Nanotopographic Control of Neuronal Polarity

Ferrari, Aldo; Cecchini, Marco; Dhawan, Akshay; Micera, Silvestro; Tonazzini, Ilaria; Stabile, Ripalta; Pisignano, Dario; Beltram, Fabio

doi:10.1021/nl103349s

Cited by 127 publications

(177 citation statements)

References 30 publications

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“…Conversely, small neuronal cell models, such as PC12 or SH-SY5Y (cell body diameter approx. 10 mm), followed 500 nm ridges more efficiently than larger features [31,49]. Overall, larger neuronal cells seem to respond better to larger GR periodicity.…”

Section: Discussionmentioning

confidence: 92%

“…In particular, in the last few years nanogratings-anisotropic topographies composed of alternating lines of grooves and ridges with submicrometre lateral dimensions-have been intensively investigated and have emerged as one of the most effective systems for inducing neuronal alignment via pure cell mechanotransduction. In previous studies, we showed that the interaction of nerve growth factor (NGF)-differentiating PC12 cells with these substrates promotes bipolarity and alignment to the substrate topography by interfering with FA establishment and maturation [29][30][31]. The ability to direct neurite growth depends on both the dimensions of the underlying patterned substrate and, very importantly, on the neuronal cell type [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

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Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

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Controlling and improving neuronal cell migration and neurite outgrowth are critical elements of tissue engineering applications and development of artificial neuronal interfaces. To this end, a promising approach exploits nano/microstructured surfaces, which have been demonstrated to be capable of tuning neuronal differentiation, polarity, migration and neurite orientation. Here, we investigate the neurite contact guidance of leech neurons on plastic gratings (GRs; anisotropic topographies composed of alternating lines of grooves and ridges). By high-resolution microscopy, we quantitatively evaluate the changes in tubulin cytoskeleton organization and cell morphology and in the neurite and growth cone development. The topography-reading process of leech neurons on GRs is mediated by filopodia and is more responsive to 4-µm-period GRs than to smaller period GRs. Leech neuron behaviour on GRs is finally compared and validated with several other neuronal cells, from murine differentiated embryonic stem cells and primary hippocampal neurons to differentiated human neuroblastoma cells.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

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“…[2][3][4] In fact, recent in vivo evidence [5][6][7] led to the hypothesis that axially aligned micron-scale topography which incorporates submicronscale features is required for the effective recovery of long (>20 mm) peripheral nerve defects. 8 Topography with micron-scale feature sizes is typically created via standard photolithography on suitable masking layers, whose pattern is subsequently transferred to the substrate typically through an etching process.…”

mentioning

confidence: 99%

“…These observations are consistent with the literature, where the tendency of neurites to elongate along edges 9,11 and channels 1 has been repeatedly demonstrated. Groove or ridge width appears to be the main factor determining the alignment of neurites to topographical features, where 200 nm and 500-1000 nm widths have been shown to be ideal for dorsal root ganglia neurons 4 and differentiating PC12 cells, 3 respectively. Finally, to test microfluidic integration with microtopography, we bonded a bicompartmental neuron culture device described.…”

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confidence: 99%

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Kilinc

Blasiak

Baghban

et al. 2017

Applied Physics Letters

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In vitro devices that combine chemotactic and physical cues are needed for understanding how cells integrate different stimuli. We explored the suitability of lithium niobate (LiNbO3), a transparent ferroelectric material that can be patterned with electrical charge domains and micro/nanotopography, as a neural substrate. On flat LiNbO3 z-surfaces with periodically alternating charge domains, cortical axons are partially aligned with domain boundaries. On submicron-deep etched trenches, neurites are aligned with the edges of the topographical features. Finally, we bonded a bicompartmental microfluidic chip to LiNbO3 surfaces patterned by etching, to create isolated axon microenvironments with predefined topographical cues. LiNbO3 is shown to be an emerging neuron culture substrate with tunable electrical and topographical properties that can be integrated with microfluidic devices, suitable for studying axon growth and guidance mechanisms under combined topographical/chemical stimuli.

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“…However, developmental processes such as axon pathfinding, synapse formation, nervous system patterning, neuronal plasticity, and degeneration fail to be explained solely on the basis of soluble factors. There is increasing evidence that physical variables such as the stiffness of a cellular environment influence cell development (7)(8)(9)(10)(11)(12). However, the cells of the brain tissue reside in a soft environment that is rich in polysaccharides (13,14).…”

mentioning

confidence: 99%

Stochastic nanoroughness modulates neuron–astrocyte interactions and function via mechanosensing cation channels

Blumenthal¹,

Hermanson

Heimrich

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

139

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Extracellular soluble signals are known to play a critical role in maintaining neuronal function and homeostasis in the CNS. However, the CNS is also composed of extracellular matrix macromolecules and glia support cells, and the contribution of the physical attributes of these components in maintenance and regulation of neuronal function is not well understood. Because these components possess well-defined topography, we theorize a role for topography in neuronal development and we demonstrate that survival and function of hippocampal neurons and differentiation of telencephalic neural stem cells is modulated by nanoroughness. At roughnesses corresponding to that of healthy astrocytes, hippocampal neurons dissociated and survived independent from astrocytes and showed superior functional traits (increased polarity and calcium flux). Furthermore, telencephalic neural stem cells differentiated into neurons even under exogenous signals that favor astrocytic differentiation. The decoupling of neurons from astrocytes seemed to be triggered by changes to astrocyte apical-surface topography in response to nanoroughness. Blocking signaling through mechanosensing cation channels using GsMTx4 negated the ability of neurons to sense the nanoroughness and promoted decoupling of neurons from astrocytes, thus providing direct evidence for the role of nanotopography in neuron-astrocyte interactions. We extrapolate the role of topography to neurodegenerative conditions and show that regions of amyloid plaque buildup in brain tissue of Alzheimer's patients are accompanied by detrimental changes in tissue roughness. These findings suggest a role for astrocyte and ECM-induced topographical changes in neuronal pathologies and provide new insights for developing therapeutic targets and engineering of neural biomaterials. mechanotransduction | stretch-activated channels | FAM38A | Piezo-1 | polarization

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Nanotopographic Control of Neuronal Polarity

Cited by 127 publications

References 30 publications

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Stochastic nanoroughness modulates neuron–astrocyte interactions and function via mechanosensing cation channels

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