Nanozyme-Based Sensors for Pesticide Detection

Single-step colorimetric detection of acid phosphatase in human urine using an oxidase-mimic platinum nanozyme

“…17 Recent research has seen the use of nanomaterials that mimic the catalytic activity of natural enzymes, commonly called nanozymes, to generate a colorimetric output in sensing applications. [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] The use of nanomaterials instead of a natural enzyme such as HRP to catalyse enzymatic reactions have advantages, such as the ability to operate in a wider range of conditions, ease of modifying the surface chemistry of nanoparticles to control its catalytic properties, and facile conjugation of recognition moieties to improve sensor selectivity. 19,[35][36][37][38] So far, nanozymes with the ability to mimic the catalytic activity of oxidoreductases, hydrolases, lyases, and isomerases have been reported.…”

“…32 Among different enzyme mimics, much of the work has focused on oxidoreductases, primarily on peroxidases and oxidases. 19,32,33 A key difference between peroxidase and oxidase nanozymes is that the former requires hydrogen peroxide (H 2 O 2 ) as a co-substrate to catalyse the oxidation of organic substrates. 41 In contrast, oxidase-mimic nanozymes can use ambient O 2 as an electron acceptor.…”