoparticles in the microchannel. Findings/Results: The passive microchannel had the best performance for nanoparticle production. The most uniform microspheres and the narrowest standard deviation (124.3 nm, PDI = 0.112) were achieved using passive microchannel. Compared to the active microchannel, the size and PDI of the nanoparticles were 28.7% and 70.5% higher for active microchannels, and 55.43% and 105.3% higher for simple microchannels, respectively. Experimental results confirmed the validity of CFD modeling. The growth and nucleation rates were determined using the reaction equation of chitosan and tripolyphosphate. Conclusion and implications: CFD modeling by the proposed method can play an important role in the prediction of the size and PDI of chitosan/tripolyphosphate nanoparticles in the same condition and provide a new perspective for studying the production of nanoparticles by numerical methods.

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Nanotechnology Derived Nanotools in Biomedical Perspectives: An Update

Cited by 37 publications

References 53 publications

Cytotoxicity, cellular uptake and apoptotic responses in human coronary artery endothelial cells exposed to ultrasmall superparamagnetic iron oxide nanoparticles

Cytotoxicity, cellular uptake and apoptotic responses in human coronary artery endothelial cells exposed to ultrasmall superparamagnetic iron oxide nanoparticles

Human β-defensin 3 gene modification promotes the osteogenic differentiation of human periodontal ligament cells and bone repair in periodontitis

Chitosan/tripolyphosphate nanoparticles in active and passive microchannels

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