Nanotextured titanium surfaces stimulate spreading, migration, and growth of rat mast cells

Abstract

Titanium is a biomaterial widely used in dental and orthopedic implants. Since tissue-implant interactions occur at the nanoscale level, nanotextured titanium surfaces may affect cellular activity and modulate the tissue response that occurs at the tissue-implant interface. Therefore, the characterization of diverse cell types in response to titanium surfaces with nanotopography is important for the rational design of implants. Mast cells are multifunctional cells of the immune system that release a range of chemical mediators involved in the inflammatory response that occurs at the tissue-implant interface. Therefore, the aim of this study was to investigate the effects of the nanotopography of titanium surfaces on the physiology of mast cells. The results show that the nanotopography of titanium surfaces promoted the spreading of mast cells, which was accompanied by the reorganization of the cytoskeleton. Also, the nanotopography of titanium surfaces enhanced cell migration and cell growth, but did not alter the number of adherent cells in first hours of culture or affect focal adhesions and mediator release. Thus, the results show that nanotopography of titanium surfaces can affect mast cell physiology, and represents an improved strategy for the rational production of surfaces that stimulate tissue integration with the titanium implants. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2150-2161, 2017.

Role of Hippo-YAP Signaling in Osseointegration by Regulating Osteogenesis, Angiogenesis, and Osteoimmunology

“…The classic M1/M2 dichotomy is still popular in the latest study (Gao et al, 2020; Figure 2). Moreover, besides macrophages, there are other immune cells being discussed to play their respective promising roles in osteoimmunologymediated osseointegration as well, such as T lymphocytes (Singhatanadgit et al, 2019) and mast cells (Zizzi et al, 2011;Marcatti Amarú Maximiano et al, 2017).…”

Nanoscale hybrid implant surfaces and Osterix‐mediated osseointegration

“…A reservoir of skeletal stem cells/progenitors, immune cells and relatively undifferentiated osteoprogenitor cells (mesenchymal stem cells) that direct osteogenesis are present in this location. 15,16 In cell culture, 8 in rodent models 17,18 and in humans, 19,20 implant topography alters implant adherent cell osteoinductive gene expression at the level of Runx2 and Osterix. The greater fold induction for Osterix compared to Runx2 expression in cells adherent to hybrid nano/micro versus micro topography surfaces implicates nanoscale features of the surface in signaling of osseointegration through and Osterix-dependent pathway.…”

Nature-Inspired Nanoflower Structures on Titanium Surface via Alkali Treatment for Biomedical Applications

“…The biocompatibility of titanium surfaces can be improved by the development of oxide layers with specific morphologies on the surface thereby improving the physico-chemical aspects of the surface. Nanostructured oxide films developed on titanium implant surface represents an efficient method to modulate the cell response for promoting bone growth, thereby improving the osseointegration [9][10][11][12][13]. Alkali-treatment on titanium surface is reported to generate nanostructured surface thereby improving the biocompatibility.…”