Nanotechnology for Detection of Small Mass Difference

Gupta, S. V.

doi:10.1007/978-3-642-23412-5_11

“…Silicon micro-cantilever which plays the role of the sensing element is mounted on the positioning system. In experiments, we used two cantilevers: cantilever A with dimensions 233×45×4 µm 3 and cantilever B with dimensions 215×40×15 µm 3 . Laser light pulses at wavelength 532 nm each of energy 0.5 mJ and duration 7 ns were used to excite the cantilever out-of-plane mechanical vibrations.…”

Section: Methodsmentioning

confidence: 99%

“…is to be determined. Alternative approach for mass measurement is based on using the micromechanical resonator as an inertial balance where oscillation frequency is shifted by small quantities of adsorbed mass [1][2][3][4][5]. In such applications, an interferometer is considered as a promising tool for detecting the resonator oscillations which are of ultra-small amplitude [1].…”

Section: Introductionmentioning

confidence: 99%

Resonance Micro-Weighing of Sub-Picogram Mass with the Use of Adaptive Interferometer

Romashko

¹

,

Efimov

²

,

Kulchin

³

2014

Measurement Science Review

5

0

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Mass of macroscopic object is easily measured by a suitable balance. However, this approach becomes inapplicable if mass of microscopic object is to be determined. Alternative approach for mass measurement is based on using the micromechanical resonator as an inertial balance where oscillation frequency is shifted by small quantities of adsorbed mass. In this work we present experimental results of applying an adaptive interferometry technique based on dynamic hologram recorded in photorefractive CdTe crystal for measuring picogram mass adsorbed on micromechanical resonators with dimensions 215×40×15 µm 3 . It is also shown that the resonance micro-weighing system based on adaptive interferometer has potential for reducing the threshold of mass detection down to 10 -17 g in the case of using a resonator with sub-micron dimensions.

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“…Most existing tools rely on volumetric measurements of cells, as the volume is an indirect measure of biomass, with inaccuracies arising from osmotic changes and other processes in single cells (3,13,14). Cell masses range from several nanograms to a few picograms , for example, a yeast cell mass was measured at 11 pg (15). In addition, mass changes due to cellular processes can occur within milliseconds (16) making both mass and temporal resolution essential.…”

Section: Introductionmentioning

confidence: 99%

AFM microfluidic cantilevers as weight sensors for live single cell mass measurements

Chien

¹

,

Jiang

²

,

Gong

³

et al. 2022

Meas. Sci. Technol.

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The inability to reliably measure small mass changes at the single-cell level has been a challenge in biological research. In this manuscript, we report the use of microfluidic cantilevers in liquid with picogram-scale weight sensing capability for the measurement of cellular mass changes of living single cells. With this instrumentation, we were able to perform fast mass measurements within 3 minutes. We show results of mass measurements of microspheres with errors within 100 picograms as well as single-cell mass measurements in a physiologically relevant environment. We also performed finite element analysis to simulate and optimize the structural design and materials of cantilevers. Our simulation results indicate that using polymer materials, such as SU8 and polyimide, could improve the minimal detectable mass by 3-fold compared to conventional silicon cantilevers. The simulations also suggest that smaller dimensions of length, width, and thickness would improve the mass detection capability of microfluidic cantilevers.

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“…(4)(5)(6)(7)(8)(9)(10)(11)(12) Most existing tools rely on volumetric measurements of cells, as the volume is an indirect measure of biomass, and are susceptible to osmotic changes and other processes in single cells. (3,13,14) Cell masses range from a few picograms to a few nanograms (15) and mass changes due to cellular processes could happen within millisecond timeframe (16), making mass and temporal resolution essential.…”

Section: Introductionmentioning

confidence: 99%

AFM Microfluidic Cantilevers as Weight Sensors for Live Single Cell Mass Measurements

Chien

¹

,

Jiang

²

,

Li

³

et al. 2022

Preprint

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The inability to reliably measure small mass changes at the single-cell level has been a challenge in biological research. In this manuscript, we report the use of microfluidic cantilevers in liquid with picogram-scale weight sensing capability for the measurement of cellular mass changes of living single cells. With this instrumentation, we were able to perform fast mass measurements within 3 minutes. We show results of mass measurements of microspheres with errors within 100 picograms as well as single-cell mass measurements in a physiologically relevant environment. We also performed finite element analysis to simulate and optimize the structural design and materials of cantilevers. Our simulation results indicate that using polymer materials, such as SU8 and polyimide, could improve the minimal detectable mass by 3-fold compared to conventional silicon cantilevers. The simulations also suggest that smaller dimensions of length, width, and thickness would improve the mass detection capability of microfluidic cantilevers.

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Nanotechnology for Detection of Small Mass Difference

Cited by 4 publications

References 93 publications

Resonance Micro-Weighing of Sub-Picogram Mass with the Use of Adaptive Interferometer

Resonance Micro-Weighing of Sub-Picogram Mass with the Use of Adaptive Interferometer

AFM microfluidic cantilevers as weight sensors for live single cell mass measurements

AFM Microfluidic Cantilevers as Weight Sensors for Live Single Cell Mass Measurements

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