Nanotechnology-leveraged nucleic acid amplification for foodborne pathogen detection

Deng, Ruijie; Bai, Jinrong; Yang, Hao; Ren, Yao; He, Qiang; Lu, Yunhao

doi:10.1016/j.ccr.2024.215745

Search citation statements

Order By: Relevance

Paper Sections

Select...

Detection Of Viral Nucleic Acids1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

2025

Publication Types

Select...

Article6

Relationship

Self Cite0

Independent6

Authors

Journals

Cited by 6 publications

(1 citation statement)

References 154 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanomaterials have played a huge role in the development of biosensors. 51–53 Jeon et al described a sample preparation chip embedded with a bi-porous silica nanomembrane for pathogen and nucleic acid enrichment/separation. The chip has a unique bi-porous nanostructure consisting of a macroporous layer and a small porous layer.…”

Section: Detection Of Viral Nucleic Acidsmentioning

confidence: 99%

Advances in rapid point-of-care virus testing

Zhang,

Bu,

Shu

et al. 2024

Analyst

View full text Add to dashboard Cite

show abstract

Section: Detection Of Viral Nucleic Acidsmentioning

confidence: 99%

Advances in rapid point-of-care virus testing

Zhang,

Bu,

Shu

et al. 2024

Analyst

View full text Add to dashboard Cite

show abstract

Unsupervised Learning‐Assisted Acoustic‐Driven Nano‐Lens Holography for the Ultrasensitive and Amplification‐Free Detection of Viable Bacteria

Zhou,

Zhao,

Wen

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Bacterial infection is a crucial factor resulting in public health issues worldwide, often triggering epidemics and even fatalities. The accurate, rapid, and convenient detection of viable bacteria is an effective method for reducing infections and illness outbreaks. Here, an unsupervised learning–assisted and surface acoustic wave–interdigital transducer‐driven nano‐lens holography biosensing platform is developed for the ultrasensitive and amplification‐free detection of viable bacteria. The monitoring device integrated with the nano‐lens effect can achieve the holographic imaging of polystyrene microsphere probes in an ultra‐wide field of view (∽28.28 mm2), with a sensitivity limit of as low as 99 nm. A lightweight unsupervised learning hologram processing algorithm considerably reduces training time and computing hardware requirements, without requiring datasets with manual labels. By combining phage–mediated viable bacterial DNA extraction and enhanced CRISPR–Cas12a systems, this strategy successfully achieves the ultrasensitive detection of viable Salmonella in various real samples, demonstrating enhanced accuracy validated with the qPCR benchmark method. This approach has a low cost (∽$500) and is rapid (∽1 h) and highly sensitive (∽38 CFU mL−1), allowing for the amplification‐free detection of viable bacteria and emerging as a powerful tool for food safety inspection and clinical diagnosis.

show abstract

Coordination chemistry in CRISPR-Cas-based point of care testing: A review of molecular probe development and applications

Jia,

Khan,

et al. 2024

Coordination Chemistry Reviews

View full text Add to dashboard Cite

Nanotechnology-leveraged nucleic acid amplification for foodborne pathogen detection

Cited by 6 publications

References 154 publications

Advances in rapid point-of-care virus testing

Advances in rapid point-of-care virus testing

Unsupervised Learning‐Assisted Acoustic‐Driven Nano‐Lens Holography for the Ultrasensitive and Amplification‐Free Detection of Viable Bacteria

Coordination chemistry in CRISPR-Cas-based point of care testing: A review of molecular probe development and applications

Contact Info

Product

Resources

About