Nanotrap and Mass Analysis of Aromatic Molecules by Phenyl Group-Modified Nanoparticle

Abstract

To functionalize the surface of nanoparticles with phenyl groups for subsequent cross-linking with aromatic molecules by mutual interactions, we prepared functional nanoparticles (d = 3 nm) by silanization with phenyl-triethoxysilane. The nanoparticles had Fe(2)O(3) cores conjugated to phenyl groups; this was confirmed by Fourier transform infrared (FT-IR) spectroscopy and absorption spectrophotometry. The typical C-H and C-C peaks and the absorption at 240 nm, which corresponds to aromatic rings, were detected in the spectroscopic results for the phenyl group-modified nanoparticles. The nanoparticles could ionize aromatic (colchicine, reserpine, and bradykinin peptide) and nonaromatic (L-α-phosphatidylethanolamine,dioleoyl, and polyethylene glycol) molecules by nanoparticle-assisted laser desorption/ionization mass spectrometry. The nanoparticles worked as a selective trap and an ionization-assisting reagent in mass spectrometry for the aromatic molecular targets.

Synthesis and Characterization of Functionalized Magnetic Nanoparticles for the Detection of Pesticide

“…In the previous report, we have successfully achieved selective trapping and detection of aromatic molecules by π-π interaction [26]. In this time, we investigated whether the pyriminobac methyl as ( Figure 5(a)) pesticide is captured and detected by Ph-MNP.…”

“…Modification and coating on MNP surface does not only perform to protect MNP morphology but also serve to use functionalization with objective components, such as drug [22], enzyme [23], and amino acid [24]. It is possible that various functional groups are modified (i.e., phenyl [25], thiol [24], fluorine, chlorine, etc.) on the MNP by the silanization method.…”

“…In this research, we functionalized MNPs by the (3-aminopropyl)triethoxysilane (γ-APTES), phenyltriethoxysilane (PTES) or triethoxysilylpropyl succinic anhydride (TESPSA) to modify with amino- [2], phenyl- [26], and carboxy- [27] groups on the MNP's surface. Moreover, MNPs can be applied to analytical field.…”

“…Therefore, Nano-PALDI MS can be used in analysis of various compounds such as metabolite, drug, and oligonucleotide [29][30][31]. Especially, phenyl groupmodified (Ph-) MNPs have demonstrated the capture and ionization of target analyte by chemointeraction [26]. In this context, we investigated Ph-MNPs for the ability of recovery and detection of pesticide as a novel application for agricultural science.…”

Silver Oxide Based Nanoparticle Assisted Laser Desorption/Ionization Mass Spectrometry for the Detection of Low Molecular Weight Compounds

“…erefore, the nano-PALDI MS method can be a useful technique for the analysis of low molecular weight compounds such as metabolites, drugs and pesticides. Recent research indicates that iron-oxide based nanoparticles contribute to the speci c ionization of oligonucleotide by nano-PALDI MS. [4][5][6] e m/z of iron ion (Fe 2+ )-adduct signals increased by the mass of a transition metal ion and the number of iron ion-adducted signals depends on the oligonucleotide length in the case of iron-oxide based nano-PALDI MS. e Fe 2+ ion forms a divalent ion, but the fragment appears as a neutral charged particle due to the e ect of the phosphate group in the oligonucleotide. e Fe 2+ that is produced by the desorption process from the nanoparticles replaces the acidic protons on two negatively charged phosphate groups.…”