Nanotubular Highways for Intercellular Organelle Transport

Rustom, Amin; Saffrich, Rainer; Marković, Ivanka; Walther, Paul; Gerdes, Hans‐Hermann

doi:10.1126/science.1093133

Cited by 1,676 publications

(2,053 citation statements)

References 16 publications

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“…Some apical protrusions had local bulges or widenings, which may have been due to vesicles inside the protrusion (Fig. 1I,IЈ), as previously reported for other types of cell protrusions (Ramirez-Weber and Kornberg, 1999;Rustom et al, 2004;Hsiung et al, 2005). We conclude that wing imaginal disc columnar cells display apical protrusions that contact the squamous epithelium and that have specific morphological features.…”

Section: Apical Protrusions Of the Columnar Epithelium Contact The Apsupporting

confidence: 84%

“…Intercellular communication is important for the growth and patterning of tissues and organs and cell protrusions may mediate this communication by one of several mechanisms. Signaling mediated by cell protrusions can occur by means of transport and local release of signalling molecules, shedding of vesicles (Marzesco et al, 2005), display of receptors (Tomlinson et al, 1987;Hsiung et al, 2005) and membrane-tethered ligands on the protrusion (De Joussineau et al, 2003), or by establishing direct organelle exchange between connected cells (Rustom et al, 2004;reviewed in Demontis, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Apical and lateral cell protrusions interconnect epithelial cells in live Drosophila wing imaginal discs

Demontis

Dahmann

2007

Developmental Dynamics

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Section: Apical Protrusions Of the Columnar Epithelium Contact The Apsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Apical and lateral cell protrusions interconnect epithelial cells in live Drosophila wing imaginal discs

Demontis

Dahmann

2007

Developmental Dynamics

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“…However, several previous findings suggest cells have the capacity to explore the extracellular environment and establish direct contact with each other by migrating over long distances. Mammalian cells reportedly can build nanotubular highways for organelle transport between cells [25,29]. The telomerase activity ratios of cocultured cells to MSCs and NPCs were approximately 35.8% and 191.7%.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cell and Nucleus Pulposus Cell Coculture Modulates Cell Profile

Niu

Yuan

Lin

et al. 2008

Clin Orthop Relat Res

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Spontaneous cell fusion can occur in cocultured stem cells. We examined whether telomerase activity change and cell fusion occurred in mesenchymal stem cell (MSC) and nucleus pulposus cell (NPC) coculture. MSCs and NPCs were labeled with PKH26 and PKH67 dyes and cocultured at a 50:50 ratio. An equal number of MSCs or NPCs were used as the control. After 14 days, cells were evaluated by cell growth, telomerase activity, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and histologic observation. Cell fusion was confirmed by microscopic observation and fluorescence-activated cell sorter (FACS) analysis. The results suggested cell growth rate and telomerase activity were higher in cocultured cells than in NPCs cultured alone. The mRNA expression levels of the Type II collagen and aggrecan were elevated in cocultured cells. Immunohistochemical analysis revealed positive staining for Type II collagen and keratan sulfate in NPCs cultured alone and in a proportion of cocultured cells. Histologic observation revealed binucleated cocultured cells expressed both PKH dyes in the same location and slide focus. The FACS analysis revealed 42% of cocultured cells were doublestained. Cocultured cells partially maintained the NPC phenotype. The partially maintained phenotype of the NPCs may be attributable to spontaneous cell fusion in association with increased telomerase activity.

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“…In addition to exosomes, tunnelling nanotubes -long, intercellular connections of 50-700 nm in diameter prov iding membrane continuity between connected cells -have emerged as an alternative means of transfer of microRNAs and even organelles 146,147 . Collectively, these means of intercellular communication influence cardiac remodelling and modulate loss of cardiac function.…”

Section: Nature Reviews | Cardiologymentioning

confidence: 99%

Proteostasis in cardiac health and disease

Henning

Brundel²

2017

Nat Rev Cardiol

144

126

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The worldwide increase in life expectancy gives rise to an increasing prevalence of cardiac diseases, which remain the leading cause of disability and death in the developed world [1][2][3] . Currently, the mechanisms under lying how ageing contributes to the initiation or acceler ation of cardiac diseases are essentially unresolved. Prevailing theories of ageing centre on gradual derail ment of cellular protein homeostasis -proteostasis -either by design (genetically) or by 'wear and tear' (environmentally) 3 . Central to the role of proteostasis derailment as a major factor in ageing is the observation that protein function declines over time.Proteins are the most versatile and complex macro molecules and are involved in the proper functioning of every biological process 4 . After synthesis as a poly peptide chain from the genetic code, proper function of a protein relies heavily on its correct folding into a 3D native state and on various post translational modifi cations, mostly involving the addition of chem ical groups (including phosphate, acetate, and carbo hydrate). Protein maturation, transport, and ultimately breakdown is monitored and supported by various classes of proteins, collectively called 'protein quality control' (PQC) [3][4][5] . Balanced proteostasis, therefore, depends on proper PQC and is crucial for cellular and organismal health 6 . The intricate network making up the PQC involves numerous proteins, of which the main players are the chaperones and their regula tors 4,7-9 (assisting in folding and refolding of proteins) and the pathways that clear irreversibly misfolded and aggregation prone proteins (the ubiquitin-proteasome system [UPS] and autophagy systems) 9-11 . With ageing, the gradual accumulation of misfolded or damaged pro teins, together with concomitant failure of PQC, results in proteotoxic stress and compromised defence against reactive oxygen species (ROS) 10 , a process that is likely to be accelerated in various age related diseases includ ing neurodegenerative diseases 12,13 , type 2 diabetes mel litus 14 , cancer 15 , and cardiac diseases [16][17][18][19][20] . In addition to the accumulation of misfolded proteins, ageing is accompanied by an imbalance in the proteome, as indi cated by lowered expression of chaperone, proteaso mal, ribosomal, and mitochondrial proteins, whereas proteins related to oxidative stress are upregulated 21,22 . Although mild impairment of proteostasis extends lifespan in Caenorhabditis elegans, referred to as hormesis, sustained impairment is accompanied by loss of PQC to neutralize this effect 3,5 . Importantly, evidence indicates that the amount of soluble, aggregate prone protein oligomers, rather than the abundance of pro tein aggregates, correlates with disease severity 23,24 . Therefore, protein aggregates, which contain both misfolded proteins and elevated levels of chaper ones, constitute an adequate PQC response, aimed at sequestering potentially harmful protein oligomers, rather than embodying the ultimate cellular threat 25 . This ...

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Nanotubular Highways for Intercellular Organelle Transport

Cited by 1,676 publications

References 16 publications

Apical and lateral cell protrusions interconnect epithelial cells in live Drosophila wing imaginal discs

Apical and lateral cell protrusions interconnect epithelial cells in live Drosophila wing imaginal discs

Mesenchymal Stem Cell and Nucleus Pulposus Cell Coculture Modulates Cell Profile

Proteostasis in cardiac health and disease

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