Nanotechnology in reproductive medicine: Emerging applications of nanomaterials

Barkalina, Natalia; Charalambous, Charis; Jones, Céline; Coward, Kevin

doi:10.1016/j.nano.2014.01.001

Cited by 160 publications

(80 citation statements)

References 163 publications

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“…Collagen coated culture flasks were used when necessary and water was always used in demineralised form, HAuCl 4 was purchased from Himedia. The compounds were dissolved at 10 mM in ddH 2 O and DMSO as a stock solution, and then further diluted to desired concentrations for in vitro experiments. Cell Titer 96A Queous One Solution Cell proliferation Assay, Promega, 2'7'-dichlorofluo-rescein diacetate (H 2 DCFDA), Tetra-methyl-rhodamine (TMRE) (Invitrogen, Carlsbad, CA) were purchased from Biogenix, India.…”

Section: Methodsmentioning

confidence: 99%

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

Parida¹,

Biswal²,

Bindhani³

2014

ABC

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In this investigation, the anticancer potentiality and biological mechanism of gold nanoparticles (AuNPs) was studied in SUDHL-4 cell line. Metallic AuNPs were prepared and stabilized with ethanol clove (Syzygium aromaticum) extract. The green synthesis of AuNPs was characterized and evaluated by UV-Visible Spectroscopic, X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and biological activities using various biochemical assays. Green synthesis of AuNPs was confirmed by instrument method. The TEM images show polydisperse, mostly spherical AuNPs particles of 12 -20 nm. AuNPs were decreased the growth and viability of SU-DHL-4 cell line and increased the apoptosis. The treatments of SU-DHL-4 cells with AuNPs resulted in a moderate considerably increase in Reactive oxygen species (ROS) production. We measured apoptosis by Annexin-V/propidium iodide (PI) in the existence and nonexistence of the antioxidant N-acetyl-Lcysteine (NAC), the glutathione-depleting agent buthionine sulfoximine (BSO), or caspase inhibitors to determine the mechanism of cell death. AuNPs are unique potential anticancer agents that cause ROS-dependent apoptosis in SUDHL-4 cell line which was improved by depletion of glutathione (GHS) and inhibited by N-acetyl-L-cysteine on Z-VAD-FMK.

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Section: Methodsmentioning

confidence: 99%

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

Parida¹,

Biswal²,

Bindhani³

2014

ABC

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“…Organic nanomaterials are composed mainly of carbon and polymers such as chitosan (Figure 1). Inorganic ones consist of noble metals, oxides and semiconductors with optical properties, such as cadmium, selenium, tellurium (Barkalina et al, 2014a).…”

Section: Nanotechnology -What Is It?mentioning

confidence: 99%

“…Materials produced through nanotechnology or by classical technology, but having a size smaller than 100 nm, are used on a very large scale in practical applications, including energy supply, construction and transportation, food production, data storage and telecommunications, healthcare, the manufacturing of consumer products and biotechnology (Barkalina et al, 2014a).…”

Section: Nanotechnology -What Is It?mentioning

confidence: 99%

Nanobiotechnology in reproduction – pros and cons. A review

Zielińska‐Górska

Górski

2015

J. Anim. Feed Sci.

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“…Therefore, short-, medium-, and long-term effects of these nanomaterials are yet to be understood completely. 138 Ranging from the combination of materials or devices with drugs and biomolecules, adding benefits like slow and controlled drug release, promoting greater efficiency of tissue penetration to greater protection against drug degradation, nanotechnology can be used to prevent, monitor, control, and cure diseases in several ways. 137 At present, the main types of nanosystems used for the delivery of bioactive substances are liposomes (LIPs), microemulsions (MEs), nanoemulsions, cyclodextrins (CDs), solid lipid nanoparticles (SLNs), polymeric nanoparticles (PNs), and metallic nanoparticles (MNPs).…”

mentioning

confidence: 99%

Nanotechnology-based drug delivery systems for control of microbial biofilms: a review

Ramos¹,

Silva²,

Spósito³

et al. 2018

IJN

220

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Since the dawn of civilization, it has been understood that pathogenic microorganisms cause infectious conditions in humans, which at times, may prove fatal. Among the different virulent properties of microorganisms is their ability to form biofilms, which has been directly related to the development of chronic infections with increased disease severity. A problem in the elimination of such complex structures (biofilms) is resistance to the drugs that are currently used in clinical practice, and therefore, it becomes imperative to search for new compounds that have anti-biofilm activity. In this context, nanotechnology provides secure platforms for targeted delivery of drugs to treat numerous microbial infections that are caused by biofilms. Among the many applications of such nanotechnology-based drug delivery systems is their ability to enhance the bioactive potential of therapeutic agents. The present study reports the use of important nanoparticles, such as liposomes, microemulsions, cyclodextrins, solid lipid nanoparticles, polymeric nanoparticles, and metallic nanoparticles, in controlling microbial biofilms by targeted drug delivery. Such utilization of these nanosystems has led to a better understanding of their applications and their role in combating biofilms.

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Nanotechnology in reproductive medicine: Emerging applications of nanomaterials

Cited by 160 publications

References 163 publications

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

Nanobiotechnology in reproduction – pros and cons. A review

Nanotechnology-based drug delivery systems for control of microbial biofilms: a review

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