Nanotechnology to advance CRISPR–Cas genetic engineering of plants

Demirer, Gözde S.; Silva, Tallyta Nayara; Jackson, Christopher T.; Thomas, Jason B.; Ehrhardt, David; Rhee, Seung Y.; Mortimer, Jenny C.; Landry, Markita P.

doi:10.1038/s41565-021-00854-y

Cited by 164 publications

(93 citation statements)

References 57 publications

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“…Synthetic genomics approaches will be bolstered by nanoparticle gene delivery due to the ability to tune characteristics of the delivery chassis, deliver a wider array of genes for more applications at once, and allow gene delivery to precise organelles. In addition, nanotechnology approaches may be employed for CRISPR-Cas genetic engineering of plants ( Demirer et al, 2021 ). With the chloroplast’s DNA repair mechanism nearly exclusively homology-driven, current approaches for plastid genetic engineering rely on delivering antibiotic markers with homologous arms for integration.…”

Section: Future Research In Chloroplast Nanobiotechnologymentioning

confidence: 99%

Nanotechnology Approaches for Chloroplast Biotechnology Advancements

Newkirk

Allende²,

Jinkerson³

et al. 2021

Front. Plant Sci.

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Photosynthetic organisms are sources of sustainable foods, renewable biofuels, novel biopharmaceuticals, and next-generation biomaterials essential for modern society. Efforts to improve the yield, variety, and sustainability of products dependent on chloroplasts are limited by the need for biotechnological approaches for high-throughput chloroplast transformation, monitoring chloroplast function, and engineering photosynthesis across diverse plant species. The use of nanotechnology has emerged as a novel approach to overcome some of these limitations. Nanotechnology is enabling advances in the targeted delivery of chemicals and genetic elements to chloroplasts, nanosensors for chloroplast biomolecules, and nanotherapeutics for enhancing chloroplast performance. Nanotechnology-mediated delivery of DNA to the chloroplast has the potential to revolutionize chloroplast synthetic biology by allowing transgenes, or even synthesized DNA libraries, to be delivered to a variety of photosynthetic species. Crop yield improvements could be enabled by nanomaterials that enhance photosynthesis, increase tolerance to stresses, and act as nanosensors for biomolecules associated with chloroplast function. Engineering isolated chloroplasts through nanotechnology and synthetic biology approaches are leading to a new generation of plant-based biomaterials able to self-repair using abundant CO2 and water sources and are powered by renewable sunlight energy. Current knowledge gaps of nanotechnology-enabled approaches for chloroplast biotechnology include precise mechanisms for entry into plant cells and organelles, limited understanding about nanoparticle-based chloroplast transformations, and the translation of lab-based nanotechnology tools to the agricultural field with crop plants. Future research in chloroplast biotechnology mediated by the merging of synthetic biology and nanotechnology approaches can yield tools for precise control and monitoring of chloroplast function in vivo and ex vivo across diverse plant species, allowing increased plant productivity and turning plants into widely available sustainable technologies.

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Section: Future Research In Chloroplast Nanobiotechnologymentioning

confidence: 99%

Nanotechnology Approaches for Chloroplast Biotechnology Advancements

Newkirk

Allende²,

Jinkerson³

et al. 2021

Front. Plant Sci.

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“…71 The complex structure of the plant cell wall places strict requirements for the selection of penetrating nanocarriers in terms of their size, morphology, and surface properties. 72 Recently, Wang et al discussed the plant cell wall barrier, cellular entry, endosomal escape, and potential nanoparticles for inplanta protein delivery. 73 Alternatively, delivery strategy that only the cargo enters the cell could be considered to bypass the restriction from the plant cell wall.…”

Section: Nanotechnology In Next Generation Agrochemicalsmentioning

confidence: 99%

Eco‐friendly biomolecule‐nanomaterial hybrids as next‐generation agrochemicals for topical delivery

Huang

et al. 2021

EcoMat

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The agrochemical is often applied to enhance the agricultural production.Nanotechnology has advanced agricultural biotechnology in the new "agritech revolution." Eco-friendly nanoparticles as carriers can essentially actualize the application of biomolecules as sustainable agrochemicals to increase their biological performance and reduce deterioration of the ecosystem. Particularly, these nanocarrier-biomolecule hybrids will be ideal for topical applications to enhance the crop yield and quality. This review summarizes the current efforts in developing nanoparticles as carriers of biomolecules for topical delivery. First, the applicable biomolecules for plant growth regulation and the pathogen control are briefly introduced. The criteria and strategies for research and industrial development of nanocarrier-biomolecule hybrids are then proposed, which better involves both researchers and manufacturers in the beginning for an efficient R&D process. The review further discusses several typical nanoparticle-biomolecule hybrids as the candidates of nextgeneration agrochemicals with advanced scientific merits and high perspectives of industrialization as the conclusion.

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“…Here we have highlighted just a few exciting technological advances in delivering CRISPR gene editing to crop plants. Other emerging methods include nanotechnology-based delivery of CRISPR reagents, transient expression of transgenes by spraying viral or Agrobacterium nanoparticles, and machine learning algorithms for improving stable transformation [ 116 , 117 , 118 , 119 , 120 , 121 ]. Combining several of these methods will likely allow for increased editing and introgression of CRISPR alleles into cereal crops, including agriculturally important germplasm.…”

Section: Challenges and Emerging Technologies For Crispr/cas9-based Crop Improvementmentioning

confidence: 99%

Next Generation Cereal Crop Yield Enhancement: From Knowledge of Inflorescence Development to Practical Engineering by Genome Editing

Liu

Lindsay

Jackson

2021

IJMS

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Artificial domestication and improvement of the majority of crops began approximately 10,000 years ago, in different parts of the world, to achieve high productivity, good quality, and widespread adaptability. It was initiated from a phenotype-based selection by local farmers and developed to current biotechnology-based breeding to feed over 7 billion people. For most cereal crops, yield relates to grain production, which could be enhanced by increasing grain number and weight. Grain number is typically determined during inflorescence development. Many mutants and genes for inflorescence development have already been characterized in cereal crops. Therefore, optimization of such genes could fine-tune yield-related traits, such as grain number. With the rapidly advancing genome-editing technologies and understanding of yield-related traits, knowledge-driven breeding by design is becoming a reality. This review introduces knowledge about inflorescence yield-related traits in cereal crops, focusing on rice, maize, and wheat. Next, emerging genome-editing technologies and recent studies that apply this technology to engineer crop yield improvement by targeting inflorescence development are reviewed. These approaches promise to usher in a new era of breeding practice.

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Nanotechnology to advance CRISPR–Cas genetic engineering of plants

Cited by 164 publications

References 57 publications

Nanotechnology Approaches for Chloroplast Biotechnology Advancements

Nanotechnology Approaches for Chloroplast Biotechnology Advancements

Eco‐friendly biomolecule‐nanomaterial hybrids as next‐generation agrochemicals for topical delivery

Next Generation Cereal Crop Yield Enhancement: From Knowledge of Inflorescence Development to Practical Engineering by Genome Editing

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