Nanotopography in Directing Osteogenic Differentiation of Mesenchymal Stem Cells: Potency and Future Perspective

Barlian, Anggraini; Vanya, Katherine

doi:10.2144/fsoa-2021-0097

Cited by 13 publications

(8 citation statements)

References 97 publications

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“…Multiple studies have shown that NPs and other particulate materials are able to stimulate osteogenic differentiation of MSCs, inhibit adipogenesis, and are likely not to affect chondrogenesis [4][5][6][7][8][9]. The mechanisms of these effects are modernly obscure, while their better understanding may help to improve chondrocyte and osteocyte generation for clinical applications [34][35][36].…”

Section: Discussionmentioning

confidence: 99%

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Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Svirshchevskaya

Sharonova

Полтавцева

et al. 2022

IJMS

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Multiple studies have demonstrated that various nanoparticles (NPs) stimulate osteogenic differentiation of mesenchymal stem cells (MSCs) and inhibit adipogenic ones. The mechanisms of these effects are not determined. The aim of this paper was to estimate Wharton’s Jelly MSCs phenotype and humoral factor production during tri-lineage differentiation per se and in the presence of silicon–gold NPs. Silicon (SiNPs), gold (AuNPs), and 10% Au-doped Si nanoparticles (SiAuNPs) were synthesized by laser ablation, characterized, and studied in MSC cultures before and during differentiation. Humoral factor production (n = 41) was analyzed by Luminex technology. NPs were nontoxic, did not induce ROS production, and stimulated G-CSF, GM-CSF, VEGF, CXCL1 (GRO) production in four day MSC cultures. During MSC differentiation, all NPs stimulated CD13 and CD90 expression in osteogenic cultures. MSC differentiation resulted in a decrease in multiple humoral factor production to day 14 of incubation. NPs did not significantly affect the production in chondrogenic cultures and stimulated it in both osteogenic and adipogenic ones. The major difference in the protein production between osteogenic and adipogenic MSC cultures in the presence of NPs was VEGF level, which was unaffected in osteogenic cells and 4–9 times increased in adipogenic ones. The effects of NPs decreased in a row AuNPs > SiAuNPs > SiNPs. Taken collectively, high expression of CD13 and CD90 by MSCs and critical level of VEGF production can, at least, partially explain the stimulatory effect of NPs on MSC osteogenic differentiation.

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Section: Discussionmentioning

confidence: 99%

“…To visualize NPs inside the cells, MSCs were loaded with 25 µg/mL NPs for 24, fixed with 4% PFA, and analyzed by the SPNT method as described [36] using a Reichert-Jung Ultracut E ultramicrotome (Leica Microsystems GmbH, Vienna, Austria) adapted for the use of a specially designed SPM head.…”

Section: Scanning Probe Nanotomography (Spnt)mentioning

confidence: 99%

Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Svirshchevskaya

Sharonova

Полтавцева

et al. 2022

IJMS

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“…As highlighted with these examples, micro- and nanopatterns hold tremendous potential to influence SCs cell lineage decisions through biophysical signals. Nonetheless, they also display important limitations including difficult fabrication procedures which are furthermore associated with high costs . Many aspects need also to be considered such as pattern design, available technology, the choice of material, and the production costs .…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, they also display important limitations including difficult fabrication procedures which are furthermore associated with high costs. 48 Many aspects need also to be considered such as pattern design, available technology, the choice of material, and the production costs. 48 Additionally, these structures are clearly a highly simplified representation of the topographies that exist within the different tissues.…”

Section: Discussionmentioning

confidence: 99%

Silica Replicas Derived from Mammalian Cells as an Innovative Approach to Physically Direct Cell Lineage Decisions of Mesenchymal Stem Cells

Godoy-Gallardo,

Cun,

Liu

et al. 2023

ACS Appl. Mater. Interfaces

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By means of a "live-cell" template strategy, silica replicas displaying the same morphology and topography of the mammalian cells used as templates are fabricated. The replicas are used as substrates to direct the differentiation of mesenchymal stem cells (MSCs) to predefined cell lineages. Upregulation of specific genes shows how the silica replica-based substrates have the ability to induce the molecular characteristics of the mature cell types from which they have been derived from. Thus, MSCs cultured in the presence of silica replicas of human osteoblasts (HObs) differentiate into HObs-like cells, as shown by the upregulation of specific osteogenic genes. Likewise, when MSCs are incubated with silica replicas derived from human chondrocytes, an enhanced expression of chondrogenic markers is observed. Importantly, the effects of the silica replicas are cell type-specific since the incubation of MSCs with HObs silica replicas does not result in upregulation of chondrogenic markers and vice versa. What is more, for both cases, the differentiation rate is enhanced when the silica replicas are used in combination with growth factors, suggesting a potential synergistic effect. These results demonstrate the potential of this innovative method as an efficient and cheap approach with the potential to eliminate, or at least reduce, the use of biochemically soluble compounds in stem cells research.

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“…Osteogenic rough surfaces also increase the expression of Wnt5a together with the expression of the integrins in MSC ( Olivares-Navarrete et al, 2011 ). Importantly, the exogenous addition of Wnt5a and Wnt5a knock-down experiments demonstrated that the integrin upregulation was Wnt-dependent ( Olivares-Navarrete et al, 2011 ), suggesting a mutually reinforcing crosstalk between integrin adhesions and Wnt signalling ( Barlian and Vanya, 2022 ). Integrin regulates differentiation through the Wnt pathway, and Wnt increases the expression of integrin, resulting in further regulation of osteogenic differentiation ( Sun et al, 2018 ; Rougerie et al, 2021 ).…”

Section: Signaling Pathways Of Interface Morphology On Osteogenesismentioning

confidence: 99%

Effect of microtopography on osseointegration of implantable biomaterials and its modification strategies

Zhang

Fan

Xing

et al. 2022

Front. Bioeng. Biotechnol.

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Orthopedic implants are widely used for the treatment of bone defects caused by injury, infection, tumor and congenital diseases. However, poor osseointegration and implant failures still occur frequently due to the lack of direct contact between the implant and the bone. In order to improve the biointegration of implants with the host bone, surface modification is of particular interest and requirement in the development of implant materials. Implant surfaces that mimic the inherent surface roughness and hydrophilicity of native bone have been shown to provide osteogenic cells with topographic cues to promote tissue regeneration and new bone formation. A growing number of studies have shown that cell attachment, proliferation and differentiation are sensitive to these implant surface microtopography. This review is to provide a summary of the latest science of surface modified bone implants, focusing on how surface microtopography modulates osteoblast differentiation in vitro and osseointegration in vivo, signaling pathways in the process and types of surface modifications. The aim is to systematically provide comprehensive reference information for better fabrication of orthopedic implants.

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Nanotopography in Directing Osteogenic Differentiation of Mesenchymal Stem Cells: Potency and Future Perspective

Cited by 13 publications

References 97 publications

Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Silica Replicas Derived from Mammalian Cells as an Innovative Approach to Physically Direct Cell Lineage Decisions of Mesenchymal Stem Cells

Effect of microtopography on osseointegration of implantable biomaterials and its modification strategies

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