Nanotechnology Development and Utilization: A Primer for Diagnostic and Interventional Radiologists

Monsky, Wayne L.; Vien, Derek S.; Link, Daniel P.

doi:10.1148/rg.315105238

Cited by 13 publications

(10 citation statements)

References 68 publications

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“…Nanoparticles (NPs) have been proposed as promising tools for therapy, drug delivery, imaging, and active pharmaceutical ingredients [1][2][3][4][5]. An early and crucial stage in drug development is toxicity testing.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Bancos

Tsai

Hackley

et al. 2012

ISRN Nanotechnology

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Nanoparticles (NPs) are known to interfere with many high-throughput cell viability and cell proliferation assays, which complicates the assessment of their potential toxic effects. The aim of this study was to compare viability and proliferation results for colloidal silica (SiO 2 NP; 7 nm) in the RAW 264.7 mouse macrophage cell line using three different techniques: plate-based assays, flow cytometry analysis, and Coulter counter assays. Our data indicate that CellTiter-Blue, XTT, and CyQuant plate-based assays show increased values over control at low SiO 2 NPs concentrations (0.001-0.01 g/L). SiO 2 NPs show little-to-no interference with flow cytometry and Coulter counter assays, which not only were more reliable in determining cell viability and proliferation at low concentrations in vitro, but also identified changes in cell granularity and size that were not captured by the plate-based assays. At high SiO 2 NP concentrations (1 g/L) all techniques indicated cytotoxicity. In conclusion, flow cytometry and Coulter counter identified new cellular features, and flow cytometry offered more flexibility in analyzing the viability and proliferation profile of SiO 2 NP-treated RAW 264.7 cells.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Bancos

Tsai

Hackley

et al. 2012

ISRN Nanotechnology

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“…10,31 Assessment of the distribution of our gene vector in vivo indicates that a percutaneous intratumoral delivery strategy can successfully deliver the loaded gene (pLuci), while limiting its expression to the target tumor tissue ( Figure 3C). In contrast, conventional intravenous delivery of most nanoparticle platforms results in sequestration of nanoparticles in the organs of the reticuloendothelial system, leading to subtherapeutic dosing.…”

Section: Resultsmentioning

confidence: 99%

“…8,9 While adapting the therapeutic techniques of interventional radiology, MRI-guided nanoparticle delivery could enhance uptake of an intratumoral agent and limit its nonspecific delivery to the reticuloendothelial system. 10,11 In support of this concept, some studies have reported on application of MRI-guided intra-arterial 12 and intrabiliary 13 nanoparticles for the treatment of liver tumors. Cell-penetrating peptides (CPPs) are short 30-residue synthetic peptides and represent one of the most promising strategies for enhancing the permeability of therapeutic agents, despite the limitations of various biomolecules with regard to extracellular and intracellular diffusion.…”

mentioning

confidence: 94%

Magnetic resonance-guided regional gene delivery strategy using a tumor stroma-permeable nanocarrier for pancreatic cancer

Wang¹,

Li²,

An³

et al. 2015

IJN

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Background Gene therapy is a very promising technology for treatment of pancreatic ductal adenocarcinoma (PDAC). However, its application has been limited by the abundant stromal response in the tumor microenvironment. The aim of this study was to prepare a dendrimer-based gene-free loading vector with high permeability in the tumor stroma and explore an imaging-guided local gene delivery strategy for PDAC to promote the efficiency of targeted gene delivery. Methods The experimental protocol was approved by the animal ethics committee of Zhongshan Hospital, Fudan University. Third-generation dendrigraft poly-L-lysines was selected as the nanocarrier scaffold, which was modified by cell-penetrating peptides and gadolinium (Gd) chelates. DNA plasmids were loaded with these nanocarriers via electrostatic interaction. The cellular uptake and loaded gene expression were examined in MIA PaCa-2 cell lines in vitro. Permeability of the nanoparticles in the tumor stroma and transfected gene distribution in vivo were studied using a magnetic resonance imaging-guided delivery strategy in an orthotopic nude mouse model of PDAC. Results The nanocarriers were synthesized with a dendrigraft poly-L-lysine to polyethylene glycol to DTPA ratio of 1:3.4:8.3 and a mean diameter of 110.9±7.7 nm. The luciferases were strictly expressed in the tumor, and the luminescence intensity in mice treated by Gd-DPT/plasmid luciferase (1.04×10 4 ±9.75×10 2 p/s/cm 2 /sr) was significantly ( P <0.05) higher than in those treated with Gd-DTPA (9.56×10 2 ±6.15×10 p/s/cm 2 /sr) and Gd-DP (5.75×10 3 ± 7.45×10 2 p/s/cm 2 /sr). Permeability of the nanoparticles modified by cell-penetrating peptides was superior to that of the unmodified counterpart, demonstrating the improved capability of nanoparticles for diffusion in tumor stroma on magnetic resonance imaging. Conclusion This study demonstrated that an image-guided gene delivery system with a stroma-permeable gene vector could be a potential clinically translatable gene therapy strategy for PDAC.

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“…Miniaturisation and nanotechnology may revolutionise medical practice (Emerich and Thanos 2003, Freitas 2005, Yang 2007, Monsky et al 2011, including in resource-poor environments , Mehta 2008). For example, small and simple imaging instruments have the potential to increase greatly access to obstetrical monitoring, thereby reducing maternal mortality and poor pregnancy outcomes, as well as absorbing some health-worker shortages.…”

Section: What Is In the Pipeline?mentioning

confidence: 99%

Innovation and technology for global public health

Piot

2012

Global Public Health

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Recent decades have been marked by the explosive development of innovative scientific, technological and business products and processes. Despite their immense impact on health globally, little has been accomplished in the field of global public health to incorporate, address and harness such innovations in practice. In order to meet the world's growing health needs, it is essential that global public health accepts and adapts to these innovations. Moreover, such innovations must be implemented equitably in ways that will best serve their intended recipients, without deepening health- and access-related disparities. This article will briefly discuss the wide array of technologies in the pipeline that will affect global public health practice, their impact on the field and on populations and the issues facing the field in adopting these innovations.

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Nanotechnology Development and Utilization: A Primer for Diagnostic and Interventional Radiologists

Cited by 13 publications

References 68 publications

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Magnetic resonance-guided regional gene delivery strategy using a tumor stroma-permeable nanocarrier for pancreatic cancer

Innovation and technology for global public health

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