NanoUPLC/MSE proteomic analysis reveals modulation on left ventricle proteome from hypertensive rats after exercise training

Petriz, Bernardo A.; Almeida, Jeeser Alves de; Gomes, Clarissa P.C.; Pereira, Rinaldo Wellerson; Murad, André M.; Franco, Octávio L.

doi:10.1016/j.jprot.2014.10.010

Cited by 16 publications

(10 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The inclusion criteria for identified proteins were the presence in at least four of five animals from each group. The proteins were grouped in biological process class according to Petriz et al [17].…”

Section: Offspring Blood Sample Collection and Biochemicalmentioning

confidence: 99%

“…Although multiple studies determined that chronic exercise can be a crucial factor in transgenerational inheritance, the molecular mechanisms underlying cardiovascular benefits generated from exercise training remain to be investigated. In this sense, proteomics is an efficient technique with satisfactory precision that estimates a large number of proteins at the same time and represents an import method to clarify more profoundly the molecular networks behind physiological adaptations promoted by exercise training [17]. In a recent study, UPLC-MSE proteomics revealed modulation on the LV proteome from rats submitted to resistance training (RT) [18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Paternal Resistance Training Induced Modifications in the Left Ventricle Proteome Independent of Offspring Diet

Neto

Tibana

Prestes

et al. 2020

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Ancestral obesogenic exposure is able to trigger harmful effects in the offspring left ventricle (LV) which could lead to cardiovascular diseases. However, the impact of the father’s lifestyle on the offspring LV is largely unexplored. The aim of this study was to investigate the effects of 8 weeks of paternal resistance training (RT) on the offspring left ventricle (LV) proteome exposed to control or high-fat (HF) diet. Wistar rats were randomly divided into two groups: sedentary fathers and trained fathers (8 weeks, 3 times per week with weights secured to the animals’ tails). The offspring were obtained by mating with sedentary females. Upon weaning, male offspring were divided into 4 groups (5 animals per group): offspring from sedentary fathers, exposed to control diet (SFO-C); offspring from trained fathers, exposed to control diet (TFO-C); offspring from sedentary fathers, exposed to high-fat diet (SFO-HF); and offspring from trained fathers, exposed to high-fat diet (TFO-HF). The LC-MS/MS analysis revealed 537 regulated proteins among groups. Offspring exposure to HF diet caused reduction in the abundance levels of proteins related to cell component organization, metabolic processes, and transport. Proteins related to antioxidant activity, transport, and transcription regulation were increased in TFO-C and TFO-HF as compared with the SFO-C and SFO-HF groups. Paternal RT demonstrated to be an important intervention capable of inducing significant effects on the LV proteome regardless of offspring diet due to the increase of proteins involved into LV homeostasis maintenance. This study contributes to a better understanding of the molecular aspects involved in transgenerational inheritance.

show abstract

Section: Offspring Blood Sample Collection and Biochemicalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Paternal Resistance Training Induced Modifications in the Left Ventricle Proteome Independent of Offspring Diet

Neto

Tibana

Prestes

et al. 2020

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…It has long been described as a biomarker for ageing in the skeletal muscle of female humans [ 45 ]. More recently, this protein has also been noted to play a significant role in ovine Corpus Luteum regression [ 46 ] and has been shown to increase expression levels in the ventricle proteome of hypertensive rats in response to exercise [ 47 ]. It also increases expression in glycolytic muscle of leptin-deficient obese mice, albeit while maintaining expression levels in oxidative muscle [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Weight Loss on the Muscle Proteome in the Damara, Dorper and Australian Merino Ovine Breeds

Almeida

Palhinhas

Kilminster³

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Seasonal Weight Loss (SWL) is an important constraint, limiting animal production in the Tropics and the Mediterranean. As a result, the study of physiological and biochemical mechanisms by which domestic animal breeds respond to SWL is important to those interested in animal breeding and the improvement thereof. To that end, the study of the proteome has been instrumental in gathering important information on physiological mechanisms, including those underlying SWL. In spite of that, little information is available concerning physiological mechanisms of SWL in production animals. The objective of this study was to determine differential protein expression in the muscle of three different breeds of sheep, the Australian Merino, the Dorper and the Damara, each showing different levels of tolerance to weight loss (low, medium and high, respectively). Per breed, two experimental groups were established, one labeled “Growth” and the other labeled “Restricted.” After forty-two days of dietary treatment, all animals were euthanized. Muscle samples were then taken. Total protein was extracted from the muscle, then quantified and two-dimensional gel electrophoresis were conducted using 24 cm pH 3–10 immobiline dry strips and colloidal coomassie staining. Gels were analyzed using Samespots® software and spots of interest were in-gel digested with trypsin. The isolated proteins were identified using MALDI-TOF/TOF. Results indicated relevant differences between breeds; several proteins are suggested as putative biomarkers of tolerance to weight loss: Desmin, Troponin T, Phosphoglucomutase and the Histidine Triad nucleotide-binding protein 1. This information is of relevance to and of possible use in selection programs aiming towards ruminant animal production in regions prone to droughts and weight loss.

show abstract

“…Tryptic peptides were analyzed using a nanoACQUITY™ system (Waters®) with 2D dilution technology coupled to a Synapt G2 HDMS™ mass spectrometer (Waters®) following procedures and settings described in Petriz et al. . The protein identification criteria included the detection of at least one fragment ion per peptide, three peptides per protein, the determination of at least one peptide per protein and the identification of the protein was allowed with a maximum 4% false‐positive discovery rate.…”

Section: Methodsmentioning

confidence: 99%

Differential accumulation of Xanthomonas campestris pv. campestris proteins during the interaction with the host plant: Contributions of an in vivo system

et al. 2017

Self Cite

View full text Add to dashboard Cite

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot, a highly destructive disease that affects all brassicas. This work aimed to study the interaction Xcc-Brassica oleracea using an in vivo system in an attempt to identify proteins involved in pathogenicity. We used label-free shotgun 2D-nanoUPLC/MS to analyze Xcc proteins in three conditions: in the interaction with susceptible (REK) and resistant (REU) plants and in culture medium (control condition). A model of Xcc-susceptible host interaction is proposed and shows that Xcc increases the abundance of several crucial proteins for infection and cell protection. In this study, we also confirmed the differential expression by qPCR analysis of selected genes. This is the first report showing a large-scale identification of proteins in an in vivo host plant condition. Considering that most studies involving phytopathogens are in vitro (growth in culture medium or in plant extract), this work contributes with relevant information related to the plant-pathogen interaction in planta.

show abstract

NanoUPLC/MSE proteomic analysis reveals modulation on left ventricle proteome from hypertensive rats after exercise training

Cited by 16 publications

References 111 publications

Paternal Resistance Training Induced Modifications in the Left Ventricle Proteome Independent of Offspring Diet

Paternal Resistance Training Induced Modifications in the Left Ventricle Proteome Independent of Offspring Diet

The Effect of Weight Loss on the Muscle Proteome in the Damara, Dorper and Australian Merino Ovine Breeds

Differential accumulation of Xanthomonas campestris pv. campestris proteins during the interaction with the host plant: Contributions of an in vivo system

Contact Info

Product

Resources

About