Nanowire Arrays as Force Sensors with Super‐Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces

Silva, Aldeliane M. da; Sahoo, Prativa; Cavalli, Alessandro; Souza, Alessandra Alves de; Bakkers, Erik P. A. M.; César, Carlos L.; Janissen, Richard; Cotta, M. A.

doi:10.1002/smtd.201700411

Cited by 13 publications

(16 citation statements)

References 93 publications

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“…While a number of studies have begun to explore the interaction between nanoscale geometry and bacterial cells, much remains unexplored. Similar to eukaryotic techniques, Cotta and co‐workers have used indium phosphide nanowire arrays to measure the piconewton adhesion forces exerted by bacterial cells ( Xylella fastidiosa ) …”

Section: Prokaryotic Cell Interfacingmentioning

confidence: 99%

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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Materials patterned with high‐aspect‐ratio nanostructures have features on similar length scales to cellular components. These surfaces are an extreme topography on the cellular level and have become useful tools for perturbing and sensing the cellular environment. Motivation comes from the ability of high‐aspect‐ratio nanostructures to deliver cargoes into cells and tissues, access the intracellular environment, and control cell behavior. These structures directly perturb cells' ability to sense and respond to external forces, influencing cell fate, and enabling new mechanistic studies. Through careful design of their nanoscale structure, these systems act as biological metamaterials, eliciting unusual biological responses. While predominantly used to interface eukaryotic cells, there is growing interest in nonanimal and prokaryotic cell interfacing. Both experimental and theoretical studies have attempted to develop a mechanistic understanding for the observed behaviors, predominantly focusing on the cell–nanostructure interface. This review considers how high‐aspect‐ratio nanostructured surfaces are used to both stimulate and sense biological systems.

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Section: Prokaryotic Cell Interfacingmentioning

confidence: 99%

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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“…Another strategy to improve the resolution of elastic pillar arrays is to replace the PDMS posts with a nanowire array (Figure n). Metal nanowire arrays have been successfully used to measure cell-generated forces, particularly in bacteria , and cancer cells . Because of their small diameter (<100 nm), nanowire arrays can be fabricated at a high density, increasing the resolution.…”

Section: Measuring Forces Exerted By Cellsmentioning

confidence: 99%

“…Because of their small diameter (<100 nm), nanowire arrays can be fabricated at a high density, increasing the resolution. Detection of the nanowire tips represents the main difficulty of this technique, and different methods have been explored, including field-emission scanning electron microscopy (FESEM), confocal laser scanning fluorescence microscopy (CLSM), , and photoluminiscent tips . It is currently unclear whether cells cultured on nanowire arrays can form adequate-sized FA, given the small-sized (<100 nm) and discontinuous nature of the pillars, although traction forces generated are in agreement with other micropillar arrays sytems. , Further research is required to characterize the interaction between cells and these nanowires.…”

Section: Measuring Forces Exerted By Cellsmentioning

confidence: 99%

Where No Hand Has Gone Before: Probing Mechanobiology at the Cellular Level

Matellan

Hernández

2018

ACS Biomater. Sci. Eng.

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Physical forces and other mechanical stimuli are fundamental regulators of cell behavior and function. Cells are also biomechanically competent: they generate forces to migrate, contract, remodel, and sense their environment. As the knowledge of the mechanisms of mechanobiology increases, the need to resolve and probe increasingly small scales calls for novel technologies to mechanically manipulate cells, examine forces exerted by cells, and characterize cellular biomechanics. Here, we review novel methods to quantify cellular force generation, measure cell mechanical properties, and exert localized piconewton and nanonewton forces on cells, receptors, and proteins. The combination of these technologies will provide further insight on the effect of mechanical stimuli on cells and the mechanisms that convert these stimuli into biochemical and biomechanical activity.

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“…To evaluate the surface properties, we chose SU-8 pristine and oxygen plasma treated (for 60 seconds) SU-8 samples, based on our optimized protocol for surface modifications that provides minimal surface roughness [5]. In addition, flat Indium Phosphide (InP) substrates are also chosen for reference control since the X. fastidiosa bacterial adhesion to InP surfaces has been well studied [49,50]. Initially, RMS surface (which was not certified by peer review) is the author/funder.…”

Section: Resultsmentioning

confidence: 99%

Physiochemically distinct SU-8 surfaces tailor Xylella fastidiosa cell-surface holdfast and colonization

Anbumani

Silva

Alaferdov

et al. 2021

Preprint

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SU-8 polymer is an excellent platform for diverse applications due to its high aspect ratio of micro/nanostructures fabrication and exceptional optical, chemical, and biocompatible properties. Although SU-8 has been often investigated for a variety of biological applications, how its surface properties influence both the interaction of bacterial cells with the substrate and its colonization is poorly understood. In this work, we tailor SU-8 nanoscale surface properties to investigate single cell motility, adhesion and successive colonization of a phytopathogenic bacteria, Xylella fastidiosa. Different surface properties of SU-8 thin films have been prepared using photolithography processing and oxygen plasma treatment. We found a significant difference in bacterial cell behavior and subsequent colonization on SU-8 as surface property changes. A larger density of carboxyl groups in hydrophilic plasma-treated SU-8 surfaces promotes faster cell motility in the earlier stage of the growth. The hydrophobic nature of pristine SU-8 surfaces has no trackable bacterial motility with 5 to 10 times more single cells adhered to surface than its plasma-treated counterpart. In fact, plasma-treated SU-8 samples suppressed bacterial adhesion, with surfaces showing less than 5% coverage. These results not only showcase that SU-8 surface properties can impact the bacterial behavior in a spatiotemporal manner, but also provide insights on the prominent ability of pathogens to evolve and adapt to different surface properties.

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Nanowire Arrays as Force Sensors with Super‐Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces

Cited by 13 publications

References 93 publications

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

Where No Hand Has Gone Before: Probing Mechanobiology at the Cellular Level

Physiochemically distinct SU-8 surfaces tailor Xylella fastidiosa cell-surface holdfast and colonization

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