Nanotheranostic Interface Based on Antibiotic‐Loaded Conducting Polymer Nanoparticles for Real‐Time Monitoring of Bacterial Growth Inhibition

Enshaei, Hamidreza; Puiggalı́-Jou, Anna; Valle, Luís J. del; Turón, Pau; Saperas, Núria; Alemán, Carlos

doi:10.1002/adhm.202001636

Cited by 16 publications

(15 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More specifically, our strategy consists on the functionalization of surgical meshes with a sensor that was specifically developed to detect bacteria without interference of normal eukaryotic cells. 31,32 The sensor is based on the electrochemical detection of the oxidation of nicotinamide adenine dinucleotide (NADH) into NAD+. Although NADH is involved in the respiration reactions of both bacteria and normal eukaryotic cells, a distinctive feature allows the identification of bacterial colonization without interference from signals coming from normal cells.…”

Section: ■ Introductionmentioning

confidence: 99%

Preparation and Characterization of Functionalized Surgical Meshes for Early Detection of Bacterial Infections

Fontana‐Escartín

Hauadi

Lanzalaco

et al. 2023

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

Isotactic polypropylene (i-PP) nonabsorbable surgical meshes are modified by incorporating a conducting polymer (CP) layer to detect the adhesion and growth of bacteria by sensing the oxidation of nicotinamide adenine dinucleotide (NADH), a metabolite produced by the respiration reactions of such microorganisms, to NAD+. A three-step process is used for such incorporation: (1) treat pristine meshes with low-pressure O 2 plasma; (2) functionalize the surface with CP nanoparticles; and(3) coat with a homogeneous layer of electropolymerized CP using the nanoparticles introduced in (2) as polymerization nuclei. The modified meshes are stable and easy to handle and also show good electrochemical response. The detection by cyclic voltammetry of NADH within the interval of concentrations reported for bacterial cultures is demonstrated for the two modified meshes. Furthermore, Staphylococcus aureus and both biofilm-positive (B+) and biofilm-negative (B-) Escherichia coli cultures are used to prove real-time monitoring of NADH coming from aerobic respiration reactions. The proposed strategy, which offers a simple and innovative process for incorporating a sensor for the electrochemical detection of bacteria metabolism to currently existing surgical meshes, holds considerable promise for the future development of a new generation of smart biomedical devices to fight against post-operative bacterial infections.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Preparation and Characterization of Functionalized Surgical Meshes for Early Detection of Bacterial Infections

Fontana‐Escartín

Hauadi

Lanzalaco

et al. 2023

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Poly(3,4-ethylenedioxythiophene) (PEDOT, 13 ) is a type of PTA with excellent PTCE and good biocompatibility, so PEDOT-based OPAMs have been explored for antibacterial PTT. Meanwhile, it can absorb NIR light (700–1250 nm). − Li et al synthesized PEDOT nanoparticles via a hydrothermal strategy and then modified them with functional ligands, including PEG, ICG, GTA, and PSS. Upon NIR I irradiation, the ICG on the surface of PEDOT resulted in ROS-mediated PDT, while in the NIR II region it exhibited strong absorbance and presented photothermal ability for antibacterial PTT …”

Section: Basic Principles Of Organic Photo-antimicrobials For Phototh...mentioning

confidence: 99%

Organic Photo-antimicrobials: Principles, Molecule Design, and Applications

et al. 2021

View full text Add to dashboard Cite

The emergence of multi-drug-resistant pathogens threatens the healthcare systems world-wide. Recent advances in phototherapy (PT) approaches mediated by photo-antimicrobials (PAMs) provide new opportunities for the current serious antibiotic resistance. During the PT treatment, reactive oxygen species or heat produced by PAMs would react with the cell membrane, consequently leaking cytoplasm components and effectively eradicating different pathogens like bacteria, fungi, viruses, and even parasites. This Perspective will concentrate on the development of different organic photo-antimicrobials (OPAMs) and their application as practical therapeutic agents into therapy for local infections, wound dressings, and removal of biofilms from medical devices. We also discuss how to design highly efficient OPAMs by modifying the chemical structure or conjugating with a targeting component. Moreover, this Perspective provides a discussion of the general challenges and direction for OPAMs and what further needs to be done. It is hoped that through this overview, OPAMs can prosper and will be more widely used for microbial infections in the future, especially at a time when the global COVID-19 epidemic is getting more serious.

show abstract

“…The latter is of great importance not only for understanding and studying bacterial systems but also for developing energy generation/storage devices known as microbial fuel cells. As such, CPs have found widespread applications in antimicrobial surfaces, antibiotic research, , biosensing, , and biofuel cells …”

Section: Interfacing Organic Bioelectronics With Cellsmentioning

confidence: 99%

Organic Bioelectronics for In Vitro Systems

et al. 2021

View full text Add to dashboard Cite

Bioelectronics have made strides in improving clinical diagnostics and precision medicine. The potential of bioelectronics for bidirectional interfacing with biology through continuous, label-free monitoring on one side and precise control of biological activity on the other has extended their application scope to in vitro systems. The advent of microfluidics and the considerable advances in reliability and complexity of in vitro models promise to eventually significantly reduce or replace animal studies, currently the gold standard in drug discovery and toxicology testing. Bioelectronics are anticipated to play a major role in this transition offering a much needed technology to push forward the drug discovery paradigm. Organic electronic materials, notably conjugated polymers, having demonstrated technological maturity in fields such as solar cells and light emitting diodes given their outstanding characteristics and versatility in processing, are the obvious route forward for bioelectronics due to their biomimetic nature, among other merits. This review highlights the advances in conjugated polymers for interfacing with biological tissue in vitro, aiming ultimately to develop next generation in vitro systems. We showcase in vitro interfacing across multiple length scales, involving biological models of varying complexity, from cell components to complex 3D cell cultures. The state of the art, the possibilities, and the challenges of conjugated polymers toward clinical translation of in vitro systems are also discussed throughout.

show abstract

Nanotheranostic Interface Based on Antibiotic‐Loaded Conducting Polymer Nanoparticles for Real‐Time Monitoring of Bacterial Growth Inhibition

Cited by 16 publications

References 49 publications

Preparation and Characterization of Functionalized Surgical Meshes for Early Detection of Bacterial Infections

Preparation and Characterization of Functionalized Surgical Meshes for Early Detection of Bacterial Infections

Organic Photo-antimicrobials: Principles, Molecule Design, and Applications

Organic Bioelectronics for In Vitro Systems

Contact Info

Product

Resources

About